scholarly journals Study of corneal epithelial progenitor origin and the Yap1 requirement using keratin 12 lineage tracing transgenic mice

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ramesh Babu Kasetti ◽  
Subhash Gaddipati ◽  
Shifu Tian ◽  
Lei Xue ◽  
Winston W.-Y. Kao ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Lineage Tracing ◽  
Corneal Epithelial ◽  
Keratin 12

MyoD-cre transgenic mice: A model for conditional mutagenesis and lineage tracing of skeletal muscle

genesis ◽  
2005 ◽  
Vol 41 (3) ◽  
pp. 116-121 ◽  
Author(s):  
Jennifer C. J. Chen ◽  
Justin Mortimer ◽  
Jason Marley ◽  
David J. Goldhamer
Keyword(s):  
Skeletal Muscle ◽  
Transgenic Mice ◽  
Lineage Tracing ◽  
Conditional Mutagenesis

scholarly journals Comparison of two related lines of tauGFP transgenic mice designed for lineage tracing

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Linda Sharp ◽  
Thomas Pratt ◽  
Gillian E. MacKay ◽  
Margaret A. Keighren ◽  
Jean H. Flockhart ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Lineage Tracing

scholarly journals Isolation and Chromosomal Localization of a Cornea-Specific Human Keratin 12 Gene and Detection of Four Mutations in Meesmann Corneal Epithelial Dystrophy

1997 ◽  
Vol 61 (6) ◽  
pp. 1268-1275 ◽  
Author(s):  
Kohji Nishida ◽  
Yoichi Honma ◽  
Atsuyoshi Dota ◽  
Satoshi Kawasaki ◽  
Wakako Adachi ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Corneal Epithelial ◽  
Keratin 12

scholarly journals Capturing limbal epithelial stem cell population dynamics, signature, and their niche

2020 ◽  
Author(s):  
Anna Altshuler ◽  
Aya Amitai-Lange ◽  
Noam Tarazi ◽  
Sunanda Dey ◽  
Lior Strinkovsky ◽  
...  
Keyword(s):  
Population Dynamics ◽  
Lineage Tracing ◽  
Stem Cell Population ◽  
Self Renewal ◽  
Neutral Drift ◽  
Corneal Epithelial ◽  
Depth Analysis ◽  
Dividing Cells ◽  
Slow Cycling ◽  
Stochastic Rules

AbstractStem cells (SCs) are traditionally viewed as rare, slow-cycling cells that follow deterministic rules dictating their self-renewal or differentiation. It was several decades ago, when limbal epithelial SCs (LSCs) that regenerate the corneal epithelium were one of the first sporadic, quiescent SCs ever discovered. However, LSC dynamics, heterogeneity and genetic signature are largely unknown. Moreover, recent accumulating evidence strongly suggested that epithelial SCs are actually abundant, frequently dividing cells that display stochastic behavior.In this work, we performed an in-depth analysis of the murine limbal epithelium by single-cell RNA sequencing and quantitative lineage tracing. The generated data provided an atlas of cell states of the corneal epithelial lineage, and particularly, revealed the co-existence of two novel LSC populations that reside in separate and well-defined sub-compartments. In the “outer” limbus, we identified a primitive widespread population of quiescent LSCs (qLSCs) that uniformly express Krt15/Gpha2/Ifitm3/Cd63 proteins, while the “inner” limbus host prevalent active LSCs (aLSCs) co-expressing Krt15-GFP/Atf3/Mt1-2/Socs3. Analysis of LSC population dynamics suggests that while qLSCs and aLSCs possess different proliferation rates, they both follow similar stochastic rules that dictate their self-renewal and differentiation. Finally, T cells were distributed in close proximity to qLSCs. Indeed, their absence or inhibition resulted in the loss of quiescence and delayed wound healing. Taken together, we propose that divergent regenerative strategies are tailored to properly support tissue-specific physiological constraints. The present study suggests that in the case of the cornea, quiescent epithelial SCs are abundant, follow stochastic rules and neutral drift dynamics.

Effect of Basic Fibroblast Growth Factor in Transgenic Mice: Corneal Epithelial Healing Process after Excimer Laser Photoablation

2008 ◽  
Vol 223 (2) ◽  
pp. 139-144 ◽  
Author(s):  
Alessandro Meduri ◽  
Sergio Zaccaria Scalinci ◽  
Mariachiara Morara ◽  
Piero Ceruti ◽  
Pier Luigi Grenga ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transgenic Mice ◽  
Fibroblast Growth Factor ◽  
Excimer Laser ◽  
Basic Fibroblast Growth Factor ◽  
Healing Process ◽  
Fibroblast Growth ◽  
Corneal Epithelial ◽  
Epithelial Healing

scholarly journals A Case of Solitary Nonvascularized Corneal Epithelial Dysplasia

2016 ◽  
Vol 2016 ◽  
pp. 1-4
Author(s):  
Tomoya Morii ◽  
Takayoshi Sumioka ◽  
Ai Izutani-Kitano ◽  
Yukihisa Takada ◽  
Yuka Okada ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Corneal Epithelium ◽  
Ocular Surface ◽  
Histopathological Examination ◽  
Precancerous Lesion ◽  
Intraepithelial Neoplasia ◽  
Epithelial Dysplasia ◽  
Corneal Epithelial ◽  
Keratin 13 ◽  
Keratin 12

