scholarly journals Characterization of human rheumatoid factors with seven antiidiotypes induced by synthetic hypervariable region peptides.

1985 ◽  
Vol 162 (2) ◽  
pp. 487-500 ◽  
Author(s):  
P P Chen ◽  
F Gõni ◽  
R A Houghten ◽  
S Fong ◽  
R Goldfien ◽  
...  
Keyword(s):  
Rheumatoid Factor ◽  
Synthetic Peptides ◽  
Hypervariable Region ◽  
Rheumatoid Factors ◽  
Immunoblot Assay ◽  
The Third ◽  
Antiidiotypic Antibodies ◽  
Vh Genes ◽  
Complementarity Determining Regions

Recently, we have used synthetic peptides corresponding to the complementarity-determining regions (CDR) of Ig molecules to induce antiidiotypic antisera. Peptide PSH3, representing the third CDR of the IgM rheumatoid factor (RF) Sie heavy (H) chain, induced a private antiidiotype that reacted with only one out of five IgM-RF. Peptide PSL2, corresponding to the second CDR of Sie light (L) chain, induced an antibody against a crossreactive idiotype (CRI), expressed by 10 out of 12 human IgM-RF analyzed. Herein, we report that five additional antiidiotypic antibodies were generated by immunization with synthetic peptides identical to the third L chain CDR of IgM-RF Sie (PSL3), the second and third H chain CDR of IgM-RF Wol, and the second and third CDR of IgM-RF Pom. As analyzed by immunoblot assay, both anti-PSL3 and anti-PSL2 reacted with the majority of 16 IgM-RF. In contrast, all five antiidiotypes induced by the H chain peptides reacted only with the parent proteins, except anti-PSH3, which reacted weakly with one additional RF. These results suggest that one (or very few) VL gene(s), but a larger number of VH genes, are used to encode IgM-RF autoantibodies.

scholarly journals Synthetic peptides corresponding to third hypervariable region of human monoclonal IgM rheumatoid factor heavy chains define an immunodominant idiotype.

1985 ◽  
Vol 162 (2) ◽  
pp. 756-761 ◽  
Author(s):  
R D Goldfien ◽  
P P Chen ◽  
S Fong ◽  
D A Carson
Keyword(s):  
Rheumatoid Factor ◽  
Heavy Chain ◽  
Synthetic Peptides ◽  
Gene Segment ◽  
Hypervariable Region ◽  
Heavy Chains ◽  
The Third ◽  
Specific Induction ◽  
Antiidiotypic Antibodies ◽  
Complementarity Determining Regions

Synthetic peptides corresponding to eight individual heavy chain complementarity-determining regions (CDR) of three human monoclonal IgM anti-IgG (rheumatoid factor [RF]) paraproteins elicited rabbit antibodies with markedly different properties. All antisera recognized the immunizing peptide, and several reacted with the isolated IgM heavy chain on immunoblots. However, only the antisera against peptides representing the third CDR bound consistently and specifically to the intact IgM-RF molecule. These data indicate that the third CDR of human mu chains comprises an immunodominant idiotype, and suggest that the D gene segment may be especially important in creating idiotypic diversity. Synthetic peptides corresponding to the third heavy chain CDR of human paraproteins may be clinically useful for the specific induction of antiidiotypic antibodies.

scholarly journals Structural characterization of antiidiotypic antibodies. Evidence that Ab2s are derived from the germline differently than Ab1s.

1989 ◽  
Vol 169 (2) ◽  
pp. 519-533 ◽  
Author(s):  
K Meek ◽  
C Hasemann ◽  
B Pollok ◽  
S S Alkan ◽  
M Brait ◽  
...  
Keyword(s):  
Hypervariable Region ◽  
Variable Region ◽  
Good Evidence ◽  
Foreign Protein ◽  
Germline Gene ◽  
Region Gene ◽  
The Third ◽  
Antiidiotypic Antibodies ◽  
V Region

We have found that syngeneic Ab2s in the antiarsonate system are serologically and structurally similar to one another. In contrast, the allogeneic Ab2 response is heterogeneous and derives from a large number of unrelated germline gene segments. The Ab2 response of the BALB/c strain to polyclonal A/J Ars A molecules can probably best be compared with a response to a foreign protein and might have been predicted in a strain that completely lacks the H chain V region gene from which the Ab1 derives. Partial variable region sequences of Ab2s from three other systems in addition to previously reported Ab2 structures indicates that this difference in allogeneic vs. syngeneic Ab2s may be a general phenomena. These data support Jerne's hypothesis of complementary V region genes existing in the germline. However, there is good evidence that these antiidiotypic antibodies are not derived directly from the germline, as somatic processes most likely play an important role in their generation. The D segments of Ab2s in the arsonate system as well as in other systems, are novel in structure and cannot easily be explained by previously described germline D segments. D-D fusion may play a role in the generation of the third hypervariable region in these antibodies.

scholarly journals Balancing sensitivity and specificity in distinguishing TCR groups by CDR sequence similarity

