In vitro Physiology and Morphogenesis of Cheiranthus cheiri var. Clott of Gold and C. cheiri var. Goliath

1970 ◽  
Vol 131 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Pushpa Khanna ◽  
E. John Staba
Keyword(s):  
Cheiranthus Cheiri ◽  
In Vitro Physiology

scholarly journals Tissue-Engineered Large Intestine Resembles Native Colon With Appropriate In Vitro Physiology and Architecture

2003 ◽  
Vol 238 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Tracy C. Grikscheit ◽  
Erin R Ochoa ◽  
Anthony Ramsanahie ◽  
Eben Alsberg ◽  
David Mooney ◽  
...  
Keyword(s):  
Large Intestine ◽  
In Vitro Physiology

scholarly journals Guanosine 3',5'-cyclic monophosphate and the in vitro physiology of frog photoreceptor membranes.

1977 ◽  
Vol 69 (5) ◽  
pp. 667-679 ◽  
Author(s):  
M L Woodruff ◽  
D Bownds ◽  
S H Green ◽  
J L Morrisey ◽  
A Shedlovsky
Keyword(s):  
Plasma Membrane ◽  
Light Intensity ◽  
Cyclic Gmp ◽  
Outer Segment ◽  
Phosphodiesterase Inhibitor ◽  
Plasma Membrane Permeability ◽  
Cyclic Monophosphate ◽  
Outer Segments ◽  
In Vitro Physiology

Frog rod outer segments freshly detached from dark-adapted retinas contain approximately 1-2 molecules of guanosine 3',5'-cyclic monophosphate (cyclic GMP) for every 100 molecules of visual pigment present. This cyclic GMP decays to 5'-GMP, and the conversion is accelerated upon illumination of the outer segments. Bleaching one rhodopsin molecule can lead to the hydrolysis of 1,000-2,000 molecules of cyclic GMP within 100-300 ms. The decline in cyclic GMP concentration becomes larger as illumination increases, and varies with the logarithm of light intensity at levels which bleach between 5 X 10(2) and 5 X 10(5) rhodopsin molecules per outer segment-second. Light suppression of plasma membrane permeability, assayed in vitro as light suppression of outer segment swelling in a modified Ringer's solution, occurs over this same range of light intensity. The correlation between cyclic GMP and permeability or swelling is maintained in the presence of two pharmacological perturbations: papaverine, a phosphodiesterase inhibitor, increases both cyclic GMP levels and the dark permeability of the plasma membrane; and beta,gamma-methylene ATP increases the effectiveness of light in suppressing both permeability and cyclic GMP levels.

scholarly journals Imaging light responses of retinal ganglion cells in the living mouse eye

2013 ◽  
Vol 109 (9) ◽  
pp. 2415-2421 ◽  
Author(s):  
Lu Yin ◽  
Ying Geng ◽  
Fumitaka Osakada ◽  
Robin Sharma ◽  
Ali H. Cetin ◽  
...  
Keyword(s):  
Retinal Ganglion Cells ◽  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Ganglion Cells ◽  
Morphological Development ◽  
Retinal Ganglion ◽  
Calcium Indicator ◽  
In Vitro Physiology

This study reports development of a novel method for high-resolution in vivo imaging of the function of individual mouse retinal ganglion cells (RGCs) that overcomes many limitations of available methods for recording RGC physiology. The technique combines insertion of a genetically encoded calcium indicator into RGCs with imaging of calcium responses over many days with FACILE (functional adaptive optics cellular imaging in the living eye). FACILE extends the most common method for RGC physiology, in vitro physiology, by allowing repeated imaging of the function of each cell over many sessions and by avoiding damage to the retina during removal from the eye. This makes it possible to track changes in the response of individual cells during morphological development or degeneration. FACILE also overcomes limitations of existing in vivo imaging methods, providing fine spatial and temporal detail, structure-function comparison, and simultaneous analysis of multiple cells.

Influence of Mistletoe Extracts and Its Components on in Vitro Physiology of Cancer Cells

2005 ◽  
pp. 183-185
Author(s):  
F. Sidler ◽  
T. Wermelinger ◽  
L. Rist ◽  
A. Hensel ◽  
A. Viviani
Keyword(s):  
Cancer Cells ◽  
Mistletoe Extracts ◽  
In Vitro Physiology

Epileptiform discharges in the human dysplastic neocortex: In vitro physiology and pharmacology

1999 ◽  
Vol 46 (6) ◽  
pp. 816-826 ◽  
Author(s):  
Massimo Avoli ◽  
Andrea Bernasconi ◽  
Donatella Mattia ◽  
Andr� Olivier ◽  
Granger G. C. Hwa
Keyword(s):  
Epileptiform Discharges ◽  
In Vitro Physiology
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