scholarly journals QUANTITATIVE CHEMICAL STUDIES ON HEMOLYSINS

1942 ◽  
Vol 25 (4) ◽  
pp. 523-531 ◽  
Author(s):  
Michael Heidelberger ◽  
Henry P. Treffers
Keyword(s):  
Guinea Pig ◽  
Chemical Studies ◽  
Quantitative Chemical

1. Total antibody in hemolysins may be estimated from the nitrogen added to sheep stromata suspensions. 2. The method is applied to a number of hemolysins and a correlation, valid to within 20 per cent, established between hemolytic titer and total antibody. 3. When stromata combine with antibody in the presence of guinea pig complement they may take up at least 80 per cent of their weight of complement combining component(s).

scholarly journals QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN

1942 ◽  
Vol 75 (3) ◽  
pp. 285-295 ◽  
Author(s):  
Michael Heidelberger ◽  
Manfred Mayer
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Human Complement ◽  
Modified Method ◽  
Chemical Studies ◽  
Quantitative Chemical ◽  
Pig Serum

1. A modified method is given for the titration of human complement so that C'1 titers are measured, as in guinea pig serum, instead of the C'2 titers yielded by the usual titration. 2. The measurement of complement combining component or components in weight units, instead of relative terms, is carried out as in guinea pig serum and leads to similar values, 0.03 to 0.05 mg. of C' N per ml. of human serum. 3. Other similarities in human and guinea pig complements are noted and discussed.

scholarly journals QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN

1941 ◽  
Vol 74 (4) ◽  
pp. 359-367 ◽  
Author(s):  
Michael Heidelberger ◽  
M. Rocha e Silva ◽  
Manfred Mayer
Keyword(s):  
Guinea Pig ◽  
Quantitative Data ◽  
Chemical Studies ◽  
Component C ◽  
Quantitative Chemical ◽  
Pig Serum

1. Quantitative data are given on the effect of variations in the time of contact and the proportions of the reactants on the quantity of complement combining component nitrogen (C'1 N) found in active guinea pig serum. 2. C'1 N was the same when determined with precipitates containing excess antibody or excess antigen. 3. Finely divided specific precipitates took up the complement combining component (C'1) from subsequently added guinea pig serum almost as well as specific precipitates formed in the presence of complement.

scholarly journals QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN

1941 ◽  
Vol 73 (6) ◽  
pp. 681-694 ◽  
Author(s):  
Michael Heidelberger
Keyword(s):  
Analytical Chemistry ◽  
New Method ◽  
Variable Volume ◽  
Chemical Studies ◽  
Quantitative Chemical

1. A quantitative micro method, conforming to the criteria of analytical chemistry, is proposed for the estimation of complement, or its combining component or components, in milligrams per milliliter instead of in the customary relative and variable volume units. 2. Data are given showing the range of accuracy and reproducibility of the new method.

scholarly journals Quantitative chemical studies in spinal fluids

1916 ◽  
Vol 14 (2) ◽  
pp. 26-27 ◽  
Author(s):  
R. L. Kahn ◽  
J. B. Neal
Keyword(s):  
Chemical Studies ◽  
Quantitative Chemical

scholarly journals PHENYLPYRUVIC OLIGOPHRENIA. REPORT OF A CASE IN AN INFANT WITH QUANTITATIVE CHEMICAL STUDIES OF THE URINE

1943 ◽  
Vol 22 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Margaret Dann ◽  
Eleanor Marples ◽  
Samuel Z. Levine
Keyword(s):  
Chemical Studies ◽  
Quantitative Chemical

scholarly journals QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN

1941 ◽  
Vol 73 (6) ◽  
pp. 695-709 ◽  
Author(s):  
Michael Heidelberger ◽  
Alfred J. Weil ◽  
Henry P. Treffers
Keyword(s):  
Methods Of Analysis ◽  
Chemical Studies ◽  
Antigen Antibody ◽  
Quantitative Chemical

1. The molecular quantities of hemolysin and complement combining component or components (C'1) involved in hemolysis have been calculated on the basis of new, quantitative, absolute methods of analysis. 2. Molecular combining ratios between antigen, antibody, and C'1 have been established. 3. The data are shown to be in accord with the theory of combination of multivalent antigen with multivalent antibody. 4. The fixation of complement by antigen-antibody combination is qualitatively and quantitatively accounted for on this basis.

scholarly journals VALUE AND LIMITATIONS OF QUANTITATIVE CHEMICAL STUDIES IN INDIVIDUAL CELLS

1969 ◽  
Vol 17 (3) ◽  
pp. 139-155 ◽  
Author(s):  
EZIO GIACOBINI
Keyword(s):  
High Precision ◽  
Nerve Cells ◽  
Chemical Studies ◽  
Quantitative Chemical

Remarkable methodologic advances in recent years make it possible to study with high precision, sensitivity and accuracy the activities of most enzymes and the levels of most substrates and metabolites in individual cells. The limits and the value of quantitative chemical studies in individual nerve cells are critically evaluated and discussed.

Structural Studies on Mitotic Spindle Fibres

1978 ◽  
Vol 36 (3) ◽  
pp. 615-626
Author(s):  
J.R. Mcintosh
Keyword(s):  
Chemical Composition ◽  
Mitotic Spindle ◽  
Structural Studies ◽  
Mitotic Apparatus ◽  
Chemical Studies ◽  
Medical Interest ◽  
Spindle Fibres

The mitotic apparatus is a structure of obvious biological and medical interest, but it has proved to be a difficult cellular machine to understand. The chemical composition of the spindle is only slightly elucidated, largely because of the difficulties in preparing useful isolates of the structure. Chemical studies of the mitotic spindle have been reviewed elsewhere (Mcintosh, 1977), and will not be discussed further here. One would think that structural studies on the mitotic apparatus (MA) in situ would be straightforward, but even with this approach there is some disagreement in the results obtained with various methods and by different investigators. In this paper I will review briefly the approaches which have been used in structural studies of the MA, pointing out the strengths and problems of each approach. I will summarize the principal findings of the different methods, and identify what seem to be fruitful avenues for further work.

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