scholarly journals A PHYSICAL-CHEMICAL DIFFERENCE IN ANTIBODIES AGAINST THE S AND R VARIANTS OF A SINGLE BACTERIAL STRAIN

1935 ◽  
Vol 18 (5) ◽  
pp. 615-625 ◽  
Author(s):  
Eleanore W. Joffe

1. Rabbits were immunized with Bact. typhosum 0 901 S and 0 901 R, over a long period. Homologous and heterologous strains were sensitized with sera obtained from weekly bleedings. Agglutination titer was recorded, and the isoelectric points of the bacteria maximally sensitized were determined. 2. 0 901 S maximally sensitized with homologous immune serum had isoelectric points which became more alkaline as immunization progressed, covering a range of pH 4.8 to 5.5. 3. Strain 0 901 R maximally sensitized with homologous immune serum had isoelectric points which became more alkaline as immunization progressed, covering the range of pH 5.0 to 5.9. 4. Both 0 901 S and 0 901 R maximally sensitized with heterologous serum had isoelectric points lower than when sensitized with homologous serum. 5. The isoelectric points of both forms sensitized with increasing concentrations of homologous immune serum were determined. Increasing concentrations of homologous immune serum shifted the isoelectric point of 0 901 R from less than 2.2 for the unsensitized bacteria progressively to the alkaline side until the maximum values previously mentioned were reached. Increasing concentrations of homologous immune serum conferred upon 0 901 S isoelectric points which became only slightly more alkaline in maximal sensitization. 6. The electrophoretic mobilities of 0 901 S and 0 901 R, in each case maximally sensitized with homologous hyperimmune serum, were found to differ significantly over the whole range of pH studied.

1930 ◽  
Vol 13 (6) ◽  
pp. 669-681 ◽  
Author(s):  
Morton McCutcheon ◽  
Stuart Mudd ◽  
Max Strumia ◽  
Balduin Lucké

Sensitization with increasing concentrations of homologous immune serum shifts the isoelectric point of the antigens studied progressively to the alkaline side. Antigens maximally sensitized with rabbit sera have shown isoelectric points of pH 5.6 to 5.8. The globulins precipitated or salted out of the same immune sera have been isoelectric at pH 5.1 to 5.2. The combination of antigen with antibody depends of course upon specific affinities; the surface properties of the sensitized antigen, agglutination and phagocytosis depend primarily upon the properties of the sensitizing serum substances combined with and deposited on the antigen surface.


1921 ◽  
Vol 3 (3) ◽  
pp. 309-323 ◽  
Author(s):  
Calvin B. Coulter

1. The movement of normal and sensitized red blood cells in the electric field is a function of the hydrogen ion concentration. The isoelectric point, at which no movement occurs, corresponds with pH 4.6. 2. On the alkaline side of the isoelectric point the charge carried is negative and increases with the alkalinity. On the acid side the charge is positive and increases with the acidity. 3. On the alkaline side at least the charge carried by sensitized cells is smaller and increases less rapidly with the alkalinity than the charge of normal cells. 4. Both normal and sensitized cells combine chemically with inorganic ions, and the isoelectric point is a turning point for this chemical behavior. On the acid side the cells combine with the hydrogen and chlorine ions, and in much larger amount than on the alkaline side; on the alkaline side the cells combine with a cation (Ba), and in larger amount than on the acid side. This behavior corresponds with that found by Loeb for gelatin. 5. The optimum for agglutination of normal cells is at pH 4.75, so that at this point the cells exist most nearly pure, or least combined with anion and cation. 6. The optimum for agglutination of sensitized cells is at pH 5.3. This point is probably connected with the optimum for flocculation of the immune serum body.


1982 ◽  
Vol 58 (1) ◽  
pp. 1-22
Author(s):  
R.W. Linck ◽  
G.L. Langevin

By progressive solvent extraction, we have obtained a series of subfragments of flagellar microtubules. Mild treatment gives rise to ribbons that contain longitudinally arranged protofilaments. Further extraction leaves a distinctive residue containing thinner ribbons, of three and eventually two protofilaments. Finally, filaments 2–3 nm in diameter and fibrous ribbons apparently containing 6 or more 2 nm subfibrils are found. This latter solvent-resistant material is consistently enriched in a characteristic set of polypeptides, which are found in flagella of several different species, including echinoderms and a mollusc. These polypeptides appear different from alpha- and beta-tubulin on the basis of their solubilities, isoelectric points and electrophoretic mobilities in sodium dodecyl sulphate/polyacrylamide gels; these conclusions are reinforced by peptide mapping after limited proteolytic digestion, although the latter method reveals certain similarities between these unique flagellar proteins, tubulin, chicken gizzard desmin and rabbit actin. A remarkable feature of the protein in the final fraction is the high alpha-helical content: 71% as measured by circular dichroism. We consider the possible origins of these filaments in the microtubule, in particular the possibility that microtubule protofilaments are heterogeneous in protein composition, and we discuss some of the implications of our findings.


