Molecular Typing ofMycobacterium chelonaeIsolates From a Pseudo-outbreak Involving an Automated Bronchoscope Washer

2008 ◽  
Vol 29 (11) ◽  
pp. 1088-1090 ◽  
Author(s):  
Alexandra Chroneou ◽  
Sarah K. Zimmerman ◽  
Steven Cook ◽  
Sandra Willey ◽  
Jane Eyre-Kelly ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Molecular Typing ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Clinical Findings ◽  
Chain Reaction ◽  
Mycobacterium Chelonae ◽  
Polymerase Chain ◽  
Repetitive Extragenic Palindromic

We describe a pseudo-outbreak ofMycobacterium chelonaeinfection in bronchoalveolar lavage fluid from 9 patients that was traced to contamination of an automated bronchoscope washer. Molecular typing using repetitive extragenic palindromic polymerase chain reaction was helpful in confirming epidemiologic and clinical findings.

Role of Cytology and Polymerase Chain Reaction Based Detection of Pneumocystis jirovecii Infection in Bronchoalveolar Lavage Fluid

2010 ◽  
Vol 54 (3) ◽  
pp. 296-302 ◽  
Author(s):  
Rashmi Gupta ◽  
Venkateswaran K. Iyer ◽  
Bijay Ranjan Mirdha ◽  
Randeep Guleria ◽  
Lalit Kumar ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Pneumocystis Jirovecii ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Nested–polymerase chain reaction detection of Pneumocystis carinii f. sp. canis in a suspected immunocompromised Cavalier King Charles spaniel with multiple infections

2019 ◽  
Vol 7 ◽  
pp. 2050313X1984116
Author(s):  
Matteo Petini ◽  
Tommaso Furlanello ◽  
Patrizia Danesi ◽  
Andrea Zoia
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Pneumocystis Carinii ◽  
Lavage Fluid ◽  
Chain Reaction ◽  
Cavalier King Charles Spaniel ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain ◽  
Tract Infections

A 7-month-old Cavalier King Charles Spaniel female was referred due to a chronic cough refractory to antibiotic treatments. Laboratory findings showed leukocytosis, increased serum C-reactive protein, hypogammaglobulinemia, and decreased total serum immunoglobulin G concentration. Thoracic radiographs showed a mild bronchial pattern. Cytology of the bronchoalveolar lavage fluid revealed a septic inflammation. Bordetella bronchiseptica, Mycoplasma spp., and Pneumocystis carinii were identified by polymerase chain reaction testing, and Klebsiella pneumonia was cultured from the bronchoalveolar lavage fluid. Moreover, Escherichia coli was also cultured from urine. Pneumocystis spp. identification was done by sequencing of genetic amplicons. The dog died due to cardiopulmonary arrest secondary to a spontaneous pneumothorax on the day following the procedure. This report documents the detection of Pneumocystis carinii f. sp. canis in a suspected immunocompromised Cavalier King Charles Spaniel with concurrent pulmonary and urinary tract infections involving four different pathogens, and highlights the importance of the use of polymerase chain reaction testing to detect canine Pneumocystis spp. in cases with negative bronchoalveolar lavage cytology.

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