scholarly journals “Optical Patch-clamping”

2005 ◽  
Vol 126 (3) ◽  
pp. 179-192 ◽  
Author(s):  
Angelo Demuro ◽  
Ian Parker
Keyword(s):  
Kinetic Resolution ◽  
Spatial Information ◽  
Single Channel ◽  
Bathing Solution ◽  
Patch Clamp Recording ◽  
New Approach ◽  
Order Of Magnitude ◽  
Nicotinic Receptor Channels ◽  
Fluorescence Transients ◽  
Magnitude Improvement

We describe an optical technique using total internal reflection fluorescence (TIRF) microscopy to obtain simultaneous and independent recordings from numerous ion channels via imaging of single-channel Ca2+ flux. Muscle nicotinic acetylcholine (ACh) receptors made up of αβγδ subunits were expressed in Xenopus oocytes, and single channel Ca2+ fluorescence transients (SCCaFTs) were imaged using a fast (500 fps) electron-multiplied c.c.d. camera with fluo-4 as the indicator. Consistent with their arising through openings of individual nicotinic channels, SCCaFTs were seen only when a nicotinic agonist was present in the bathing solution, were blocked by curare, and increased in frequency as roughly the second power of [ACh]. Their fluorescence amplitudes varied linearly with membrane potential and extrapolated to zero at about +60 mV. The rise and fall times of fluorescence were as fast as 2 ms, providing a kinetic resolution adequate to characterize channel gating kinetics; which showed mean open times of 7.9 and 15.8 ms when activated, respectively, by ACh or suberyldicholine. Simultaneous records were obtained from >400 channels in the imaging field, and we devised a novel “channel chip” representation to depict the resultant large dataset as a single image. The positions of SCCaFTs remained fixed (<100 nm displacement) over tens of seconds, indicating that the nicotinic receptor/channels are anchored in the oocyte membrane; and the spatial distribution of channels appeared random without evidence of clustering. Our results extend single-channel TIRFM imaging to ligand-gated channels that display only partial permeability to Ca2+, and demonstrate an order-of-magnitude improvement in kinetic resolution. We believe that functional single-channel imaging opens a new approach to ion channel study, having particular advantages over patch-clamp recording in that it is massively parallel, and provides high-resolution spatial information that is inaccessible by electrophysiological techniques.

High-Density Memristor-CMOS Ternary Logic Family

2020 ◽  
Author(s):  
Xiaoyuan Wang ◽  
Pengfei Zhou ◽  
Jason Eshraghian ◽  
Chih-Yang Lin ◽  
Herbert Ho-Ching Iu ◽  
...  
Keyword(s):  
Indium Tin Oxide ◽  
Practical Implementation ◽  
Switching Speed ◽  
High Data ◽  
Fast Switching ◽  
Data Density ◽  
Order Of Magnitude ◽  
High Transconductance ◽  
Logic Functions ◽  
Magnitude Improvement

<div>This paper presents the first experimental demonstration</div><div>of a ternary memristor-CMOS logic family. We systematically</div><div>design, simulate and experimentally verify the primitive</div><div>logic functions: the ternary AND, OR and NOT gates. These are then used to build combinational ternary NAND, NOR, XOR and XNOR gates, as well as data handling ternary MAX and MIN gates. Our simulations are performed using a 50-nm process which are verified with in-house fabricated indium-tin-oxide memristors, optimized for fast switching, high transconductance, and low current leakage. We obtain close to an order of magnitude improvement in data density over conventional CMOS logic, and a reduction of switching speed by a factor of 13 over prior state-of-the-art ternary memristor results. We anticipate extensions of this work can realize practical implementation where high data density is of critical importance.</div>

scholarly journals Incremental Construction of Generalized Voronoi Diagrams on Pointerless Quadtrees

