In vitro Culture of Western Redcedar (Thuja plicata Donn). I. Plantlet Formation

1977 ◽  
Vol 138 (3) ◽  
pp. 298-304 ◽  
Author(s):  
Warren K. Coleman ◽  
Trevor A. Thorpe
Keyword(s):  
In Vitro Culture ◽  
Thuja Plicata ◽  
Western Redcedar ◽  
Plantlet Formation ◽  
Thuja Plicata Donn

Effects of leaching on fungal growth and decay of western redcedar

2009 ◽  
Vol 55 (5) ◽  
pp. 578-586 ◽  
Author(s):  
Russell J. Chedgy ◽  
Young Woon Lim ◽  
Colette Breuil
Keyword(s):  
Fungal Growth ◽  
Fungal Species ◽  
Heterobasidion Annosum ◽  
Thuja Plicata ◽  
Western Redcedar ◽  
Coniophora Puteana ◽  
Soil Block ◽  
Block Test ◽  
Thuja Plicata Donn

We tested the effect of leaching on the concentration of western redcedar (WRC; Thuja plicata Donn ex D. Don) heartwood extractives that are known to exhibit antimicrobial activity and correlated this with fungal growth and decay. We assessed the extractive tolerance of the following fungal species: Acanthophysium lividocaeruleum , Coniophora puteana , Heterobasidion annosum , Pachnocybe ferruginea , Phellinus sulphurascens , and Phellinus weirii by measuring their growth rate (mm/day) on media with or without WRC leachate. These data were correlated with the ability of the fungal species to grow on and decay leached versus nonleached WRC. We used an ergosterol assay to estimate growth and a standard soil-block test to assess decay. We estimated that leaching reduced the concentration of 5 major extractives: (–)-plicatic acid, β-thujaplicin, γ-thujaplicin, β-thujaplicinol, and thujic acid by ~80%. Phellinus sulphurascens exhibited the lowest extractive-tolerance in vitro, grew poorly on and caused minimal decay in nonleached WRC, but it grew well on and decayed pine and leached WRC. Coniophora puteana, H. annosum, and P. weirii displayed moderate to high tolerance to leachate, grew on and caused decay in nonleached as well as leached WRC, but their growth and decay were always greatest on leached WRC and pine, suggesting that leaching enhances decay by these fungi. Acanthophysium lividocaeruleum and Pachnocybe ferruginea exhibited high extractive-tolerance. Whereas A. lividocaeruleum clearly caused decay on all types of wood, no decay was observed with Pachnocybe ferruginea, which grew very slowly in the different wood species, and it may or may not be able to decay wood.

FILBERT PLANTLET FORMATION THROUGH "IN VITRO" CULTURE

1987 ◽  
pp. 505-510 ◽  
Author(s):  
C. Pérez ◽  
A. Rodríguez ◽  
A. Revilla ◽  
R. Rodríguez ◽  
R. Sánchez-Tamés
Keyword(s):  
In Vitro Culture ◽  
Plantlet Formation

scholarly journals Effect of Foliar-Applied Gibberellin A3 on Male and Female Strobilus Production and Cone and Seed Quality in Western Redcedar (Thuja plicata Donn)

2007 ◽  
Vol 22 (4) ◽  
pp. 297-306
Author(s):  
J.H. Russell ◽  
O. Hak
Keyword(s):  
Seed Quality ◽  
Foliar Application ◽  
Seed Orchard ◽  
Shoot Elongation ◽  
Thuja Plicata ◽  
Western Redcedar ◽  
Male And Female ◽  
Cone Production ◽  
Gibberellin A3 ◽  
Thuja Plicata Donn

Abstract Western redcedar (Thuja plicata Donn) foliar-applied gibberellin A3 (GA3) induction trials were performed over a 4-year period at a number of different seed orchards in coastal British Columbia. The effects of GA3 timing, concentration, and frequency on male and female strobilus production, as well as timing on seed quality, were studied. Male and female strobili were induced over the complete span of shoot elongation from May to August, indicative of a less-precise induction period than species in the Pinaceae family. Female strobilus production was correlated with shoot increment, such that maximum cone production was associated with maximum shoot elongation. In addition, seed quality decreased with decreasing shoot increments in August. For operational efficiency, a one-time foliar application of 200 mg/l GA3 is sufficient for adequate female strobilus production. To increase the female-to-male strobilus ratio, a two-time foliar application of GA3 mid-May and mid-July, concentrating on vigorous shoots, is recommended. Results are discussed in relation to seed orchard management techniques that may potentially influence selfing rates.

scholarly journals Adventitious Shoot and Plantlet Formation from Cultured Apple Leaf Explants