Background. Epithelial dysplasia is categorized as conjunctival/corneal intraepithelial neoplasia which is a precancerous lesion. The lesion is usually developed at the limbal region and grows towards central cornea in association with neovascularization into the lesion. Here, we report a case of isolated nonvascularized corneal epithelial dysplasia surrounded by normal corneal epithelium with immune histochemical finding of ocular surface tissues cytokeratins, for example, keratin 13 and keratin 12.Case Presentation. A 76-year-old man consulted us for visual disturbance with localized opacification of the corneal epithelium in his left eye. His visual acuity was 20/20 and 20/200 in his right and left eye, respectively. Slit lamp examination showed a whitish plaque-like lesion at the center of his left corneal epithelium. No vascular invasion to the lesion was found. The lesion was surgically removed and subjected to histopathological examination and diagnosed as epithelial dysplasia. Amyloidosis was excluded by direct fast scarlet 4BS (DFS) staining. Immunohistochemistry showed that the dysplastic epithelial cells express keratin 13 and vimentin, but not keratin 12, indicating that the neoplastic epithelial cells lacked corneal-type epithelium differentiation.Conclusions. The lesion was diagnosed as nonvascularized epithelial dysplasia of ocular surface. Etiology of the lesion is not known.

scholarly journals Conditional Deletion of AP-2β in the Periocular Mesenchyme of Mice Alters Corneal Epithelial Cell Fate and Stratification

2021 ◽  
Vol 22 (16) ◽  
pp. 8730
Author(s):  
Haydn Walker ◽  
Aftab Taiyab ◽  
Paula Deschamps ◽  
Trevor Williams ◽  
Judith A. West-Mays
Keyword(s):  
Cell Fate ◽  
Corneal Endothelium ◽  
Anterior Segment ◽  
Corneal Epithelial Cell ◽  
Surface Ectoderm ◽  
Corneal Epithelial ◽  
Phenotypic Changes ◽  
Conditional Deletion ◽  
Bmp4 Expression ◽  
Keratin 12

The cornea is an anterior eye structure specialized for vision. The corneal endothelium and stroma are derived from the periocular mesenchyme (POM), which originates from neural crest cells (NCCs), while the stratified corneal epithelium develops from the surface ectoderm. Activating protein-2β (AP-2β) is highly expressed in the POM and important for anterior segment development. Using a mouse model in which AP-2β is conditionally deleted in the NCCs (AP-2β NCC KO), we investigated resulting corneal epithelial abnormalities. Through PAS and IHC staining, we observed structural and phenotypic changes to the epithelium associated with AP-2β deletion. In addition to failure of the mutant epithelium to stratify, we also observed that Keratin-12, a marker of the differentiated epithelium, was absent, and Keratin-15, a limbal and conjunctival marker, was expanded across the central epithelium. Transcription factors PAX6 and P63 were not observed to be differentially expressed between WT and mutant. However, growth factor BMP4 was suppressed in the mutant epithelium. Given the non-NCC origin of the epithelium, we hypothesize that the abnormalities in the AP-2β NCC KO mouse result from changes to regulatory signaling from the POM-derived stroma. Our findings suggest that stromal pathways such as Wnt/β-Catenin signaling may regulate BMP4 expression, which influences cell fate and stratification.

scholarly journals Mammalian face as an evolutionary novelty

2021 ◽  
Vol 118 (44) ◽  
pp. e2111876118
Author(s):  
Hiroki Higashiyama ◽  
Daisuke Koyabu ◽  
Tatsuya Hirasawa ◽  
Ingmar Werneburg ◽  
Shigeru Kuratani ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Lineage ◽  
Lineage Tracing ◽  
Evolutionary Novelty ◽  
Developmental Constraints ◽  
Mandibular Arch ◽  
Upper Jaw

The anterior end of the mammalian face is characteristically composed of a semimotile nose, not the upper jaw as in other tetrapods. Thus, the therian nose is covered ventrolaterally by the “premaxilla,” and the osteocranium possesses only a single nasal aperture because of the absence of medial bony elements. This stands in contrast to those in other tetrapods in whom the premaxilla covers the rostral terminus of the snout, providing a key to understanding the evolution of the mammalian face. Here, we show that the premaxilla in therian mammals (placentals and marsupials) is not entirely homologous to those in other amniotes; the therian premaxilla is a composite of the septomaxilla and the palatine remnant of the premaxilla of nontherian amniotes (including monotremes). By comparing topographical relationships of craniofacial primordia and nerve supplies in various tetrapod embryos, we found that the therian premaxilla is predominantly of the maxillary prominence origin and associated with mandibular arch. The rostral-most part of the upper jaw in nonmammalian tetrapods corresponds to the motile nose in therian mammals. During development, experimental inhibition of primordial growth demonstrated that the entire mammalian upper jaw mostly originates from the maxillary prominence, unlike other amniotes. Consistently, cell lineage tracing in transgenic mice revealed a mammalian-specific rostral growth of the maxillary prominence. We conclude that the mammalian-specific face, the muzzle, is an evolutionary novelty obtained by overriding ancestral developmental constraints to establish a novel topographical framework in craniofacial mesenchyme.

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