scholarly journals Isolation and Chromosomal Localization of a Cornea-Specific Human Keratin 12 Gene and Detection of Four Mutations in Meesmann Corneal Epithelial Dystrophy

1997 ◽  
Vol 61 (6) ◽  
pp. 1268-1275 ◽  
Author(s):  
Kohji Nishida ◽  
Yoichi Honma ◽  
Atsuyoshi Dota ◽  
Satoshi Kawasaki ◽  
Wakako Adachi ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Corneal Epithelial ◽  
Keratin 12

scholarly journals Study of corneal epithelial progenitor origin and the Yap1 requirement using keratin 12 lineage tracing transgenic mice

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ramesh Babu Kasetti ◽  
Subhash Gaddipati ◽  
Shifu Tian ◽  
Lei Xue ◽  
Winston W.-Y. Kao ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Lineage Tracing ◽  
Corneal Epithelial ◽  
Keratin 12

scholarly journals A Case of Solitary Nonvascularized Corneal Epithelial Dysplasia

2016 ◽  
Vol 2016 ◽  
pp. 1-4
Author(s):  
Tomoya Morii ◽  
Takayoshi Sumioka ◽  
Ai Izutani-Kitano ◽  
Yukihisa Takada ◽  
Yuka Okada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Corneal Epithelium ◽  
Ocular Surface ◽  
Histopathological Examination ◽  
Precancerous Lesion ◽  
Intraepithelial Neoplasia ◽  
Epithelial Dysplasia ◽  
Corneal Epithelial ◽  
Keratin 13 ◽  
Keratin 12

Background. Epithelial dysplasia is categorized as conjunctival/corneal intraepithelial neoplasia which is a precancerous lesion. The lesion is usually developed at the limbal region and grows towards central cornea in association with neovascularization into the lesion. Here, we report a case of isolated nonvascularized corneal epithelial dysplasia surrounded by normal corneal epithelium with immune histochemical finding of ocular surface tissues cytokeratins, for example, keratin 13 and keratin 12.Case Presentation. A 76-year-old man consulted us for visual disturbance with localized opacification of the corneal epithelium in his left eye. His visual acuity was 20/20 and 20/200 in his right and left eye, respectively. Slit lamp examination showed a whitish plaque-like lesion at the center of his left corneal epithelium. No vascular invasion to the lesion was found. The lesion was surgically removed and subjected to histopathological examination and diagnosed as epithelial dysplasia. Amyloidosis was excluded by direct fast scarlet 4BS (DFS) staining. Immunohistochemistry showed that the dysplastic epithelial cells express keratin 13 and vimentin, but not keratin 12, indicating that the neoplastic epithelial cells lacked corneal-type epithelium differentiation.Conclusions. The lesion was diagnosed as nonvascularized epithelial dysplasia of ocular surface. Etiology of the lesion is not known.

scholarly journals Conditional Deletion of AP-2β in the Periocular Mesenchyme of Mice Alters Corneal Epithelial Cell Fate and Stratification

2021 ◽  
Vol 22 (16) ◽  
pp. 8730
Author(s):  
Haydn Walker ◽  
Aftab Taiyab ◽  
Paula Deschamps ◽  
Trevor Williams ◽  
Judith A. West-Mays
Keyword(s):  
Cell Fate ◽  
Corneal Endothelium ◽  
Anterior Segment ◽  
Corneal Epithelial Cell ◽  
Surface Ectoderm ◽  
Corneal Epithelial ◽  
Phenotypic Changes ◽  
Conditional Deletion ◽  
Bmp4 Expression ◽  
Keratin 12

The cornea is an anterior eye structure specialized for vision. The corneal endothelium and stroma are derived from the periocular mesenchyme (POM), which originates from neural crest cells (NCCs), while the stratified corneal epithelium develops from the surface ectoderm. Activating protein-2β (AP-2β) is highly expressed in the POM and important for anterior segment development. Using a mouse model in which AP-2β is conditionally deleted in the NCCs (AP-2β NCC KO), we investigated resulting corneal epithelial abnormalities. Through PAS and IHC staining, we observed structural and phenotypic changes to the epithelium associated with AP-2β deletion. In addition to failure of the mutant epithelium to stratify, we also observed that Keratin-12, a marker of the differentiated epithelium, was absent, and Keratin-15, a limbal and conjunctival marker, was expanded across the central epithelium. Transcription factors PAX6 and P63 were not observed to be differentially expressed between WT and mutant. However, growth factor BMP4 was suppressed in the mutant epithelium. Given the non-NCC origin of the epithelium, we hypothesize that the abnormalities in the AP-2β NCC KO mouse result from changes to regulatory signaling from the POM-derived stroma. Our findings suggest that stromal pathways such as Wnt/β-Catenin signaling may regulate BMP4 expression, which influences cell fate and stratification.

