Inhibition of HIV Type 1 Replication by Simultaneous Infection of Peripheral Blood Lymphocytes with Human Immunodeficiency Virus Types 1 and 2

2001 ◽  
Vol 17 (4) ◽  
pp. 295-309 ◽  
Author(s):  
Kirsten Dern ◽  
Helga Rübsamen-Waigmann ◽  
Ronald E. Unger
Keyword(s):  
Human Immunodeficiency Virus ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Immunodeficiency Virus ◽  
Simultaneous Infection ◽  
Hiv Type 1

scholarly journals Human Immunodeficiency Virus Type 1 Modulates Telomerase Activity in Peripheral Blood Lymphocytes

2001 ◽  
Vol 183 (3) ◽  
pp. 417-424 ◽  
Author(s):  
Gianna Ballon ◽  
Lucia Ometto ◽  
Elisabetta Righetti ◽  
Anna Maria Cattelan ◽  
Sara Masiero ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Peripheral Blood ◽  
Telomerase Activity ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Immunodeficiency Virus

scholarly journals Anti-Human Immunodeficiency Virus Activity and Cellular Metabolism of a Potential Prodrug of the Acyclic Nucleoside Phosphonate 9-R-(2-Phosphonomethoxypropyl)adenine (PMPA), Bis(isopropyloxymethylcarbonyl)PMPA

1998 ◽  
Vol 42 (3) ◽  
pp. 612-617 ◽  
Author(s):  
Brian L. Robbins ◽  
Ranga V. Srinivas ◽  
Choung Kim ◽  
Norbert Bischofberger ◽  
Arnold Fridland
Keyword(s):  
Human Immunodeficiency Virus ◽  
Peripheral Blood ◽  
Active Metabolite ◽  
Peripheral Blood Lymphocytes ◽  
Activated Lymphocytes ◽  
Blood Lymphocytes ◽  
Virus Activity ◽  
Immunodeficiency Virus ◽  
Resting Lymphocytes ◽  
Pharmacologically Active

ABSTRACT Bis(isopropyloxymethylcarbonyl) 9-R-(2-phosphonomethoxypropyl)adenine [bis(POC)PMPA] has been identified as a novel prodrug of PMPA. The anti-human immunodeficiency virus activity of bis(POC)PMPA was >100-fold greater than that of PMPA in both an established T-cell line and primary peripheral blood lymphocytes. This improved efficacy was shown to be due to a rapid intracellular uptake of the prodrug resulting in an increased intracellular accumulation of PMPA diphosphate (PMPApp), the pharmacologically active metabolite. PMPApp levels in bis(POC)PMPA-treated cells exceeded by >1,000-fold the levels seen in cells treated with unmodified PMPA in both resting and activated peripheral blood lymphocytes. Significant differences in the intracellular catabolism of PMPA metabolites were noted between the resting and activated lymphocytes. The half-life for the disappearance of PMPApp, derived from either bis(POC)PMPA or PMPA, was 12 to 15 h in the activated lymphocytes and 33 to 50 h in the resting lymphocytes. This long persistence of PMPApp, particularly in resting lymphocytes, may be unique to the nucleoside phosphonate analogs and indicates that effective levels of the active metabolite can be achieved and maintained with relatively infrequent administration of the parent drug.

scholarly journals Persistence of Human Immunodeficiency Virus (HIV) Type 1 DNA in Peripheral Blood Despite Prolonged Suppression of Plasma HIV‐1 RNA in Children

2002 ◽  
Vol 185 (10) ◽  
pp. 1409-1416 ◽  
Author(s):  
Akihiko Saitoh ◽  
Karen Hsia ◽  
Terence Fenton ◽  
Christine A. Powell ◽  
Cindy Christopherson ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Peripheral Blood ◽  
Immunodeficiency Virus ◽  
Hiv Type 1 ◽  
Hiv 1
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