The Conjugation of Horse Serum Albumin with 1,2-Benzanthryl Isocyanates1

1940 ◽  
Vol 62 (8) ◽  
pp. 1970-1975 ◽  
Author(s):  
Hugh J. Creech ◽  
R. Norman Jones
Keyword(s):  
Serum Albumin ◽  
Horse Serum

scholarly journals ABSENCE OF METHIONINE IN CRYSTALLINE HORSE SERUM ALBUMIN

1941 ◽  
Vol 141 (3) ◽  
pp. 999-1000
Author(s):  
Erwin Brand ◽  
Beatrice Kassell
Keyword(s):  
Serum Albumin ◽  
Horse Serum

scholarly journals NOTE ON CHANGES IN HORSE SERUM ALBUMIN ON AGING

1944 ◽  
Vol 156 (2) ◽  
pp. 777-778
Author(s):  
Dan H. Moore ◽  
Manfred Mayer
Keyword(s):  
Serum Albumin ◽  
Horse Serum

scholarly journals Some observations on the reaction between horse serum and a pool of rabbit antihorse serum. II. An analysis of the antigens concerned in the production of multiple zones

1949 ◽  
Vol 47 (4) ◽  
pp. 360-383
Author(s):  
G. R. E. Naylor
Keyword(s):  
Serum Albumin ◽  
Horse Serum ◽  
Electrophoretic Analysis ◽  
Precipitation Reaction ◽  
Serum Globulin ◽  
Antigenic Analysis ◽  
Single Antigen ◽  
Albumin Preparation ◽  
Series Of Experiments ◽  
Concordant Result

1. The reaction horse serum and a pool of rabbit antihorse serum exhibiting four α procedure optima has been investigated with a view to analysing the antigens involved in the production of the multiple zones.2. The following conclusions have been reached regarding the antigenic constitution of horse serum, a horse serum-albumin preparation and a horse serum-globulin preparation: Horse serum consists of at least five antigens. The horse serum-albumin preparation contains three antigens, two of which are probably impurities, one of these being a globulin. The horse serum-globulin preparation consists of three antigens and evidence was obtained of a fourth. Electrophoretic analysis of these protein preparations gave concordant result.3. Multiple zones of rapid particulation in the precipitation reaction are due to the independent activity of multiple antigens and their homologous antibodies.4. The absorption of precipitin from a mixture of antibodies by a mixture of antigens at a single α procedure optimum is both a useful and a practicable procedures.5. Attention is drawn to the fact that the method of antigenic analysis used in the present series of experiments, involving the correlation of α; procedure optima occurring in the parallel titrations of a single antiserum against a mixed antigen and equivalent concentrations of physically or chemically prepared fractions of it supplemented by absorption of precipitins at individual and multiple α precedure optima and retitration of the absorbed sera, could be applied to the identification and nomenclature of proteins occurring in natural mixtures, and also to the quantitative and qualitative appraisal of the purity of proteins prepared by physical or chemical means. The method does not rely on the preparation of highly purified protein samples containing a single antigen.

scholarly journals HISTOLOGICAL AND SEROLOGICAL SEQUENCES IN EXPERIMENTAL HYPERSENSITIVITY

1947 ◽  
Vol 85 (6) ◽  
pp. 571-590 ◽  
Author(s):  
Clinton Van Zandt Hawn ◽  
Charles A. Janeway
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gamma Globulin ◽  
Horse Serum ◽  
Protein Solutions ◽  
Pathological Lesions ◽  
Intravenous Injections ◽  
Foreign Proteins ◽  
Antigen Antibody

1. Groups of normal rabbits were given, single intravenous injections of foreign proteins in doses of 1 gm. per kilo, bled at regular intervals for serologic studies, and sacrificed after varying lengths of time for pathological studies. The protein solutions used were of crystallized bovine serum albumin, bovine serum gamma globulin, and bovine serum. The experiments were planned, first, to correlate the sequence of pathological and immunological changes, and second, to compare the responses to two chemically and immunologically distinct plasma protein fractions and to the whole serum of the same species. 2. (a) The principal pathological lesions in rabbits given bovine serum were similar to those which have been previously observed following, the injection of horse serum and were characterized by widely dispersed but segmental acute inflammatory lesions of the arteries. These lesions were at their height 2 weeks after injection and showed marked repair at 4 weeks. (b) Crystallized bovine serum albumin produced lesions almost exclusively confined to the arteries which were at their height at 2 weeks, were healing at 3, and healed by 4 weeks. The lesions were less numerous and less intense than in animals given whole serum and were only found in some of the animals. (c) Bovine serum gamma globulin elicited quite different histologic sequences. The most striking lesions involved the glomeruli of the kidneys, and to a lesser degree, the heart. Lesions in the liver and joints were present but less conspicuous, and arterial lesions were rare and slight in degree. The lesions not only differed from those in rabbits given albumin in distribution but in timing, since they were most widespread and acute at 1 week and were healing at 2 weeks after injection. Moreover, lesions were observed in almost every animal. 3. Results of immunological studies were consistent with the interpretation that the pathological lesions were due to an antigen-antibody reaction in the tissues, as shown by the following: (a) Acute lesions were only observed when antigen was present and before antibody appeared in the circulation. (b) Healing of lesions was only observed (with one exception) when antigen had almost or completely disappeared from the circulation, usually with the appearance of antibody. (c) There was a correlation between the rapidity of evolution of the lesions and the rapidity with which the antigen disappeared from the circulation. (d) There was a rough correlation between the proportion of animals showing lesions and the proportion developing antibodies after the injection of a particular protein solution.

scholarly journals THE LYMPHATIC PATHWAY FROM THE NOSE AND PHARYNX

1938 ◽  
Vol 68 (6) ◽  
pp. 941-947 ◽  
Author(s):  
Joseph M. Yoffey ◽  
Eugene R. Sullivan ◽  
Cecil K. Drinker
Keyword(s):  
Serum Albumin ◽  
Serum Proteins ◽  
Horse Serum ◽  
Protein Solutions ◽  
Cent Solution ◽  
Egg Albumin ◽  
Lymphatic Pathway

1. In a number of cats, dogs, monkeys, and in a rabbit, the cervical lymph ducts were cannulated and protein solutions dropped into the nose, and the lymph was examined afterwards for the presence of the protein employed. 2. Egg albumin was found in the lymph in all cases with one exception. Horse serum was never detected. Serum albumin did not come through in the cats, but did in a rabbit. 3. With a 1 per cent solution of T-1824 in horse serum no dye appeared in the lymph. This is regarded as confirming Gregersen and Gibson's (2) view that T-1824 combines with the serum proteins.

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