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Quanjun Yin ◽  
Long Qin ◽  
Xiaocheng Liu ◽  
Yabing Zha
Keyword(s):  
Voronoi Diagrams ◽  
Surrounding Area ◽  
Repair Mechanism ◽  
Local Repair ◽  
Order Of Magnitude ◽  
Generalized Voronoi Diagrams ◽  
Incremental Construction ◽  
Coarse To Fine ◽  
Multiple Granularities ◽  
Magnitude Improvement

In robotics, Generalized Voronoi Diagrams (GVDs) are widely used by mobile robots to represent the spatial topologies of their surrounding area. In this paper we consider the problem of constructing GVDs on discrete environments. Several algorithms that solve this problem exist in the literature, notably the Brushfire algorithm and its improved versions which possess local repair mechanism. However, when the area to be processed is very large or is of high resolution, the size of the metric matrices used by these algorithms to compute GVDs can be prohibitive. To address this issue, we propose an improvement on the current algorithms, using pointerless quadtrees in place of metric matrices to compute and maintain GVDs. Beyond the construction and reconstruction of a GVD, our algorithm further provides a method to approximate roadmaps in multiple granularities from the quadtree based GVD. Simulation tests in representative scenarios demonstrate that, compared with the current algorithms, our algorithm generally makes an order of magnitude improvement regarding memory cost when the area is larger than210×210. We also demonstrate the usefulness of the approximated roadmaps for coarse-to-fine pathfinding tasks.

Half division two-dimensional method for simple sinusoidal quantities parameters accurate measurement

2021 ◽  
pp. 16-21
Author(s):  
Kirill Yu. Solomentsev ◽  
Vyacheslav I. Lachin ◽  
Aleksandr E. Pasenchuk
Keyword(s):  
Measuring Instruments ◽  
Measured Signal ◽  
Two Dimensional ◽  
Measuring Device ◽  
New Approach ◽  
Precision Measuring ◽  
Useful Signal ◽  
Midpoint Method ◽  
Order Of Magnitude ◽  
Current Measuring

Several variants of half division two-dimensional method are proposed, which is the basis of a fundamentally new approach for constructing measuring instruments for sinusoidal or periodic electrical quantities. These measuring instruments are used in the diagnosis of electric power facilities. The most general variant, called midpoint method, is considered. The proposed midpoint method allows you to measure much smaller than using widespread methods, alternating currents or voltages, especially when changing the amplitude of the measured signal in very wide ranges, by 1–2 orders of magnitude. It is shown that using the midpoint method it is possible to suppress sinusoidal or periodic interference in the measuring path, in particular, to measure small alternating current when sinusoidal or periodic interference is 1–2 orders of magnitude higher than the useful signal. Based on the results of comparative tests, it was found that the current measuring device implementing the midpoint method is an order of magnitude more sensitive than the currently used high-precision measuring instruments.

Enzymatic Dynamic Kinetic Resolution of Oxazinones: A New Approach to Enantiopure β2-Amino Acids

ChemCatChem ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 319-330 ◽  
Author(s):  
Albrecht Berkessel ◽  
Ilona Jurkiewicz ◽  
Resmi Mohan
Keyword(s):  
Amino Acids ◽  
Kinetic Resolution ◽  
Dynamic Kinetic Resolution ◽  
New Approach

scholarly journals The Array of Long Baseline Antennas for Taking Radio Observations from the Sub-Antarctic

2020 ◽  
Vol 09 (04) ◽  
pp. 2050019
Author(s):  
H. C. Chiang ◽  
T. Dyson ◽  
E. Egan ◽  
S. Eyono ◽  
N. Ghazi ◽  
...  
Keyword(s):  
Low Frequency ◽  
Neutral Hydrogen ◽  
Radio Frequency Interference ◽  
Marion Island ◽  
Radio Observations ◽  
Long Baseline ◽  
Galactic Emission ◽  
Operating Frequency Range ◽  
Order Of Magnitude ◽  
Magnitude Improvement