2018 ◽  
Vol 4 (2) ◽  
pp. 61 ◽  
Author(s):  
Isabel Martínez ◽  
Ángel Castillo

Resumen El hipódromo de la Zarzuela, declarado patrimonio histórico nacional, fue construido en Madrid en los años 40, y es una obra del Ingeniero Eduardo Torroja y los arquitectos Arniches y Domínguez. Las cubiertas de su estructura son consideradas un arte desde el punto de vista ingenieril. A pesar del perfecto y genial equilibrio físico y mecánico, se iniciaron procesos físicos y físico-químicos de deterioro en su composición debido al largo periodo de exposición de la estructura a la acción climática y al ataque de los agentes agresivos atmosféricos, entre los cuales se destaca como principal patología la corrosión por carbonatación de su armadura embebida. Dada la importancia de la obra desde el punto de vista artístico, histórico y de seguridad, las autoridades correspondientes decidieron su rehabilitación. En este proyecto de reparación se contempló la instalación de un sistema de monitorización de medidas continuas, mediante sensores electroquímicos pasivos, capaces de indicar el riesgo de corrosión en la armadura embebida de la estructura. De este modo, es posible controlar el comportamiento del material frente a la interacción con la acción atmosférica y prever la posible necesidad de intervenciones de mantenimiento, evitando el avance del deterioro con rapidez y garantías. La implementación de la monitorización requirió de una evaluación mediante técnicas electroquímicas no-destructivas del grado de deterioro previo a la intervención, para poder estudiar la evolución del comportamiento de la estructura después de la reparación. En el presente trabajo, se presentan los resultados obtenidos tanto en la evaluación electroquímica previa en las cubiertas del Hipódromo de la Zarzuela, como su posterior monitorización durante casi 10 años. Se presenta también la viabilidad del sistema de monitorización y su fiabilidad con el tiempo. Abstract La Zarzuela racecourse, declared a national historical heritage, was built in Madrid in the 1940s, and is a work by EngineerEduardo Torroja and the architects Arniches and Domínguez. The covers of its structure are considered an art from the engineeringpoint of view. Despite the perfect and ingenious physical and mechanical balance, physical and physical-chemical processes ofdeterioration in its composition were initiated due to the long period of exposure of the structure to climatic action and the attack ofaggressive atmospheric agents, among which the main pathology is the corrosion by carbonation of its embedded armor. Given theimportance of the work from the artistic, historical and security point of view, the corresponding authorities decided on itsrehabilitation. In this repair project, the installation of a continuous measurement monitoring system was contemplated, using passiveelectrochemical sensors, capable of indicating the risk of corrosion in the structure's embedded reinforcement. In this way, it is possibleto control the behavior of the material against the interaction with atmospheric action and to foresee the possible need for maintenanceinterventions, avoiding the advance of deterioration with speed and guarantees. The implementation of the monitoring required anevaluation by non-destructive electrochemical techniques of the degree of deterioration prior to the intervention, in order to study theevolution of the behavior of the structure after the repair. In the present work, the results obtained in the previous electrochemicalevaluation on the decks of the Hipódromo de la Zarzuela are presented, as well as their subsequent monitoring for almost 10 years.The viability of the monitoring system and its reliability over time are also presented.


1971 ◽  
Vol 133 (5) ◽  
pp. 1090-1104 ◽  
Author(s):  
R. V. Blanden

Histological and immunofluorescence techniques showed that mononuclear cells invaded virus-infected foci in the livers of passively immunized mice within 10 hr of the receipt of immune spleen cells or hyperimmune serum; by 24 hr, marked destruction of virus antigens had occurred in these lesions. Immune cell transfer promoted denser packing of mononuclear cells in the foci and more efficient destruction of infectious material than immune serum. Similar liver lesions developed by the 6th day after sublethal, primary, subcutaneous infection in normal mice. In contrast, in mice with GVHR which were immunosuppressed but possessed hyperactive macrophages and unimpaired splenic interferon response, mononuclear cells did not invade liver lesions and the animals died. These results, together with data reported previously, indicated that mononuclear cell invasion of infected liver foci, triggered by CMI, was of key importance in recovery from primary mousepox. The roles of specifically sensitized lymphocytes and macrophages within lesions were not directly evaluated, but indirect evidence suggested that lymphocytes could cause no more than a halt in virus multiplication, and that macrophages were required for the inactivation of preformed virions. Possible augmentation of the efficiency of macrophages by locally-produced lymphocyte interferon, neutralizing antibody, or stimulation of their phagocytic and intracellular digestive capacity cannot be excluded.