Cloning, functional expression, and chromosomal localization of the human pancreatic islet glucose-dependent insulinotropic polypeptide receptor

Diabetes ◽  
1995 ◽  
Vol 44 (10) ◽  
pp. 1202-1208 ◽  
Author(s):  
S. Gremlich ◽  
A. Porret ◽  
E. H. Hani ◽  
D. Cherif ◽  
N. Vionnet ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Chromosomal Localization ◽  
Functional Expression ◽  
Human Pancreatic Islet

The effect of PRK on corneal epithelium thickness and pachmetry in treatment of myopia.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Furkaan Majied Hamied ◽  
Deyaa Neama Kadhim ◽  
Sohaib A Mahmood
Keyword(s):  
Refractive Error ◽  
Anterior Segment ◽  
Thickness Change ◽  
Corneal Epithelial ◽  
Epithelial Thickness ◽  
Refractive Outcomes ◽  
Epithelium Thickness ◽  
Profile Changes ◽  
Corneal Epithelial Thickness ◽  
Myopic Astigmatism

In order to facilitate the corneal stromal ablation in photorefractive keratectomy the epithelium is removed so corneal repair associated with changes in epithelium and stroma. To study the corneal epithelial thickness and pachymetry profile changes after photorefractive keratec­tomy (PRK) for myopia. Retrospective analysis of the postoperative corneal epithelial thickness and pachymetry profile changes in 22 eyes of 12 patients treated with PRK for myopia or myopic astigmatism. Corneal and epithelial thickness maps within the central 6 mm were obtained by anterior segment spectral-domain optical coherence tomography (SD-OCT) preop­eratively and at 3 months postoperatively. Correlations between pachymetry,epithelial thickness changes and the amount of correction,were analyzed.Compared to preoperative values,the central 2 mm and the paracentral 2 to 5 mm zone epithelium was 1 ± 2.85 and 1 ± 3.11 μm thicker,respec­tively,at 3 months postoperatively (P <.05). The spheri­cal equivalent (SE) changed from-2.80 ± 2.028 diop­ters (D) preoperatively to -0.40 ± 0.42 D at 3 months postoperatively. Females show greater postoperative epithelial thickening, 2.6 ± 3.77 μm,than males,0.34 ± 1.98 μm. There was a trend toward greater epithelial thickening with a larger amount of programmed SE correction, and thinner preoperative epithelium. No correlation between epithelial thickness change and postoperative change in refraction was detected.Negative correlation between between age, refractive error,with the pre and post-operative pachymetry. In general female pachymetry reading is higher than it in male.The corneal epithelial thickness in­creased after PRK up to 3 months postoperatively. It was affected by the amount of myopia treated, gender, and preoperative epithelial thickness. The refractive outcomes did not affected by the postoperative epithelial thickening. Negative correlation between between age, refractive error, with the pre and post-operative pachymetry. In general female pachymetry reading is higher than it in male.

Managing Corneal Epithelial Defects Using High Molecular Weight Fluorescein to Prevent Contact Lens Staining and Removal of Bandage Contact Lenses

2020 ◽  
Vol 3 (8) ◽  
pp. 20-22
Author(s):  
Muhammad Hamza ◽  
Chrishan Gunasekera ◽  
Samar Nahas ◽  
Z CX Lin ◽  
Hatch Mukherjee ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Contact Lens ◽  
Contact Lenses ◽  
Corneal Epithelial

Comparison of Ocular Surface Cytological Appearance in Glaucoma Patient Treated with Timolol Maleat 0,5% Latanoprost 0,005% and Timolol-Latanoprost Fixed Combination Preservative Free Eye Drop

2019 ◽  
Vol 44 (2) ◽  
pp. 82
Author(s):  
Maretha Amrayni ◽  
Elsa Gustianty ◽  
Susi Heryati ◽  
Andika Prahasta ◽  
Maula Rifada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Density ◽  
Goblet Cell ◽  
Ocular Surface ◽  
Fixed Combination ◽  
Cell Contact ◽  
Corneal Epithelial ◽  
Preservative Free ◽  
Goblet Cell Density ◽  
Epithelial Metaplasia

Introduction : The longterm use of topical antiglaucoma might cause ocular surface instability due to active substance or preservative used. Impression cytology examination may reveal superficial epithelial cells on conjunctiva and cornea, including goblet cells. Goblet cell density decrease is the most important parameter on evaluation of ocular surface disorder. Objective : This study was to understand ocular surface remodeling due to active substance of topical antiglaucoma with impression cytology examination among the patient prior and 3 months after therapy. Methods : This was a randomized controlled trial study with single blind masking. A total of 45 eyes from 31 patients were used as subject and distributed onto three groups treatment, which were timolol maleat 0.5%, latanoprost 0.005%, and latanoprost-timolol maleat fixed combination. All topical antiglaucoma in this study were preservative free. Result : There were differences between 3 groups in goblet cells density after 3 months therapy (p=0,030). Goblet cell density in timolol group was lower than latanoprost (p=0,041) and fixed combination (p=0,045). There was no significantly difference between 3 groups in conjunctival epithelial metaplasia degree (p=0,706) and cell to cell contact degree in corneal epithelial cells (p=0.66) after 3 months therapy. Conjunctival epithelial metaplasia degree were increased among group of timolol (p=0,008) and fixed combination (p=0,046). Conclusion : Timolol maleat 0,5% caused lower goblet cell density after 3 months therapy compare with latanoprost and fixed combination. There was no significantly difference in conjunctival epithelial metaplasia and cell to cell contact degree in corneal epithelial cells among these glaucoma treatment groups.

Chromosomal Localization of the Gene for Resistance to Powdery Mildew in the Wheat Cultivar Wennong 14

2014 ◽  
Vol 40 (5) ◽  
pp. 798 ◽  
Author(s):  
Wei SONG ◽  
Hui-Gai SUN ◽  
Yan-Ling SUN ◽  
Zi-Hui ZHAO ◽  
Xiao-Ming WANG ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Chromosomal Localization ◽  
Wheat Cultivar
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