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629f-629
Author(s):  
Karim H. Al-Juboory ◽  
Jabar H. Al-Niami
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Malus Domestica ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Plantlet Formation ◽  
Petri Dishes

Leaves of wild apple (Malus domestica Borkh) were excised from in vitro grown shoots transversely cut into halves and plated onto petri dishes containing regeneration media. Cultures were kept in the dark for three weeks before adventitious shoots were observed. Callus from leaf explants produced adventitious shoots after 3 months of in vitro culture. Callus were cultured on Nitsch and Nitsch medium supplemented with a range of BA (0.0–2.0 μm) and NAA (0.0–10 μm). BA at 10 μm combined with NAA (0.5 μm) proved most effective for stimulating shoot proliferation of cultured apple. Plantlets from tissue culture were easily transferred to the greenhouse environment.

Patterns of post-glacial colonization by western redcedar (Thuja plicata, Cupressaceae) as revealed by microsatellite markers

Botany ◽  
2008 ◽  
Vol 86 (2) ◽  
pp. 194-203 ◽  
Author(s):  
Lisa M. O’Connell ◽  
Kermit Ritland ◽  
Stacey Lee Thompson
Keyword(s):  
Genetic Distances ◽  
Thuja Plicata ◽  
Strongly Correlated ◽  
Dynamic Nature ◽  
Western Redcedar ◽  
Inbreeding Coefficients ◽  
Expected Heterozygosity ◽  
Local Extinctions ◽  
Insight Into ◽  
Thuja Plicata Donn

As knowledge of historical migration in response to climatic change allows insight into the dynamic nature of range shifts, patterns of post-glacial colonization were evaluated for the western redcedar ( Thuja plicata Donn ex D. Don). We sampled and genotyped 620 trees from 23 populations across its range, including disjunct coastal and interior mesic sites. Genetic variation at eight microsatellite loci (mean alleles/locus = 10.30, mean expected heterozygosity = 0.755) was much higher than previous studies involving other markers, and inbreeding coefficients were predominantly positive (mean = 0.110). The two southernmost populations showed greatest genetic distances, while remaining populations clustered into three distinct geographic groups, comprising northern–coastal, central, and southern–interior populations, respectively. Genetic diversity decreased with latitude, while genetic and geographic distances were strongly correlated (r = 0.788). Our findings are consistent with independent routes of relatively recent colonization from one major refugium, located south of the glacial maximum, rather than ancient vicariant events. Regional bottlenecks, detected in the south of the range, may have resulted from local extinctions as the range of western redcedar advanced northward. Combined with inbreeding and the evolution of inbreeding tolerance, this may have promoted homozygosity for most classes of genetic markers as observed in other studies of this species.

The mating system in natural populations of western redcedar (Thuja plicata)

2001 ◽  
Vol 79 (6) ◽  
pp. 753-756 ◽  
Author(s):  
Lisa M O'Connell ◽  
Frédérique Viard ◽  
John Russell ◽  
Kermit Ritland
Keyword(s):  
British Columbia ◽  
Mating System ◽  
Natural Populations ◽  
Seed Orchard ◽  
Outcrossing Rate ◽  
Thuja Plicata ◽  
Western Redcedar ◽  
Outcrossing Rates ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Thuja Plicata Donn

Outcrossing rates and the correlation of paternity were estimated in six natural populations of western redcedar (Thuja plicata Donn. ex D. Don) in southwestern British Columbia. Over 3000 offspring were assayed, as progeny arrays, for the only sufficiently polymorphic isozyme locus in this species, glucose-6-phosphate dehydrogenase. Estimates of population outcrossing rates ranged from 0.173 to 1.257 and averaged 0.715 ± 0.045 (mean ± SD). Estimates of the correlation of paternity generally did not differ from zero. Six of the seven outcrossing estimates (one population was surveyed in two consecutive years) were higher than a previous seed orchard study. However, these outcrossing rates are still lower than those estimated for most other species of conifers. Population outcrossing rates also showed wide variation, and this variation is discussed in terms of ecological and phenological differences among populations.Key words: Cupressaceae, conifers, isozymes, outcrossing rate, inbreeding.

scholarly journals Deterioration of western redcedar (Thuja plicata Donn ex D. Don) seeds: protein oxidation and in vivo NMR monitoring of storage oils

2008 ◽  
Vol 59 (4) ◽  
pp. 765-777 ◽  
Author(s):  
Victor V. Terskikh ◽  
Ying Zeng ◽  
J. Allan Feurtado ◽  
Michael Giblin ◽  
Suzanne R. Abrams ◽  
...  
Keyword(s):  
Protein Oxidation ◽  
Thuja Plicata ◽  
Western Redcedar ◽  
In Vivo Nmr ◽  
Thuja Plicata Donn

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens
Sign in / Sign up

Export Citation Format

Share Document