Filter-Paper Electrophoresis of Serum Proteins from Small Animals

1953 ◽  
Vol 26 (2) ◽  
pp. 95-100 ◽  
Author(s):  
Thomas L. Gleason ◽  
Felix Friedberg
Keyword(s):  
Filter Paper ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Small Animals

Filter Paper Electrophoresis of Serum Proteins from Sharks

Copeia ◽  
1957 ◽  
Vol 1957 (4) ◽  
pp. 292 ◽  
Author(s):  
Theodore J. Starr ◽  
Walter Fosberg
Keyword(s):  
Filter Paper ◽  
Serum Proteins ◽  
Paper Electrophoresis

FILTER PAPER ELECTROPHORESIS OF SERUM PROTEINS IN CLINICAL PRACTICE

1954 ◽  
Vol 3 (4) ◽  
pp. 270-278 ◽  
Author(s):  
E. G. MCQUEEN ◽  
R. F. O'SHEA ◽  
M. P. SUMMERFIELD
Keyword(s):  
Clinical Practice ◽  
Filter Paper ◽  
Serum Proteins ◽  
Paper Electrophoresis

scholarly journals ELECTROPHORESIS OF PROTEINS ON FILTER PAPER

1951 ◽  
Vol 35 (1) ◽  
pp. 89-118 ◽  
Author(s):  
Henry G. Kunkel ◽  
Arne Tiselius
Keyword(s):  
Serum Albumin ◽  
Filter Paper ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Normal Serum ◽  
Concentration Gradients ◽  
Simplified Procedure ◽  
Highly Porous ◽  
Electrophoresis Of Proteins ◽  
Serum Components

A simplified procedure for filter paper electrophoresis is described in which disturbing factors such as evaporation, heating, buffer concentration gradients, and pH changes in the electrode vessels were reduced to a minimum. Artificial mixtures of highly purified proteins could be separated and the components isolated. The application of the method to a variety of studies on serum proteins is demonstrated. Protein concentration in paper segments was determined by two different methods of protein estimation. Curves were obtained showing the same five major peaks for normal serum as found by the classical methods of free electrophoresis. Comparisons were made of the areas of the various components under the curves obtained with the different methods. Two dimensional electrophoresis was applied to serum and serum components. It proved of value in demonstrating the heterogeneity of fractions such as the γ-globulin of serum. The polysaccharide dextran was used as an index of the extent of electro-osmotic flow during the course of the various experiments. The ratio of the distance of electroosmotic flow and the distance of protein migration was shown to be constant for a given type of paper. For serum albumin on Munktell 20 paper this ratio was 0.35. A formula for mobilities applicable to liquid in a highly porous supporting medium is presented. Mobility values for human serum albumin at various pH levels on paper showed approximate agreement with those obtained in free solution giving a similar isoelectric point.

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

1954 ◽  
Vol 32 (1) ◽  
pp. 189-199 ◽  
Author(s):  
W. P. McKinley ◽  
W. A. Maw ◽  
W. F. Oliver ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Filter Paper ◽  
Domestic Fowl ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

Filter Paper Electrophoresis of Avian Serum Proteins

Science ◽  
1953 ◽  
Vol 118 (3055) ◽  
pp. 86-89 ◽  
Author(s):  
R. H. Common ◽  
W. P. McKinley ◽  
W. A. Maw
Keyword(s):  
Filter Paper ◽  
Serum Proteins ◽  
Paper Electrophoresis

Filter Paper Electrophoresis of Serum Proteins of the Domestic Fowl.

1953 ◽  
Vol 84 (2) ◽  
pp. 346-351 ◽  
Author(s):  
W. P. McKinley ◽  
W. F. Oliver ◽  
W. A. Maw ◽  
R. H. Common
Keyword(s):  
Filter Paper ◽  
Domestic Fowl ◽  
Serum Proteins ◽  
Paper Electrophoresis

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

1954 ◽  
Vol 32 (3) ◽  
pp. 189-199 ◽  
Author(s):  
W. P. McKinley ◽  
W. A. Maw ◽  
W. F. Oliver ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Filter Paper ◽  
Domestic Fowl ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

Cholesterol in Serum and Lipoprotein Fractions

1956 ◽  
Vol 2 (3) ◽  
pp. 145-159 ◽  
Author(s):  
Joseph T Anderson ◽  
Ancel Keys
Keyword(s):  
Human Serum ◽  
Total Cholesterol ◽  
Filter Paper ◽  
Room Temperature ◽  
Paper Electrophoresis ◽  
Cholesterol Content ◽  
Intraindividual Variability ◽  
Detailed Data ◽  
The Stability ◽  
Source Of Error

Abstract 1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.

Localization of Protein-bound Radioactive Iodine by Filter Paper Electrophoresis

Science ◽  
1952 ◽  
Vol 115 (2997) ◽  
pp. 626-627 ◽  
Author(s):  
F. Larson ◽  
W. P. Deiss ◽  
E. C. Albright
Keyword(s):  
Filter Paper ◽  
Radioactive Iodine ◽  
Paper Electrophoresis
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