scholarly journals PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

1954 ◽  
Vol 99 (2) ◽  
pp. 167-182 ◽  
Author(s):  
R. Dulbecco ◽  
Marguerite Vogt
Keyword(s):  
Virus Particle ◽  
Parent Strain ◽  
High Titer ◽  
Plaque Formation ◽  
Monkey Kidney ◽  
Specific Antiserum ◽  
Tissue Cultures ◽  
Virus Population ◽  
Pure Lines

Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 108 plaque-forming particles per ml., were obtained.

scholarly journals DOUBLE INFECTIONS OF SINGLE CELLS WITH ECHO 7 AND COXSACKIE A9 VIRUSES

1959 ◽  
Vol 109 (4) ◽  
pp. 393-406 ◽  
Author(s):  
Heihachi Itoh ◽  
Joseph L. Melnick
Keyword(s):  
Rhesus Monkey ◽  
Parent Strain ◽  
Single Cells ◽  
Growth Cycle ◽  
Monkey Kidney ◽  
Monkey Kidney Cell ◽  
Newborn Mice ◽  
Virus Particles ◽  
Infected Cells ◽  
Number Of Cells

Virus particles derived from single cells infected with two enteroviruses have been studied. Evidence was obtained to indicate that phenotypic, but not genotypic, mixing occurs between Coxsackie A9 (CAP) and ECHO 7 (E7) viruses. Monkey kidney cultures in monolayer were doubly infected with high multiplicities of CA9 and E7 viruses. During the latent period, the infected cells were suspended, diluted, and distributed under oil into droplets each containing a single cell, as checked by microscopic observation. The virus particles released by individual cells into the microdrop were characterized in differential plaque neutralization tests. Fifteen per cent of the microdrops contained doubly neutralizable particles, 53 per cent yielded either CA9 or E7 particles, and 34 yielded particles of an intermediate character (deficits between 37 and 75 per cent). On passage, the doubly neutralizable particles yielded progeny of both parental types. All passage strains behaved like the corresponding parent strain as regards pathogenicity for newborn mice, which is to say that this property was limited to virus particles with CA9 antigenicity. Coxsackie A9 has a more rapid growth cycle than ECHO 7 in rhesus monkey kidney cell cultures, and a slower one in patas cultures. In rhesus, when E7 virus was added first, CA9 could be added up to 2 hours later, and still a significant number of cells yielded either CA9 or doubly neutralizable virus. The converse was observed in patas cells.

scholarly journals THE ROLE OF METHIONINE DEFICIENCY IN POLIOVIRUS REPLICATION IN TISSUE CULTURES

1966 ◽  
Vol 123 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Soussan Mohajer ◽  
Janis Gabliks
Keyword(s):  
Amino Acids ◽  
Hela Cells ◽  
Inhibitory Effect ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Experimental Conditions ◽  
Methionine Deficiency ◽  
Intracellular Amino Acids

The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10–3 and 4.0 x 10–3 moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.

Plaque formation by influenza A2 virus in monkey kidney cells

Virology ◽  
1962 ◽  
Vol 18 (2) ◽  
pp. 332-334 ◽  
Author(s):  
Purnell W. Choppin
Keyword(s):  
Plaque Formation ◽  
Monkey Kidney ◽  
Kidney Cells

A RABIES VACCINE FROM HAMSTER KIDNEY TISSUE CULTURES: PREPARATION AND EVALUATION IN ANIMALS

1960 ◽  
Vol 6 (6) ◽  
pp. 605-609 ◽  
Author(s):  
Paul Fenje
Keyword(s):  
Salivary Glands ◽  
Rabies Virus ◽  
High Titer ◽  
Kidney Tissue ◽  
Rabies Vaccine ◽  
Tissue Cultures ◽  
Intramuscular Inoculation ◽  
Tissue Cells ◽  
Preparation And Evaluation ◽  
High Infectivity

A strain of fixed rabies virus adapted to hamster kidney tissue cells has produced culture fluids of high infectivity for rabbits and mice. These culture fluids were rendered non-infective by treatment with formaldehyde at a concentration of 8 m M. Rabbits immunized with this material produced autirabies antibody to a high titer and were subsequently proved to be resistant to intramuscular inoculation of rabies virus from the salivary glands of a naturally infected fox.

Infection of Monkey Kidney Tissue Cultures with Virus-Like Agents.

1955 ◽  
Vol 88 (1) ◽  
pp. 8-16 ◽  
Author(s):  
R. Rustigian ◽  
P. Johnston ◽  
H. Reihart
Keyword(s):  
Kidney Tissue ◽  
Monkey Kidney ◽  
Tissue Cultures

scholarly journals An outbreak of aseptic meningitis associated with a previously unrecognized virus

1961 ◽  
Vol 59 (2) ◽  
pp. 181-189 ◽  
Author(s):  
I. B. R. Duncan
Keyword(s):  
Aseptic Meningitis ◽  
Kidney Tissue ◽  
Monkey Kidney ◽  
Human Thyroid ◽  
Tissue Cultures ◽  
Human Amnion ◽  
Echo Virus

In 1959, 69 cases of aseptic meningitis were admitted to various hospitals in Scotland—all apparently due to a Hitherto unrecognized virus. This agent had the characteristics of an ECHO virus but differed from the 28 ECHO viruses at present recognized. Seventy-five strains of the virus were isolated, and human thyroid and human amnion tissue cultures proved much superior to monkey kidney tissue cultures for its isolation.

Serial Propagation of Adenoviruses (APG) in Monkey Kidney Tissue Cultures

1956 ◽  
Vol 92 (4) ◽  
pp. 667-669 ◽  
Author(s):  
J. W. Hartley ◽  
R. J. Huebner ◽  
W. P. Rowe
Keyword(s):  
Kidney Tissue ◽  
Monkey Kidney ◽  
Tissue Cultures ◽  
Serial Propagation
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