scholarly journals Small Evolutionarily Conserved RNA, Resembling C/D Box Small Nucleolar RNA, Is Transcribed from PWCR1, a Novel Imprinted Gene in the Prader-Willi Deletion Region, Which Is Highly Expressed in Brain

2000 ◽  
Vol 67 (5) ◽  
pp. 1067-1082 ◽  
Author(s):  
Tala de los Santos ◽  
Johannes Schweizer ◽  
Christian A. Rees ◽  
Uta Francke
Keyword(s):  
Small Nucleolar Rna ◽  
Imprinted Gene ◽  
Deletion Region ◽  
Evolutionarily Conserved ◽  
Nucleolar Rna

scholarly journals Multiple conserved segments of E1 small nucleolar RNA are involved in the formation of a ribonucleoprotein particle in frog oocytes

2000 ◽  
Vol 348 (3) ◽  
pp. 517-524 ◽  
Author(s):  
Donald D. RUHL ◽  
Mary Ellen PUSATERI ◽  
George L. ELICEIRI
Keyword(s):  
Gel Electrophoresis ◽  
Nucleolar Localization ◽  
Nucleotide Substitutions ◽  
Small Nucleolar Rna ◽  
Conserved Sequences ◽  
Exogenous Rna ◽  
Evolutionarily Conserved ◽  
Substitution Mutations ◽  
The Stability ◽  
Nucleolar Rna

E1/U17 small nucleolar RNA (snoRNA) is a box H/ACA snoRNA. To identify E1 RNA elements required for its assembly into a ribonucleoprotein (RNP) particle, we have made substitution mutations in evolutionarily conserved sequences and structures of frog E1 RNA. After E1 RNA was injected into the nucleus of frog oocytes, assembly of this exogenous RNA into an RNP was monitored by non-denaturing gel electrophoresis. Unexpectedly, nucleotide substitutions in many phylogenetically conserved segments of E1 RNA produced RNPs with abnormal gel-electrophoresis patterns. These RNA segments were at least nine conserved sequences and an apparently conserved structure. In another region needed for RNP formation, the requirement may be sequence(s) and/or structure. Base substitutions in each of these and in one additional conserved E1 RNA segment reduced the stability of this snoRNA in frog oocytes. Nucleolar localization was assayed by fluorescence microscopy after injection of fluorescein-labelled RNA. The H box (ANANNA) and the ACA box are both needed for efficient nucleolar localization of frog E1 RNA.

The multifunctional RNase XRN2

2013 ◽  
Vol 41 (4) ◽  
pp. 825-830 ◽  
Author(s):  
Takashi S. Miki ◽  
Helge Großhans
Keyword(s):  
Small Nucleolar Rna ◽  
Cellular Processes ◽  
Evolutionarily Conserved ◽  
Mrna Precursor ◽  
Nucleolar Rna ◽  
Precursor Mrna

Different classes of RNA function in various cellular processes, and their biogenesis and turnover involve diverse RNases for processing and degradation. XRN2 is a 5′→3′ exoribonuclease that is evolutionarily conserved in eukaryotes. It is predominantly localized in the nucleus and recognizes single-stranded RNA with a 5′-terminal monophosphate to degrade it processively to mononucleotides. In the present paper, we review functions of XRN2 and its cofactors in maturation, surveillance and activity control of several classes of RNA such as pre-mRNA (precursor mRNA), rRNA and snoRNA (small nucleolar RNA).

Small nucleolar RNA host genes promoting epithelial–mesenchymal transition lead cancer progression and metastasis

IUBMB Life ◽  
2021 ◽  
Author(s):  
Alessio Biagioni ◽  
Shima Tavakol ◽  
Nooshin Ahmadirad ◽  
Masoumeh Zahmatkeshan ◽  
Lucia Magnelli ◽  
...  
Keyword(s):  
Cancer Progression ◽  
Epithelial Mesenchymal Transition ◽  
Small Nucleolar Rna ◽  
Mesenchymal Transition ◽  
Nucleolar Rna ◽  
Host Genes

Distribution of U3 small nucleolar RNA and fibrillarin during early embryogenesis in Caenorhabditis elegans

Biochimie ◽  
2008 ◽  
Vol 90 (6) ◽  
pp. 898-907 ◽  
Author(s):  
Yumi Sasano ◽  
Yusuke Hokii ◽  
Kunio Inoue ◽  
Hiroshi Sakamoto ◽  
Chisato Ushida ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Early Embryogenesis ◽  
Small Nucleolar Rna ◽  
Nucleolar Rna

scholarly journals Upregulation of Long Non-Coding RNA Small Nucleolar RNA Host Gene 12 Contributes to Cell Growth and Invasion in Cervical Cancer by Acting as a Sponge for MiR-424-5p

2018 ◽  
Vol 45 (5) ◽  
pp. 2086-2094 ◽  
Author(s):  
Jing Dong ◽  
Qing Wang ◽  
Li Li ◽  
Zhang Xiao-jin
Keyword(s):  
Cervical Cancer ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Host Gene ◽  
Migration And Invasion ◽  
Small Nucleolar Rna ◽  
Cervical Cancer Cells ◽  
Cancer Tissues ◽  
Nucleolar Rna

Background/Aims: Cervical cancer, which is one of the most aggressive cancers affecting females, has high rates of recurrence and mortality. Small nucleolar RNA host gene 12 (SNHG12) is known to promote the progression of several cancers; however, its exact effects and molecular mechanisms in cervical cancer remain unknown. Methods: Real-time quantitative PCR was used to determine the expression level of SNHG12 in cervical cancer tissues and cell lines. Loss-of-function assays were performed to examine the effect of SNHG12 on the proliferation, apoptosis, migration and invasion of cervical cancer cells in vitro and tumor growth in vivo. Luciferase experiments were employed to explore the interactions between SNHG12 and miR-424-5p. Results: SNHG12 was found to be abnormally elevated in human cervical cancer tissues compared with paired adjacent normal tissues. Moreover, high SNHG12 expression in tumor tissues was significantly correlated with vascular involvement, lymph node metastasis, advanced FIGO stage and poor prognosis. Furthermore, the knockdown of SNHG12 was found to inhibit proliferation, migration and invasion of cervical cancer cells in vitro, and silencing SNHG12 was shown to suppress tumor growth in a nude mouse model. Mechanistic studies showed that SNHG12 functioned as an endogenous sponge for miR-424-5p, thereby downregulating the expression of miR-424-5p in cervical cancer. Furthermore, the inhibition of miR-424-5p in SNHG12-depleted cells partially reversed the effects on cervical cancer cell apoptosis, adhesion and invasion. Conclusion: In summary, our findings suggest that the tumor-promoting role of SNHG12 is to function as a molecular sponge, which negatively regulates miR-424-5p. These findings may provide a potent therapeutic target for cervical cancer.

Sign in / Sign up

Export Citation Format

Share Document