Measurements of redshifted 21[Formula: see text]cm emission of neutral hydrogen at [Formula: see text][Formula: see text]MHz have the potential to probe the cosmic “dark ages,” a period of the universe’s history that remains unobserved to date. Observations at these frequencies are exceptionally challenging because of bright Galactic foregrounds, ionospheric contamination, and terrestrial radio-frequency interference. Very few sky maps exist at [Formula: see text][Formula: see text]MHz, and most have modest resolution. We introduce the Array of Long Baseline Antennas for Taking Radio Observations from the Sub-Antarctic (ALBATROS), a new experiment that aims to image low-frequency Galactic emission with an order-of-magnitude improvement in resolution over existing data. The ALBATROS array will consist of antenna stations that operate autonomously, each recording baseband data that will be interferometrically combined offline. The array will be installed on Marion Island and will ultimately comprise 10 stations, with an operating frequency range of 1.2–125[Formula: see text]MHz and maximum baseline lengths of [Formula: see text][Formula: see text]km. We present the ALBATROS instrument design and discuss pathfinder observations that were taken from Marion Island during 2018–2019.

scholarly journals The Pore Architecture of the Cystic Fibrosis Transmembrane Conductance Regulator Channel Revealed by Co-Mutation in Pore-Forming Transmembrane Regions

2016 ◽  
pp. 505-515
Author(s):  
F. QIAN ◽  
L. LIU ◽  
Z. LIU ◽  
C. LU
Keyword(s):  
Cystic Fibrosis ◽  
Single Channel ◽  
Transmembrane Conductance Regulator ◽  
Patch Clamp Recording ◽  
Transmembrane Conductance ◽  
Channel Pore ◽  
Selective Filter ◽  
Transmembrane Regions ◽  
Cystic Fibrosis Transmembrane ◽  
Insight Into

The cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel contains 12 transmembrane (TM) regions that are presumed to form the channel pore. However, there is no direct evidence clearly illustrating the involvement of these transmembrane regions in the actual CFTR pore structure. To obtain insight into the architecture of the CFTR channel pore, we used patch clamp recording techniques and a strategy of co-mutagenesis of two potential pore-forming transmembrane regions (TM1 and TM6) to investigate the collaboration of these two TM regions. We performed a range of specific functional assays comparing the single channel conductance, anion binding, and anion selectivity properties of the co-mutated CFTR variants, and the results indicated that TM1 and TM6 play vital roles in forming the channel pore and, thus, determine the functional properties of the channel. Furthermore, we provided functional evidence that the amino acid threonine (T338) in TM6 has synergic effects with lysine (K95) in TM1. Therefore, we propose that these two residues have functional collaboration in the CFTR channel pore and may collectively form a selective filter.

scholarly journals Low error discrimination using a correlated population code

2012 ◽  
Vol 108 (4) ◽  
pp. 1069-1088 ◽  
Author(s):  
Greg Schwartz ◽  
Jakob Macke ◽  
Dario Amodei ◽  
Hanlin Tang ◽  
Michael J. Berry
Keyword(s):  
Spatial Information ◽  
Ganglion Cells ◽  
Discrimination Performance ◽  
Spike Timing ◽  
Multiple Time ◽  
Entropy Model ◽  
Decoding Algorithms ◽  
Single Spike ◽  
Order Of Magnitude ◽  
Spike Latency

We explored the manner in which spatial information is encoded by retinal ganglion cell populations. We flashed a set of 36 shape stimuli onto the tiger salamander retina and used different decoding algorithms to read out information from a population of 162 ganglion cells. We compared the discrimination performance of linear decoders, which ignore correlation induced by common stimulation, with nonlinear decoders, which can accurately model these correlations. Similar to previous studies, decoders that ignored correlation suffered only a modest drop in discrimination performance for groups of up to ∼30 cells. However, for more realistic groups of 100+ cells, we found order-of-magnitude differences in the error rate. We also compared decoders that used only the presence of a single spike from each cell with more complex decoders that included information from multiple spike counts and multiple time bins. More complex decoders substantially outperformed simpler decoders, showing the importance of spike timing information. Particularly effective was the first spike latency representation, which allowed zero discrimination errors for the majority of shape stimuli. Furthermore, the performance of nonlinear decoders showed even greater enhancement compared with linear decoders for these complex representations. Finally, decoders that approximated the correlation structure in the population by matching all pairwise correlations with a maximum entropy model fit to all 162 neurons were quite successful, especially for the spike latency representation. Together, these results suggest a picture in which linear decoders allow a coarse categorization of shape stimuli, whereas nonlinear decoders, which take advantage of both correlation and spike timing, are needed to achieve high-fidelity discrimination.

