scholarly journals Measuring and Interpreting Associations between Antibiotic Use and Penicillin Resistance in Streptococcus pneumoniae

2001 ◽  
Vol 32 (7) ◽  
pp. 1044-1054 ◽  
Author(s):  
M. Lipsitch
Keyword(s):  
Streptococcus Pneumoniae ◽  
Antibiotic Use ◽  
Penicillin Resistance

Quintuplex PCR to Detect Antibiotic Resistance Genes in Streptococcus pneumoniae

2016 ◽  
Vol 1 (2) ◽  
pp. 22 ◽  
Author(s):  
Navindra Kumari Palanisamy ◽  
Parasakthi Navaratnam ◽  
Shamala Devi Sekaran
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Bacterial Pathogen ◽  
Antibiotic Resistance Genes ◽  
Pcr Amplification ◽  
Penicillin Resistance ◽  
Penicillin Binding Proteins ◽  
Efflux Mechanism ◽  
Mic Value

Introduction: Streptococcus pneumoniae is an important bacterial pathogen, causing respiratory infection. Penicillin resistance in S. pneumoniae is associated with alterations in the penicillin binding proteins, while resistance to macrolides is conferred either by the modification of the ribosomal target site or efflux mechanism. This study aimed to characterize S. pneumoniae and its antibiotic resistance genes using 2 sets of multiplex PCRs. Methods: A quintuplex and triplex PCR was used to characterize the pbp1A, ermB, gyrA, ply, and the mefE genes. Fifty-eight penicillin sensitive strains (PSSP), 36 penicillin intermediate strains (PISP) and 26 penicillin resistance strains (PRSP) were used. Results: Alteration in pbp1A was only observed in PISP and PRSP strains, while PCR amplification of the ermB or mefE was observed only in strains with reduced susceptibility to erythromycin. The assay was found to be sensitive as simulated blood cultures showed the lowest level of detection to be 10cfu. Conclusions: As predicted, the assay was able to differentiate penicillin susceptible from the non-susceptible strains based on the detection of the pbp1A gene, which correlated with the MIC value of the strains.

scholarly journals Determination of penicillin resistance in Streptococcus pneumoniae isolates from southern Brazil by PCR

2006 ◽  
Vol 10 (2) ◽  
pp. 110-115 ◽  
Author(s):  
Eduardo Walker Zettler ◽  
Rosane Machado Scheibe ◽  
Cícero A.G. Dias ◽  
Patrícia Santafé ◽  
Diógenes Santiago Santos ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Penicillin Resistance ◽  
Southern Brazil

scholarly journals Bacteremic Pneumonia Caused by Penicillin-Resistant Pneumococci: Case Report and Review with a Canadian Perspective

1992 ◽  
Vol 3 (4) ◽  
pp. 185-188
Author(s):  
David R Burdge ◽  
Vincent C Woo ◽  
Patricia MA Ritchie
Keyword(s):  
Antibiotic Resistance ◽  
Case Report ◽  
Streptococcus Pneumoniae ◽  
Minimal Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Penicillin Resistance ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Canadian Perspective ◽  
Bacteremic Pneumonia

A Canadian adult with bacteremic pneumonia caused by a relatively penicillin-resistant (minimal inhibitory concentration 0.25 μg/mL) Streptococcus pneumoniae is reported, and the published literature regarding penicillin-resistant pneumococci in Canada reviewed. Although penicillin resistance has been reported infrequently to date, this case emphasizes the need for routine antimicrobial sensitivity testing of all pneumococci isolated from normally sterile sites, and for ongoing systematic surveillance for penicillin and other antibiotic resistance in Canada.

scholarly journals Capsule Homology Does Not Increase the Frequency of Transformation of Linked Penicillin Binding Proteins PBP 1a and PBP 2x in Streptococcus pneumoniae

2005 ◽  
Vol 49 (4) ◽  
pp. 1591-1592 ◽  
Author(s):  
Krzysztof Trzciński ◽  
Adam MacNeil ◽  
Keith P. Klugman ◽  
Marc Lipsitch
Keyword(s):  
Streptococcus Pneumoniae ◽  
Binding Proteins ◽  
Penicillin Resistance ◽  
Penicillin Binding Proteins ◽  
Transformation Rates

ABSTRACT Penicillin resistance is mainly confined to a limited number of Streptococcus pneumoniae serotypes. Given linkage between the capsular biosynthesis locus and two penicillin binding proteins, we tested whether capsule homology increases transformation rates of penicillin resistance. Transformation rates in homologous donor-recipient pairs were no higher than expected, falsifying this hypothesis.

Gonorrhoea in a south London genitourinary medicine department

2003 ◽  
Vol 14 (9) ◽  
pp. 625-629 ◽  
Author(s):  
A Newell ◽  
E Herbert ◽  
J Vigus ◽  
A Grieg ◽  
M E Rodgers MRCOG
Keyword(s):  
Demographic Data ◽  
Antibiotic Use ◽  
Penicillin Resistance ◽  
Heterosexual Men ◽  
Homosexual Men ◽  
National Guidelines ◽  
Medicine Department ◽  
Direct Microscopy ◽  
Medicine Clinic ◽  
Genitourinary Medicine

The management and outcome of all cases of gonorrhoea which presented to a south London genitourinary medicine clinic during 1999 were assessed and compared with published national guidelines. The incidence of penicillin resistance was calculated, as was the rate of co-infection with chlamydia and trichomonas. Information regarding demographic data, microscopy, culture results, test of cure, antibiotic use, sensitivity and health adviser contact was examined. A total of 257 cases of gonorrhoea were diagnosed in 238 patients. Heterosexual men constituted 52.9% of cases, 6.6% were in homosexual men and 40.5% in women. Direct microscopy was positive in 88.8% of men and in 40.5% of women. In women, the rate of gonorrhoea co-infection with chlamydia was 34.7% and with trichomonas was 11.5%. In men the rate of chlamydia co-infection was only 3.3%, however, we do not believe this to be an accurate figure as we are unable to routinely screen all men for chlamydia due to financial restrictions. Amoxicillin with probenecid were the most commonly used antibiotics in line with local guidelines. Penicillin resistance was demonstrated in 4.6% of infected cases. Health advisers saw 73.2% of patients.

Sign in / Sign up

Export Citation Format

Share Document