scholarly journals Exploring Tissue Engineering: Vitamin D3 Influences on the Proliferation and Differentiation of an Engineered Osteoblast Precursor Cell Line During Early Bone Tissue Development

2000 ◽  
Author(s):  
Shelley Mason
Keyword(s):  
Tissue Engineering ◽  
Cell Line ◽  
Bone Tissue ◽  
Vitamin D3 ◽  
Precursor Cell ◽  
Tissue Development ◽  
Precursor Cell Line ◽  
Proliferation And Differentiation ◽  
Osteoblast Precursor Cell

An in vitro osteoblast culture model using estrogen responsive Ks483 mouse osteoblast precursor cell line

Bone ◽  
2010 ◽  
Vol 47 ◽  
pp. S120
Author(s):  
K.M. Fagerlund ◽  
J.P. Rissanen ◽  
T. Suutari ◽  
A. Chan ◽  
J.M. Halleen
Keyword(s):  
Cell Line ◽  
Precursor Cell ◽  
Osteoblast Culture ◽  
Precursor Cell Line ◽  
Culture Model ◽  
Mouse Osteoblast ◽  
Osteoblast Precursor Cell

Fabrication and characterization of novel ethyl cellulose-grafted-poly (ɛ-caprolactone)/alginate nanofibrous/macroporous scaffolds incorporated with nano-hydroxyapatite for bone tissue engineering

2019 ◽  
Vol 33 (8) ◽  
pp. 1128-1144 ◽  
Author(s):  
Vahideh Raeisdasteh Hokmabad ◽  
Soodabeh Davaran ◽  
Marziyeh Aghazadeh ◽  
Reza Rahbarghazi ◽  
Roya Salehi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Ethyl Cellulose ◽  
Three Dimensional ◽  
Drying Methods ◽  
Layer By Layer ◽  
Freeze Dried ◽  
Proliferation And Differentiation

The major challenge of tissue regeneration is to develop three dimensional scaffolds with suitable properties which would mimic the natural extracellular matrix to induce the adhesion, proliferation, and differentiation of cells. Several materials have been used for the preparation of the scaffolds for bone regeneration. In this study, novel ethyl cellulose-grafted-poly (ɛ-caprolactone) (EC-g-PCL)/alginate scaffolds with different contents of nano-hydroxyapatite were prepared by combining electrospinning and freeze-drying methods in order to provide nanofibrous/macroporous structures with good mechanical properties. For this aim, EC-g-PCL nanofibers were obtained with electrospinning, embedded layer-by-layer in alginate solutions containing nano-hydroxyapatite particles, and finally, these constructions were freeze-dried. The scaffolds possess highly porous structures with interconnected pore network. The swelling, porosity, and degradation characteristics of the EC-g-PCL/alginate scaffolds were decreased with the increase in nano-hydroxyapatite contents, whereas increases in the in-vitro biomineralization and mechanical strength were observed as the nano-hydroxyapatite content was increased. The cell response to EC-g-PCL/alginate scaffolds with/or without nano-hydroxyapatite was investigated using human dental pulp stem cells (hDPSCs). hDPSCs displayed a high adhesion, proliferation, and differentiation on nano-hydroxyapatite-incorporated EC-g-PCL/alginate scaffolds compared to pristine EC-g-PCL/alginate scaffold. Overall, these results suggested that the EC-g-PCL/alginate-HA scaffolds might have potential applications in bone tissue engineering.

Bone tissue engineering with scaffold-supported perfusion co-cultures of human stem cell-derived osteoblasts and cell line-derived osteoclasts

2017 ◽  
Vol 59 ◽  
pp. 303-311 ◽  
Author(s):  
Işıl G. Beşkardeş ◽  
Rebecca S. Hayden ◽  
Dean L. Glettig ◽  
David L. Kaplan ◽  
Menemşe Gümüşderelioğlu
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Cell Line ◽  
Bone Tissue ◽  
Human Stem Cell

Development of new graphene oxide incorporated tricomponent scaffolds with polysaccharides and hydroxyapatite and study of their osteoconductivity on MG-63 cell line for bone tissue engineering

RSC Advances ◽  
2015 ◽  
Vol 5 (51) ◽  
pp. 41135-41143 ◽  
Author(s):  
R. Rajesh ◽  
Y. Dominic Ravichandran
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Bone Tissue Engineering ◽  
Cell Line ◽  
Bone Tissue ◽  
Gellan Gum ◽  
First Time

GO–alginate–HAP, GO–amylopectin–HAP and GO–gellan gum–HAP were prepared and characterized and their osteoconductivity were checked for the first time.

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