1932 ◽  
Vol 56 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Lloyd D. Felton

From the study of different tissue extracts as media for the growth of pneumococci used in an automatic transfer device, certain inferences are warranted: 1. Media made from calf lung or heart, or from horse skeletal muscle maintain virulence over a long period of time. Conversely, media made from calf spleen lead to a decrease in virulence. 2. Lung medium causes an increase in virulence of seven strains of pneumococci. 3. Virulence is maintained in normal horse serum; but, it rapidly decreases in immune serum, or in pneumococcus antibody solution, a finding which confirms the work of Stryker. Immune serum freed from protective antibody gives results similar to normal serum. 4. Rabbit medium made from the entire animal apparently is less suitable for the maintenance of virulence of pneumococci than medium made in the same way from guinea pig.


1961 ◽  
Vol 114 (1) ◽  
pp. 111-125 ◽  
Author(s):  
William O. Weigle

Immunological tolerance produced in rabbits by neonatal injections of BSA can be terminated by a series of injections of certain heterologous serum albumins which cross-react with BSA. Injections of albumins distantly related to BSA were more effective in terminating the tolerant state than injections of albumins closely related to BSA. It was concluded from results obtained with several heterologous albumins that immunological tolerance to BSA is directed to both the over-all antigenic or physical-chemical composition of the protein and the individual determinant groups present on the protein. Several possible mechanisms were given to explain the ability of cross-reacting albumins to terminate the tolerant state of BSA-tolerant rabbits. A possible relationship between the termination of tolerance in BSA-tolerant rabbits injected with cross-reacting albumins and autoimmunily was discussed. It was also suggested that the relative ease with which tolerance could be established to heterologous serum proteins in comparison to bacterial antigens is the result of the close serological and physical-chemical relationship of the heterologous serum proteins to the serum proteins of the rabbit. Rabbits injected with 500 mg of BSA during the first 5 days of life failed to form antibody capable of either eliciting an immune elimination of an injection of I* BSA given 3 to 4 months later or complexing with the circulating I* BSA.


2016 ◽  
Vol 12 (30) ◽  
pp. 127
Author(s):  
Mohammed Izougarhane ◽  
Dalale Mansouri ◽  
Hamid El Ibaoui1 ◽  
Said Chakiri ◽  
Mohamed Fadli

This work focuses on the physical, chemical and metal characterization of the estuary waters of Oued Sebou during a long period of sand dredging. To do this, several physicochemical parameters were evaluated during periods 2007 and 2014-2016. The results showed that this estuary has a significant degree of pollution. The source of this pollution is diverse: urban waste, industrial waste, agricultural activities. This pollution is accentuated by the action of repeated dredging that increases the turbidity and the suspended solids content which covers the seabed during immersions that are covering the sediment and disturbs the physicochemical equilibrium of the estuary. So, indirectly this operation of dredging influences the parameters that are related to the amount and quality of materials suspended such as the dissolved oxygen content and orthophosphates and the brightness level in the water and over up the sediment.Indeed, for the majority of the physicochemical parameters assessed, the water of the estuary was belonging to a middle class, poor class or very poor class. Thus, the operation of dredging should consider the ecological balance of the coastal zone. Note also that the contents of assessed heavy metals are not worrisome. However, their risks might be amplified by the dredging operations.


2021 ◽  
Vol 24 ◽  
Author(s):  
Maritiele Naissinger da Silva ◽  
Bruna Lago Tagliapietra ◽  
Franciele Pozzebon Pivetta ◽  
Vinicius do Amaral Flores ◽  
Neila Silvia Pereira dos Santos Richards

Abstract The consumption and consequent production of butter has increased considerably in recent years. In order to know the butters sold in Brazil, the study aimed to analyze butters of Brazilian, French, Italian and Argentine origin. The samples were analyzed for fat content, moisture, Defatted Dry Extract (DDE), and total acidity for comparison with Brazilian legislation. The levels of chlorides, protein, ash, total dry extract, water activity, color and determination of the lipid profile were also determined. In all analyses, there was a statistically significant difference between the samples. Some samples showed disagreement with the quality requirements recommended in Brazilian legislation for commercialization. Indeed, 10 samples did not present the minimum fat content required, varying from 68.53% to 77.31% in butters with salt and from 71.64% to 81.72% in those without salt. Eight samples presented humidity levels above the legal recommendations, varying from 17.05% to 20.28%. All products were in agreement with acidity levels. The predominant fatty acids in all samples were myristic acid (C14: 0), palmitic acid (C16: 0), stearic acid (C18: 0) and oleic acid (C18: 1n9). The regional influence, breed and handling of the animals and season of the year in which the milk was obtained for butter production, were suggested to be the main reason for the physical-chemical difference found between the samples.


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