Acute Changes in Gill Na+‐K+‐ATPase and Creatine Kinase in Response to Salinity Changes in the Euryhaline Teleost, Tilapia (Oreochromis mossambicus)

2002 ◽  
Vol 75 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Ching‐Feng Weng ◽  
Chia‐Chang Chiang ◽  
Hong‐Yi Gong ◽  
Mark Hung‐Chih Chen ◽  
Cliff Ji‐Fan Lin ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Oreochromis Mossambicus ◽  
Salinity Changes ◽  
Euryhaline Teleost ◽  
Acute Changes

Cellular distributions of creatine kinase in branchia of euryhaline tilapia (Oreochromis mossambicus)

2003 ◽  
Vol 284 (1) ◽  
pp. C233-C241 ◽  
Author(s):  
Li-Yih Lin ◽  
Chia-Chang Chiang ◽  
Hong-Yi Gong ◽  
Ching-Yi Cheng ◽  
Pung-Pung Hwang ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Cellular Localization ◽  
Potential Role ◽  
Oreochromis Mossambicus ◽  
Staining Intensity ◽  
Muscle Type ◽  
Environmental Fluctuation ◽  
High Association ◽  
Salinity Changes

Although euryhaline teleosts can adapt to environmental fluctuation of salinity, their energy source for responding to changes in salinity and osmolarity remains unclear. This study examines the cellular localization of creatine kinase (CK) expression in branchia of tilapia ( Oreochromis mossambicus). Western blot analysis of muscle-type CK (MM form) revealed a high association with salinity changes, but BB and MB forms of CK in the gills of fish adapted to seawater did not change. With the use of immunocytochemistry, three CK isoforms (MM, MB, and BB) were localized in mitochondria-rich (MR) cells and other epithelial cells of tilapia gills. In addition, staining intensity of MM-form CK in MR cells increased after seawater transfer, whereas BB and MB forms did not significantly change. To our knowledge, this work presents the first evidence of CK expression in MR cells of tilapia gills, highlighting the potential role of CK in providing energy for ion transport.

scholarly journals Prolactin 177, prolactin 188, and extracellular osmolality independently regulate the gene expression of ion transport effectors in gill of Mozambique tilapia

2015 ◽  
Vol 309 (10) ◽  
pp. R1251-R1263 ◽  
Author(s):  
Mayu Inokuchi ◽  
Jason P. Breves ◽  
Shunsuke Moriyama ◽  
Soichi Watanabe ◽  
Toyoji Kaneko ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Incubation Time ◽  
Oreochromis Mossambicus ◽  
Mrna Levels ◽  
Ion Transporters ◽  
Local Effects ◽  
Mozambique Tilapia ◽  
Gill Filaments ◽  
Euryhaline Teleost

This study characterized the local effects of extracellular osmolality and prolactin (PRL) on branchial ionoregulatory function of a euryhaline teleost, Mozambique tilapia ( Oreochromis mossambicus). First, gill filaments were dissected from freshwater (FW)-acclimated tilapia and incubated in four different osmolalities, 280, 330, 380, and 450 mosmol/kg H2O. The mRNA expression of Na+/K+-ATPase α1a (NKA α1a) and Na+/Cl− cotransporter (NCC) showed higher expression with decreasing media osmolalities, while Na+/K+/2Cl− cotransporter 1a (NKCC1a) and PRL receptor 2 (PRLR2) mRNA levels were upregulated by increases in media osmolality. We then incubated gill filaments in media containing ovine PRL (oPRL) and native tilapia PRLs (tPRL177 and tPRL188). oPRL and the two native tPRLs showed concentration-dependent effects on NCC, NKAα1a, and PRLR1 expression; Na+/H+ exchanger 3 (NHE3) expression was increased by 24 h of incubation with tPRLs. Immunohistochemical observation showed that oPRL and both tPRLs maintained a high density of NCC- and NKA-immunoreactive ionocytes in cultured filaments. Furthermore, we found that tPRL177 and tPRL188 differentially induce expression of these ion transporters, according to incubation time. Together, these results provide evidence that ionocytes of Mozambique tilapia may function as osmoreceptors, as well as directly respond to PRL to modulate branchial ionoregulatory functions.

Control of spermatozoan motility in a euryhaline teleost acclimated to different salinities

1984 ◽  
Vol 62 (12) ◽  
pp. 2674-2677 ◽  
Author(s):  
Brian Harvey ◽  
R. Norman Kelley
Keyword(s):  
Oreochromis Mossambicus ◽  
Distilled Water ◽  
Mannitol Solution ◽  
Euryhaline Teleost ◽  
The Mean

Motility in Oreochromis mossambicus spermatozoa was determined in mannitol solutions of varying osmolality. The number of spermatozoa activated was maximal in distilled water and declined with increasing osmolality. Transfer of males from freshwater to dilute seawater (22‰) and full seawater (35‰) increased motility at any given osmolality within 2 days. Spermatozoa from fish held at three test salinities showed varying degrees of motility at the mean osmolality of undiluted milt (321 mosmol/kg). Spermatozoa were fertile in full seawater, although motility in this medium and in a mannitol solution of the same osmolality was only 0.2%. The involvement of a second factor modulating spermatozoan motility in O. mossambicus is proposed.

scholarly journals Calcium pump activities in the kidneys of Oreochromis mossambicus

1995 ◽  
Vol 198 (6) ◽  
pp. 1351-1357
Author(s):  
M Bijvelds ◽  
A Heijden ◽  
G Flik ◽  
P Verbost ◽  
Z Kolar ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Fraction ◽  
Transport Mechanism ◽  
Physiological Role ◽  
Membrane Vesicles ◽  
Oreochromis Mossambicus ◽  
High Affinity ◽  
Pump Activity ◽  
Basolateral Plasma Membrane ◽  
Euryhaline Teleost

The mechanism that underlies transcellular Ca2+ reabsorption in the kidney of the euryhaline teleost Oreochromis mossambicus was studied. Preparations of membrane vesicles made from the kidneys of freshwater- and seawater-adapted fish were more than sevenfold enriched in the basolateral plasma membrane marker Na+/K+-ATPase. Significant recovery of NADH­ cytochrome c reductase enzyme activity and of oxalate-stimulated Ca2+ pump activities in the membrane preparations indicated that the membrane fraction was of endoplasmic reticular origin. Indeed, thapsigargin specifically inhibited Ca2+ pump activity that could be attributed to oxalate-permeable endoplasmic reticular fragments. Kinetic analysis of thapsigargin-insensitive Ca2+ pump activity indicated the existence of a homogeneous, high-affinity, ATP-driven Ca2+ pump. No Na+-driven Ca2+ transport mechanism could be demonstrated. Plasma membrane Ca2+ pump activity was 56 % lower in preparations from seawater-adapted fish than in preparations from freshwater-adapted fish, suggesting a physiological role for this Ca2+ pump activity in renal Ca2+ handling by euryhaline species, with an involvement in the regulation of Ca2+ reabsorption.

Hormone release is tied to changes in cell size in the osmoreceptive prolactin cell of a euryhaline teleost fish, the tilapia, Oreochromis mossambicus

2004 ◽  
Vol 138 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Gregory M Weber ◽  
Andre P Seale ◽  
N.Harold Richman III ◽  
M.H Stetson ◽  
Tetsuya Hirano ◽  
...  
Keyword(s):  
Cell Size ◽  
Teleost Fish ◽  
Oreochromis Mossambicus ◽  
Hormone Release ◽  
Prolactin Cell ◽  
Euryhaline Teleost
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