scholarly journals PERFORMANCE OF THE HEPP MICRO-OSMOMETER

1939 ◽  
Vol 23 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Erich Peters ◽  
George Saslow
Keyword(s):  
Molecular Weight ◽  
Serum Albumin ◽  
Osmotic Pressure ◽  
Horse Serum ◽  
Accuracy And Precision

Estimation of the molecular weight of horse serum albumin from the osmotic pressure of solutions containing 0.713 to 5.12 gm. per 100 cc. shows that the Hepp osmometer yields the same values as the standard simple osmometer of Adair. Accuracy and precision of the instrument decrease noticeably at concentrations of albumin less than 0.7 gm. per 100 cc. A determination in duplicate can be carried out with this instrument in less than 2 hours. The instrument is easily operated.

scholarly journals ABSENCE OF METHIONINE IN CRYSTALLINE HORSE SERUM ALBUMIN

1941 ◽  
Vol 141 (3) ◽  
pp. 999-1000
Author(s):  
Erwin Brand ◽  
Beatrice Kassell
Keyword(s):  
Serum Albumin ◽  
Horse Serum

scholarly journals Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3321
Author(s):  
Katarzyna Kurpet ◽  
Rafał Głowacki ◽  
Grażyna Chwatko
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Reversed Phase ◽  
Fluorescence Labeling ◽  
Detector Response ◽  
Low Molecular Weight

Biothiols are extremely powerful antioxidants that protect cells against the effects of oxidative stress. They are also considered relevant disease biomarkers, specifically risk factors for cardiovascular disease. In this paper, a new procedure for the simultaneous determination of human serum albumin and low-molecular-weight thiols in plasma is described. The method is based on the pre-column derivatization of analytes with a thiol-specific fluorescence labeling reagent, monobromobimane, followed by separation and quantification through reversed-phase high-performance liquid chromatography with fluorescence detection (excitation, 378 nm; emission, 492 nm). Prior to the derivatization step, the oxidized thiols are converted to their reduced forms by reductive cleavage with sodium borohydride. Linearity in the detector response for total thiols was observed in the following ranges: 1.76–30.0 mg mL−1 for human serum albumin, 0.29–5.0 nmol mL−1 for α-lipoic acid, 1.16–35 nmol mL−1 for glutathione, 9.83–450.0 nmol mL−1 for cysteine, 0.55–40.0 nmol mL−1 for homocysteine, 0.34–50.0 nmol mL−1 for N-acetyl-L-cysteine, and 1.45–45.0 nmol mL−1 for cysteinylglycine. Recovery values of 85.16–119.48% were recorded for all the analytes. The developed method is sensitive, repeatable, and linear within the expected ranges of total thiols. The devised procedure can be applied to plasma samples to monitor biochemical processes in various pathophysiological states.

scholarly journals NOTE ON CHANGES IN HORSE SERUM ALBUMIN ON AGING

1944 ◽  
Vol 156 (2) ◽  
pp. 777-778
Author(s):  
Dan H. Moore ◽  
Manfred Mayer
Keyword(s):  
Serum Albumin ◽  
Horse Serum

The Conjugation of Horse Serum Albumin with 1,2-Benzanthryl Isocyanates1

1940 ◽  
Vol 62 (8) ◽  
pp. 1970-1975 ◽  
Author(s):  
Hugh J. Creech ◽  
R. Norman Jones
Keyword(s):  
Serum Albumin ◽  
Horse Serum
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