GFP‐Targeted Mitochondria Show Heterogeneity of Size, Morphology, and Dynamics in Transgenic Nicotiana tabacum L. Plants In Vivo

2004 ◽  
Vol 165 (6) ◽  
pp. 949-955 ◽  
Author(s):  
Yih‐Shan Lo ◽  
Lin‐June Hsiao ◽  
Wann‐Neng Jane ◽  
Yuh‐Cheng Charng ◽  
Hwa Dai ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L ◽  
Transgenic Nicotiana Tabacum

Stability of a foreign gene in transgenic Nicotiana tabacum L. plants during a cycle of dedifferentiation/differentiation

Plant Science ◽  
1992 ◽  
Vol 82 (2) ◽  
pp. 193-200 ◽  
Author(s):  
Catherine Bellini ◽  
Chiara Giordani ◽  
Elisabetta Lupotto ◽  
Franca Locatelli ◽  
Elena Cuzzoni ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Foreign Gene ◽  
Nicotiana Tabacum L ◽  
Transgenic Nicotiana Tabacum

Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase overexpression in transgenic tobacco: physiological and biochemical analysis of source and sink tissues

2002 ◽  
Vol 80 (9) ◽  
pp. 983-992 ◽  
Author(s):  
Susan M Wood ◽  
Steven P King ◽  
Monika M Kuzma ◽  
Stephen D Blakeley ◽  
William Newcomb ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Total Biomass ◽  
Native Plant ◽  
Morphological Alterations ◽  
Young Embryo ◽  
Nicotiana Tabacum L ◽  
Transgenic Seeds ◽  
High Level ◽  
Source And Sink ◽  
Transgenic Nicotiana Tabacum

Transgenic Nicotiana tabacum L. (tobacco) plants expressing an unregulated gene for pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase (PFP) from the fermentor protist Giardia lamblia were produced. Independently transformed lines revealed a high level of Giardia PFP activity but unaltered activities of native plant PFP, phosphofructokinase, and fructose-1,6-bisphosphatase. Transgenic plants exhibited a decrease in total biomass but no dramatic physiological or morphological alterations or significant reduction of seed yield. Both source and sink tissues demonstrated altered partitioning: leaf starch was significantly lower at the beginning and end of the daily light period and young seeds had lower starch but higher lipid levels, and these changes were correlated with PFP activity. Transgenic seeds had significantly higher percentages of germination, and microscopic examination of these seeds showed a temporal enhancement in growth of the young embryo. The significance of these results as they relate to our current understanding of PFP is discussed.Key words: pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase (PFP), transgenic, Nicotiana tabacum, glycolysis, photosynthate partitioning.

Induced expression of Serratia marcescens ribonuclease III gene in transgenic Nicotiana tabacum L. cv. SR1 tobacco plants

2016 ◽  
Vol 52 (11) ◽  
pp. 1137-1141 ◽  
Author(s):  
I. V. Zhirnov ◽  
E. A. Trifonova ◽  
A. V. Romanova ◽  
E. A. Filipenko ◽  
M. V. Sapotsky ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Serratia Marcescens ◽  
Ribonuclease Iii ◽  
Tobacco Plants ◽  
Induced Expression ◽  
Nicotiana Tabacum L ◽  
Transgenic Nicotiana Tabacum

In vivo activity of recombinant human lewis fucosyltransferase III in leaves of Nicotiana tabacum L.

2008 ◽  
Vol 52 (2) ◽  
pp. 267-274 ◽  
Author(s):  
S. Lhernould ◽  
P. Labrousse ◽  
D. Lejolly ◽  
R. Leonard ◽  
M. Carlue ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L

Lamellar lipoprotein of Nicotiana chloroplasts: in vivo incorporation of carbon dioxide, acetate, and leucine

1969 ◽  
Vol 47 (5) ◽  
pp. 667-673 ◽  
Author(s):  
Richard S. Bishop ◽  
Mary J. Perry ◽  
Richard W. Schreiber
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Nicotiana Tabacum ◽  
Gel Electrophoresis ◽  
Continuous Flow ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Acetate Incorporation ◽  
Nicotiana Tabacum L

The use of carbon dioxide, acetate, and leucine in the synthesis of lamellar lipoprotein was investigated in chloroplasts of Nicotiana tabacum L. seedlings. These 14C-labelled substrates were fed to sterile seedlings and lamellae were isolated from seedling chloroplasts. The major lamellar lipoprotein was extracted by continuous-flow polyacrylamide gel electrophoresis and the lipoprotein was separated into protein and lipid fractions.All of the substrates were incorporated into both protein and lipid. Carbon dioxide and acetate incorporation was more strongly light dependent than leucine. Light–dark and protein–lipid incorporation ratios were established for each substrate. Leucine label was found in many lamellar amino acids.

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