scholarly journals QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN

1941 ◽  
Vol 74 (4) ◽  
pp. 359-367 ◽  
Author(s):  
Michael Heidelberger ◽  
M. Rocha e Silva ◽  
Manfred Mayer
Keyword(s):  
Guinea Pig ◽  
Quantitative Data ◽  
Chemical Studies ◽  
Component C ◽  
Quantitative Chemical ◽  
Pig Serum

1. Quantitative data are given on the effect of variations in the time of contact and the proportions of the reactants on the quantity of complement combining component nitrogen (C'1 N) found in active guinea pig serum. 2. C'1 N was the same when determined with precipitates containing excess antibody or excess antigen. 3. Finely divided specific precipitates took up the complement combining component (C'1) from subsequently added guinea pig serum almost as well as specific precipitates formed in the presence of complement.

scholarly journals QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN

1942 ◽  
Vol 75 (3) ◽  
pp. 285-295 ◽  
Author(s):  
Michael Heidelberger ◽  
Manfred Mayer
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Human Complement ◽  
Modified Method ◽  
Chemical Studies ◽  
Quantitative Chemical ◽  
Pig Serum

1. A modified method is given for the titration of human complement so that C'1 titers are measured, as in guinea pig serum, instead of the C'2 titers yielded by the usual titration. 2. The measurement of complement combining component or components in weight units, instead of relative terms, is carried out as in guinea pig serum and leads to similar values, 0.03 to 0.05 mg. of C' N per ml. of human serum. 3. Other similarities in human and guinea pig complements are noted and discussed.

scholarly journals QUANTITATIVE CHEMICAL STUDIES ON HEMOLYSINS

1942 ◽  
Vol 25 (4) ◽  
pp. 523-531 ◽  
Author(s):  
Michael Heidelberger ◽  
Henry P. Treffers
Keyword(s):  
Guinea Pig ◽  
Chemical Studies ◽  
Quantitative Chemical

1. Total antibody in hemolysins may be estimated from the nitrogen added to sheep stromata suspensions. 2. The method is applied to a number of hemolysins and a correlation, valid to within 20 per cent, established between hemolytic titer and total antibody. 3. When stromata combine with antibody in the presence of guinea pig complement they may take up at least 80 per cent of their weight of complement combining component(s).

Effect of Bentonite on Fibrinolytic System in Serum

1963 ◽  
Vol 10 (01) ◽  
pp. 120-132 ◽  
Author(s):  
E. S Olesen
Keyword(s):  
Guinea Pig ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
High Potential ◽  
Fibrinolytic System ◽  
Fibrinolytic Agents ◽  
Isoelectric Precipitation ◽  
Active Protease ◽  
Pig Serum

SummaryTreatment of serum with bentonite led to a reduced content of inhibitors of trypsin and urokinase in the isoelectrically precipitated euglobulin, and removed fibrinolytic agents and precursors from serum. Bentonite-treated serum added to untreated serum reduced precipitation of the above inhibitors, and presumably also precipitation of inhibitors against a plasminogen activator of serum.Bentonite-treated serum (whether from pig, ox, guinea-pig, or man), added to untreated guinea-pig serum, produced fibrinolytic activity on isoelectric precipitation of the mixture; the activity of the euglobulin was due to an activator of plasminogen as well as an active protease, probably plasmin. The described effects of bentonite-treated serum are similar to those previously reported for anionic polyelectrolytes. Possible mechanisms are discussed.The “non-specific” activation of fibrinolytic activity by means of bentonite emphasizes that guinea-pig serum [which is characterized by a high potential for “nonspecific” activation of its fibrinolytic system Olesen (1962)] contains all the elements required for the formation of an activator of plasminogen, and thus the activation of its plasminogen to plasmin.

scholarly journals Interactions of C-reactive protein with the complement system. III. Complement-dependent passive hemolysis initiated by CRP.

1975 ◽  
Vol 142 (5) ◽  
pp. 1065-1077 ◽  
Author(s):  
A.P. Osmand ◽  
R.F. Mortensen ◽  
Joan Siegel ◽  
H. Gewurz
Keyword(s):  
Guinea Pig ◽  
Human Serum ◽  
Complete System ◽  
Gel Filtration ◽  
C Reactive Protein ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Rabbit Igg ◽  
The Complement System ◽  
Pig Serum

Interactions of CRP with various substrates in the presence of human serum have been shown to result in efficient activation of C components C1-C5. We now report the ability of CRP to initiate C-dependent hemolysis. For this purpose CRP was isolated by affinity chromatography using pneumococcal CPS and gel filtration; its purity was established by several criteria. Erythrocytes were coated with CPS (E-CPS) and passively sensitized with CRP. C-dependent lysis of these cells was observed upon the addition of suitably absorbed human serum, and the efficiency of hemolysis compared favorably with that initiated by rabbit IgG anti-CPS antibody. CRP also sensitized E-CPS for lysis by guinea pig C; partial lysis was seen when C4-deficient guinea pig serum was used, suggesting that CRP also shares with antibody the ability of CRP to fully activate the C system and provide further evidence for a role for CRP similar to that of antibody in the initiation and modulation of inflammatory reactions via the complete system.

Electrophoretic Studies of Normal Rovine and Guinea-Pig Serum

1963 ◽  
Vol 4 (1) ◽  
pp. 64-84
Author(s):  
I. Koefoed Jensen
Keyword(s):  
Guinea Pig ◽  
Pig Serum

scholarly journals Guinea Pig Serum and Liver l-Asparaginases

1970 ◽  
Vol 245 (11) ◽  
pp. 2797-2801 ◽  
Author(s):  
Helga M. Suld ◽  
Peter A. Herbut
Keyword(s):  
Guinea Pig ◽  
Pig Serum

scholarly journals EVIDENCE THAT THE L-ASPARAGINASE OF GUINEA PIG SERUM IS RESPONSIBLE FOR ITS ANTILYMPHOMA EFFECTS

1963 ◽  
Vol 118 (1) ◽  
pp. 99-120 ◽  
Author(s):  
J. D. Broome
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Serum Proteins ◽  
Deae Cellulose ◽  
Salt Precipitation ◽  
Asparaginase Activity ◽  
Pig Serum

A number of the properties of the L-asparaginase present in guinea pig serum have been examined and shown to be indistinguishable from those of the agent responsible for inhibiting cells of lymphoma 6C3HED in vivo. The patterns of instability of the enzyme to changes in temperature and pH were found to parallel closely those of the antilymphoma agent. L-Asparaginase activity was essentially absent from the serum of newborn guinea pigs and this failed to inhibit 6C3HED cells. On separating guinea pig serum proteins by salt precipitation, electrophoresis, and chromatography on DEAE cellulose, antilymphoma activity was found only in fractions which contained L-asparaginase.

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