2019 ◽  
Author(s):  
Neerja Thakkar ◽  
Chris Bailey-Kellogg
Keyword(s):  
Predictive Power ◽  
Sequence Similarity ◽  
Structural Similarity ◽  
Cell Receptor ◽  
Antigen Specificity ◽  
The Third ◽  
Repertoire Sequencing ◽  
Complementarity Determining Regions ◽  
Cellular Immune

AbstractRepertoire sequencing is enabling deep explorations into the cellular immune response, including the characterization of commonalities and differences among T cell receptor (TCR) repertoires from different individuals, pathologies, and antigen specificities. In seeking to understand the generality of patterns observed in different groups of TCRs, it is necessary to balance how well each pattern represents the diversity among TCRs from one group (sensitivity) vs. how many TCRs from other groups it also represents (specificity). The variable complementarity determining regions (CDRs), particularly the third CDRs (CDR3s) interact with MHC-presented epitopes from putative antigens, and thus encode the determinants of recognition. We here systematically characterize the predictive power that can be obtained from CDR3 sequences, using representative, readily interpretable methods for evaluating CDR sequence similarity and then clustering and classifying sequences based on similarity. An initial analysis of CDR3s of known structure, clustered by structural similarity, helps calibrate the limits of sequence diversity among CDRs that might have a common mode of interaction with presented epitopes. Subsequent analyses demonstrate that this same range of sequence similarity strikes an appropriate specificity/sensitivity balance in distinguishing twins from non-twins based on overall CDR3 repertoires, classifying CDR3 repertoires by antigen specificity, and distinguishing general pathologies. We conclude that within this fairly broad range of sequence similarity, matching CDR3 sequences are likely to share specificities.

scholarly journals Characterization of two immunoglobulin VH genes that are homologous to human rheumatoid factors

1989 ◽  
Vol 32 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Pojen P. Chen ◽  
Ming-Fei Liu ◽  
Charles A. Glass ◽  
Suman Sinha ◽  
Thomas J. Kipps ◽  
...  
Keyword(s):  
Rheumatoid Factors ◽  
Vh Genes

scholarly journals Characterization of an epibody. An antiidiotype that reacts with both the idiotype of rheumatoid factors (RF) and the antigen recognized by RF.

1985 ◽  
Vol 161 (2) ◽  
pp. 323-331 ◽  
Author(s):  
P P Chen ◽  
S Fong ◽  
R A Houghten ◽  
D A Carson
Keyword(s):  
Synthetic Peptide ◽  
Molecular Basis ◽  
Hypervariable Region ◽  
Rheumatoid Factors ◽  
Human Igg

Recently, an antiidiotype to human monoclonal IgM anti-IgG autoantibodies (rheumatoid factors) was found to react also with human IgG. This peculiar antiidiotype was called an 'epibody'. We describe the induction of a similar epibody by immunization with a synthetic peptide (corresponding to one hypervariable region of the IgM-RF Glo). The results confirm the existence of epibodies, and provide the possible molecular basis of the epibody phenomenon.

Gaunilo Parodies Anselm

2014 ◽  
Vol 17 (1) ◽  
pp. 45-71
Author(s):  
Geo Siegwart
Keyword(s):  
Detailed Comparison ◽  
The Third ◽  
Common Structure ◽  
Logical Reconstruction ◽  
Points Of View ◽  
And Function

The main objective is an interpretation of the island parody, in particular a logical reconstruction of the parodying argument that stays close to the text. The parodied reasoning is identified as the proof in the second chapter of the Proslogion, more specifically, this proof as it is represented by Gaunilo in the first chapter of his Liber pro insipiente. The second task is a detailed comparison between parodied and parodying argument as well as an account of their common structure. The third objective is a tentative characterization of the nature and function of parodies of arguments. It seems that parodying does not add new pertinent points of view to the usual criticism of an argument.

Kinetic Characterization of Anticarsia gemmatalis Digestive Serine- Proteases and the Inhibitory Effect of Synthetic Peptides

2018 ◽  
Vol 24 (11) ◽  
Author(s):  
Adriana M. Patarroyo-Vargas ◽  
Yaremis B. Merino-Cabrera ◽  
Jose C. Zanuncio ◽  
Francelina Rocha ◽  
Wellington G. Campos ◽  
...  
Keyword(s):  
Synthetic Peptides ◽  
Serine Proteases ◽  
Inhibitory Effect ◽  
Anticarsia Gemmatalis ◽  
Kinetic Characterization

scholarly journals A discontinuous factor H binding site in the third component of complement as delineated by synthetic peptides.

1988 ◽  
Vol 263 (24) ◽  
pp. 12147-12150 ◽  
Author(s):  
J D Lambris ◽  
D Avila ◽  
J D Becherer ◽  
H J Müller-Eberhard
Keyword(s):  
Binding Site ◽  
Synthetic Peptides ◽  
Factor H ◽  
The Third ◽  
Third Component Of Complement
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