scholarly journals Frequent Detection of Respiratory Viruses in Adult Recipients of Stem Cell Transplants with the Use of Real-Time Polymerase Chain Reaction, Compared with Viral Culture

2005 ◽  
Vol 40 (5) ◽  
pp. 662-669 ◽  
Author(s):  
M. G. J. van Kraaij ◽  
L. J. R. van Elden ◽  
A. M. van Loon ◽  
K. A. W. Hendriksen ◽  
L. Laterveer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Stem Cell ◽  
Real Time ◽  
Respiratory Viruses ◽  
Chain Reaction ◽  
Viral Culture ◽  
Cell Transplants ◽  
Polymerase Chain ◽  
Stem Cell Transplants

Quantitative assessment of hematopoietic chimerism after bone marrow transplantation by real-time quantitative polymerase chain reaction

Blood ◽  
2002 ◽  
Vol 99 (12) ◽  
pp. 4618-4625 ◽  
Author(s):  
Mehdi Alizadeh ◽  
Marc Bernard ◽  
Bruno Danic ◽  
Charly Dauriac ◽  
Brigitte Birebent ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Stem Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Assessment ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Hematopoietic Stem ◽  
Pcr Method ◽  
Polymerase Chain

We have developed a real-time quantitative polymerase chain reaction (PCR) assay using TaqMan technology (Applied Biosystems, Foster City, CA) for monitoring donor cell engraftment in allogenic hematopoietic stem cell transplant recipients. For this purpose, we selected 19 specific sequence polymorphisms belonging to 11 human biallelic loci located on 9 different chromosomes. Using a set of specially designed primers and fluorogenic probes, we evaluated the 19 markers' informativity on a panel of 126 DNA samples from 63 recipient/donor pairs. In more than 90% of these pairs, discrimination between recipient and donor genetic profile was possible. By using serial dilutions of mixed DNAs, we evaluated the linearity and sensitivity of the method. A linear correlation with rhigher than 0.98 and a sensitivity of 0.1% proved reproducible. Fluorescent-based PCR of short tandem repeats (STR-PCR) and real-time PCR chimerism assay were compared with a panel of artificial cell mixtures. The main advantage of the real-time PCR method over STR-PCR chimerism assays is the absence of PCR competition and plateau biases, and results evidenced greater sensitivity and linearity with the real-time PCR method. Furthermore, different samples can be tested in the same PCR run with a final result in fewer than 48 hours. Finally, we prospectively analyzed patients who received allografts and present 4 different clinical situations that illustrate the informativity level of our method. In conclusion, this new assay provides an accurate quantitative assessment of mixed chimerism that can be useful in guiding early implementation of additional treatments in hematopoietic stem cell transplantation.

scholarly journals Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens

2019 ◽  
Vol 22 (2) ◽  
pp. 35
Author(s):  
Jean Damascene Uwizeyimana ◽  
Min Kyung Kim ◽  
Daewon Kim ◽  
Jung-Hyun Byun ◽  
Dongeun Yong
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Respiratory Viruses ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of respiratory viruses and Legionella spp. by real-time polymerase chain reaction in patients with community acquired pneumonia

2009 ◽  
Vol 41 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Bram M.W. Diederen ◽  
Menno M. Van Der Eerden ◽  
Fer Vlaspolder ◽  
Wim G. Boersma ◽  
Jan A.J.W. Kluytmans ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Respiratory Viruses ◽  
Community Acquired Pneumonia ◽  
Chain Reaction ◽  
Polymerase Chain
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