The Fifth Consensus Conference on Testing for Human Retroviruses

1990 ◽  
Vol 11 (8) ◽  
pp. 417-418
Author(s):  
William J. Hausler ◽  
Jane P. Getchell
Keyword(s):  
Public Health ◽  
Human Immunodeficiency Virus ◽  
Western Blot ◽  
Data Sharing ◽  
Immunosorbent Assay ◽  
Consensus Conference ◽  
Enzyme Linked Immunosorbent Assay ◽  
Test Procedures ◽  
Hiv Test ◽  
Immunodeficiency Virus

In late 1985, the Association of State and Territorial Public Health Laboratory Directors (ASTPHLD) first recognized the need for standardization and uniformity of human immunodeficiency virus (HIV) test procedures, interpretation and reporting among public health laboratories. In 1986, the HIV Committee of ASTPHLD conducted its first Consensus Conference, with the objective of promoting quality and uniformity among laboratory programs through the exchange of data, sharing of expertise and formulation of consensus recommendations. There were 41 participants at this conference that addressed four topics: enzyme-linked immunosorbent assay (ELISA), Western blot, immunofluorescence and methods standardization.

scholarly journals Comparison of Indirect Immunofluorescence Assay as a Confirmatory Test to Detect Antibody to Human Immunodeficiency Virus with Line Imuune Assay and Western Blot Methods

2016 ◽  
Vol 2 (2) ◽  
pp. 12-16
Author(s):  
Ahmed Nawsher Alam ◽  
Mahmuda Siddiqua ◽  
Shahina Tabassum ◽  
Md Nazrul Islam
Keyword(s):  
Human Immunodeficiency Virus ◽  
Western Blot ◽  
Indirect Immunofluorescence ◽  
Immunofluorescence Assay ◽  
Indirect Immunofluorescence Assay ◽  
Confirmatory Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunodeficiency Virus ◽  
Positive Serum ◽  
Rapid Tests

In low prevalent countries of Human Immunodeficiency Virus (HIV) like Bangladesh, it is recommended that all HIVpositive sera tested by Enzyme Linked Immunosorbent Assay (ELISA) and/or rapid tests should be confirmed by Line Immune Assay (LIA) or Western Blot (WB) method. As these two tests are quite expensive, Indirect Immunofluorescence Assay (IFA) was evaluated as a confirmatory test and as an alternative to these two methods in the present study. A total of 92 subjects consisting of 46 HIV-antibody-positive patients and 46 controls were included in the study. All samples of sera were tested by ELISA and IFA methods, and some 34 of 46 HIV-antibody-positive samples were tested by LIA. One ELISA positive serum was found negative by both IFA and LIA. This result indicates that ELISA was 100% sensitive and 98.7% specific for detection of HIV antibody. Comparison between LIA with IFA on 34 samples, and WB with IFA on 26 samples showed 100% correlation between these methods. The study concluded that the IFA method is equal in performance as LIA and WB methods for the detection of antibody to HIV and can be used as a confirmatory test.

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