Prolongation of Larval-Pupal Development in Drosophila melanogaster and Its Effect on Facet Number

1945 ◽  
Vol 79 (782) ◽  
pp. 259-270
Author(s):  
Charles Ralph Williams
Keyword(s):  
Drosophila Melanogaster ◽  
Pupal Development ◽  
Facet Number

Relations between ecdysteroid levels and pupal development in the ecd-1 temperature-sensitive mutant of Drosophila melanogaster

1983 ◽  
Vol 29 (6) ◽  
pp. 509-514 ◽  
Author(s):  
S. Belinski-Deutsch ◽  
D. Busson ◽  
C. Lamour-Audit ◽  
P. Porcheron ◽  
M. Moriniere ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Pupal Development ◽  
Temperature Sensitive Mutant

Disruption of selenoprotein biosynthesis affects cell proliferation in the imaginal discs and brain of Drosophila melanogaster

1999 ◽  
Vol 112 (17) ◽  
pp. 2875-2884
Author(s):  
B. Alsina ◽  
M. Corominas ◽  
M.J. Berry ◽  
J. Baguna ◽  
F. Serras
Keyword(s):  
Cell Proliferation ◽  
Drosophila Melanogaster ◽  
Mrna Expression ◽  
Redox Balance ◽  
Imaginal Discs ◽  
Brdu Incorporation ◽  
Pupal Development ◽  
Localization Analysis ◽  
Dividing Cells

The patufet gene encodes the Drosophila melanogaster homologue of selenophosphate synthetase, an enzyme required for selenoprotein synthesis, and appears to have a role in cell proliferation. In this paper we analyse the expression pattern of patufet during the development of imaginal discs and brain as well as the function of this gene in relation to cell proliferation. Wild-type organisms showed a highly dynamic pattern of ptuf mRNA expression during larval and pupal development. Co-localization analysis of ptuf mRNA expression and BrdU incorporation showed high levels of ptuf mRNA in dividing cells and low or undetectable levels in non-dividing cells. In addition, [(75)Se] incorporation revealed a major selenoprotein band of 42 kDa. Mutant organisms showed no selenoprotein synthesis, lower levels of cell proliferation, a higher proportion of cells arrested in G(2) as seen by cyclin B labeling and increased levels of reactive oxygen species (ROS). Because most selenoproteins identified so far are antioxidants, the role of ptuf in cell proliferation through the control of the cellular redox balance is discussed.

scholarly journals forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster.

Genetics ◽  
1994 ◽  
Vol 136 (1) ◽  
pp. 173-182 ◽  
Author(s):  
N S Petersen ◽  
D H Lankenau ◽  
H K Mitchell ◽  
P Young ◽  
V G Corces
Keyword(s):  
Drosophila Melanogaster ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein S ◽  
Fiber Bundles ◽  
Alternative Promoters ◽  
Wild Type ◽  
Pupal Development ◽  
Coding Regions ◽  
Cytoplasmic Structures

Abstract The forked (f) gene of Drosophila melanogaster encodes six different transcripts 6.4, 5.6, 5.4, 2.5, 1.9, and 1.1 kb long. These transcripts arise by the use of alternative promoters. A polyclonal antibody raised against a domain common to all of the forked-encoded products has been used to identify forked proteins on two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and in Drosophila pupal tissues. The antibody stains fiber bundles present in bristle cells for about 15 hr during normal pupal development. Electron microscopy shows that these fibers are present from 40 to 53 hr in bristles of wild-type flies but are absent in the null f36a mutant. The forked protein(s) thus appear to be an essential part of the bristle fibers. The phenotype of the f36a mutation can be rescued by a 13-kb fragment of the forked locus containing the coding regions for the 2.5, 1.9, and 1.1-kb transcripts, suggesting that the proteins encoded by the three large forked RNAs are dispensable during bristle development. Increasing the copy number of a P[w+,f+] construct containing the 13-kb fragment induces a hypermorphic bristle phenotype whose severity correlates with the number of copies of P[w+,f+] present. These results indicate that alterations in the ratios among the forked proteins, or between forked products and other components of the fiber, result in abnormal assembly of the fibrillar cytoplasmic structures necessary for bristle morphogenesis.

scholarly journals Development of the indirect flight muscles of Drosophila

Development ◽  
1991 ◽  
Vol 113 (1) ◽  
pp. 67-77 ◽  
Author(s):  
J. Fernandes ◽  
M. Bate ◽  
K. Vijayraghavan
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
The Other ◽  
Specific Gene ◽  
Pupal Development ◽  
Specific Gene Expression ◽  
Morphological Criteria ◽  
Flight Muscles ◽  
Thoracic Muscles ◽  
Longitudinal Muscles

We have followed the pupal development of the indirect flight muscles (IFMs) of Drosophila melanogaster. At the onset of metamorphosis larval muscles start to histolyze, with the exception of a specific set of thoracic muscles. Myoblasts surround these persisting larval muscles and begin the formation of one group of adult indirect flight muscles, the dorsal longitudinal muscles. We show that the other group of indirect flight muscles, the dorsoventral muscles, develops simultaneously but without the use of larval templates. By morphological criteria and by patterns of specific gene expression, our experiments define events in IFM development.

Engrailed expression in the anterior lineage compartment of the developing wing blade of Drosophila

Development ◽  
1992 ◽  
Vol 115 (1) ◽  
pp. 21-33 ◽  
Author(s):  
S.S. Blair
Keyword(s):  
Gene Expression ◽  
Drosophila Melanogaster ◽  
Polyclonal Antiserum ◽  
Small Distance ◽  
Pupal Development ◽  
Cubitus Interruptus ◽  
Compartment Boundary ◽  
Enhancer Trap Line ◽  
Trap Line ◽  
Anterior Posterior

The developing wing of Drosophila melanogaster was examined at larval and pupal stages of development to determine whether the anterior-posterior lineage boundary, as identified by lineage restrictions, was congruent with the boundaries defined by the expression of posterior-specific (engrailed, invected), and anterior-specific (cubitus interruptus-D) genes. The lineage boundary was identified by marking mitotic recombinant clones, using an enhancer trap line with ubiquitous beta-gal expression in imaginal tissues; clones of +/+ cells were identified by their lack of beta-gal expression. Domains of gene expression were localized using antibodies and gene specific lacZ constructs. Surprisingly, it was found that engrailed expression extended a small distance into the anterior lineage compartment of the wing blade, as identified with anti-en/inv mAb, anti-en polyclonal antiserum, or an en-promoter-lacZ insert, ryxho25. This anterior expression was not present in early third instar discs, but appeared during subsequent larval and pupal development. In contrast, the expression of cubitus interruptus-D, as identified using the ci-Dplac insert, appeared to be limited to the anterior lineage compartment. Thus, en expression is not limited to cells from the posterior lineage compartment, and en and ci-D activities can overlap in a region just anterior to the lineage compartment boundary in the developing wing. The lineage boundary could also be identified by a line of aligned cells in the prospective wing blade region of wandering third instar discs. A decapentaplegic-lacZ construct was expressed in a stripe several cells anterior to the lineage boundary, and did not define or overlap into the posterior lineage compartment.

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