Spatial Signature Algorithm (SSA): A New Approach in Countermeasuring XML Signature Wrapping Attack

2019 ◽  
Vol 892 ◽  
pp. 249-257
Author(s):  
Madihah Mohd Saudi ◽  
Nurzi Juana Mohd Zaizi ◽  
Azreena Abu Bakar ◽  
Khaled Juma Ahmed Swessi
Keyword(s):  
Digital Signature ◽  
Spatial Information ◽  
Simple Object ◽  
Simple Object Access Protocol ◽  
Access Protocol ◽  
New Approach ◽  
Computational Expense ◽  
Specific Disease ◽  
Xml Signature

This paper introduces a new approach in countermeasuring XML signature wrapping attack called the Spatial Signature Algorithm (SSA). The motivation for proposing the SSA approach is due to the limitation of the SOAP (Simple Object Access Protocol) in handling the XML signature wrapping attacks. A different strategy is to be planned in order to deter such attack without extensive computational expense. Spatial Signature Algorithm builds upon the notion of ratio signature that is recommended by a research in biotechnology. The research suggests the possibility of diagnosing a specific disease based on the idea of ratios, specifically on the comparative relationship between elements to detect the emergence of certain threats. Bridging this notion to security, the principle of using space and ratio to detect abnormality is extended to the application of spatial information and digital signature to detect and combat the XML wrapping signature attack.

Voltage-gated currents of rabbit A- and B-type horizontal cells in retinal monolayer cultures

1994 ◽  
Vol 11 (2) ◽  
pp. 369-378 ◽  
Author(s):  
Stefan Löhrke ◽  
Hans-Dieter Hofmann
Keyword(s):  
Single Channel ◽  
Ionic Currents ◽  
Calcium Currents ◽  
Horizontal Cells ◽  
Delayed Rectifier ◽  
Patch Clamp Recording ◽  
Voltage Gated ◽  
Monolayer Cultures ◽  
Inward Currents

AbstractIn monolayer cultures prepared from immature early postnatal rabbit retina, small populations of neurons can be demonstrated to differentiate into apparently mature A- and B-type horizontal cells. Using wholecell, single-channel, patch-clamp recording techniques, we have analyzed the pattern of voltage-gated conductances expressed by mammalian horizontal cells under these conditions. A total of six different voltage-dependent ionic currents were recorded. Tetrodotoxin-sensitive fast sodium inward currents (INa) were found in 81% of the A-type and 90% of the B-type cells. Inward calcium currents could be demonstrated in all cells tested after blockade of other conductances. Two types of outward potassium currents with properties of the 4–aminopyridine-sensitive transient IA and the tetraethylammonium sensitive delayed rectifier IK, respectively, could be characterized in whole-cell recordings. An inward rectifying potassium current (Ianom) typical for horizontal cells was activated in response to hyperpolarizing voltage steps. These types of currents have also been described in dissociated adult horizontal cells from lower vertebrates and cat. With single-channel recordings on inside-out patches excised from B-type cells, an additional Ca2+-dependent current (IK(Ca)) was observed which, so far, has not been described in horizontal cells developing in situ. Our results demonstrate that cultured rabbit horizontal cells express a set of voltage-gated currents which largely, but not completely, corresponds to that described in situ for horizontal cells of other species. The culture system will allow further investigation of developmental and functional aspects of mammalian horizontal cells.

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