scholarly journals Selective use of the VHQ52 family in functional VH to DJH rearrangements in a B precursor cell line.

1987 ◽  
Vol 166 (2) ◽  
pp. 607-612 ◽  
Author(s):  
H Sugiyama ◽  
T Maeda ◽  
Y Tani ◽  
S Miyake ◽  
Y Oka ◽  
...  
Keyword(s):  
Cell Line ◽  
Variable Region ◽  
Gene Families ◽  
Precursor Cell ◽  
Transformed Cell Line ◽  
Precursor Cell Line ◽  
Functional Variable ◽  
Vh Gene ◽  
Abelson Virus ◽  
Variable Region Genes

AT11-2, an Abelson virus-transformed cell line has DJH complexes on both chromosomes and is able to form functional variable region genes by the joins of VH genes to the DJH complexes during culture. Therefore we examined which VH gene family was used in functional VH to DJH recombinations in AT11-2. Surprisingly, of 32 independent functional VH to DJH recombinational events in AT11-2, 31 events used the VH segments of the VHQ52 family, and the remaining one used the VH segment of the VH7183 family. Thus, we describe here the first B precursor cell line that almost selectively uses the VHQ52 family in functional VH to DJH rearrangements. The selective use of the VHQ52 family in this B precursor cell line strongly indicates nonrandom use of VH gene families, and the existence of a stage at which the VHQ52 family is preferentially used during the normal development of early pre-B cells and has important implications for understanding the ontogeny of VH repertoire development. Furthermore, this cell line should prove extremely valuable in further studies of this kind.

Production of laminin B2 chain protein and messenger RNA by a murine neutrophil precursor cell line, 32Dc13 [see comments]

Blood ◽  
1990 ◽  
Vol 76 (7) ◽  
pp. 1398-1404 ◽  
Author(s):  
DJ Tweardy ◽  
M Sasaki ◽  
JJ Jr Cardamone ◽  
JP Jr McCoy ◽  
MJ Bonidie ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Line ◽  
Messenger Rna ◽  
Precursor Cell ◽  
Laminin Receptors ◽  
Precursor Cell Line ◽  
Myeloid Development ◽  
Exogenous Origin ◽  
And Function ◽  
Murine Neutrophil

Laminin is a heterotrimeric glycoprotein that plays a central role in promoting neutrophil chemotaxis, motility, and attachment to basement membrane. Rabbit peritoneal exudate neutrophils stain positively for laminin, which is presumed to be of exogenous origin and bound to laminin receptors on the cell surface. We examined 32Dc13 cells, a murine neutrophil precursor cell line, by immunoprecipitation. Northern blot analysis, flow cytometry, and electron microscopy for the endogenous production of laminin. Our results demonstrate that 32Dc13 cells endogenously produce a laminin B2 chain protein and messenger RNA (mRNA) without producing any detectable A or B1 chain protein or mRNA. The B2 chain protein was not secreted by the cells; rather it could be detected on the cell surface after treatment of cells with neuraminidase. These findings suggest the possibility of a novel role for the laminin B2 chain in myeloid development and function.

cAMP-mediated growth inhibition of a B-lymphoid precursor cell line Reh is associated with an early transient delay in G2/M, followed by an accumulation of cells in G1

1988 ◽  
Vol 137 (3) ◽  
pp. 583-587 ◽  
Author(s):  
Heidi Kiil Blomhoff ◽  
Rune Blomhoff ◽  
Trond Stokke ◽  
Catharina DeLange Davies ◽  
Kristin Brevik ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Cell Line ◽  
Precursor Cell ◽  
Precursor Cell Line ◽  
B Lymphoid

Unmutated Ig VH Genes Are Associated With a More Aggressive Form of Chronic Lymphocytic Leukemia

Blood ◽  
1999 ◽  
Vol 94 (6) ◽  
pp. 1848-1854 ◽  
Author(s):  
Terry J. Hamblin ◽  
Zadie Davis ◽  
Anne Gardiner ◽  
David G. Oscier ◽  
Freda K. Stevenson
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
B Cell ◽  
Somatic Mutation ◽  
Variable Region ◽  
Lymphocytic Leukemia ◽  
Cell Of Origin ◽  
Trisomy 12 ◽  
Vh Gene ◽  
Vh Genes ◽  
Variable Region Genes

Abstract Despite having several characteristics of naı̈ve B cells, chronic lymphocytic leukemia (CLL) cells have been shown in some cases to have somatically mutated Ig variable region genes, indicating that the cell of origin has passed through the germinal center. A previous study of patients with CLL found an association between lack of somatic mutation and trisomy 12 and, therefore, possibly with a less favorable prognosis. We have sequenced the Ig VH genes of the tumor cells of 84 patients with CLL and correlated our findings with clinical features. A total of 38 cases (45.2%) showed ≥ 98% sequence homology with the nearest germline VH gene; 46 cases (54.8%) showed >2% somatic mutation. Unmutated VH genes were significantly associated with V1-69 and D3-3 usage, with atypical morphology; isolated trisomy 12, advanced stage and progressive disease. Survival was significantly worse for patients with unmutated VH genes irrespective of stage. Median survival for stage A patients with unmutated VH genes was 95 months compared with 293 months for patients whose tumors had mutated VHgenes (P = .0008). The simplest explanation is that CLL comprises 2 different diseases with different clinical courses. One, arising from a memory B cell, has a benign course, the other, arising from a naı̈ve B cell, is more malignant.

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