scholarly journals Characterization of an epibody. An antiidiotype that reacts with both the idiotype of rheumatoid factors (RF) and the antigen recognized by RF.

1985 ◽  
Vol 161 (2) ◽  
pp. 323-331 ◽  
Author(s):  
P P Chen ◽  
S Fong ◽  
R A Houghten ◽  
D A Carson
Keyword(s):  
Synthetic Peptide ◽  
Molecular Basis ◽  
Hypervariable Region ◽  
Rheumatoid Factors ◽  
Human Igg

Recently, an antiidiotype to human monoclonal IgM anti-IgG autoantibodies (rheumatoid factors) was found to react also with human IgG. This peculiar antiidiotype was called an 'epibody'. We describe the induction of a similar epibody by immunization with a synthetic peptide (corresponding to one hypervariable region of the IgM-RF Glo). The results confirm the existence of epibodies, and provide the possible molecular basis of the epibody phenomenon.

scholarly journals Characterization of human rheumatoid factors with seven antiidiotypes induced by synthetic hypervariable region peptides.

1985 ◽  
Vol 162 (2) ◽  
pp. 487-500 ◽  
Author(s):  
P P Chen ◽  
F Gõni ◽  
R A Houghten ◽  
S Fong ◽  
R Goldfien ◽  
...  
Keyword(s):  
Rheumatoid Factor ◽  
Synthetic Peptides ◽  
Hypervariable Region ◽  
Rheumatoid Factors ◽  
Immunoblot Assay ◽  
The Third ◽  
Antiidiotypic Antibodies ◽  
Vh Genes ◽  
Complementarity Determining Regions

Recently, we have used synthetic peptides corresponding to the complementarity-determining regions (CDR) of Ig molecules to induce antiidiotypic antisera. Peptide PSH3, representing the third CDR of the IgM rheumatoid factor (RF) Sie heavy (H) chain, induced a private antiidiotype that reacted with only one out of five IgM-RF. Peptide PSL2, corresponding to the second CDR of Sie light (L) chain, induced an antibody against a crossreactive idiotype (CRI), expressed by 10 out of 12 human IgM-RF analyzed. Herein, we report that five additional antiidiotypic antibodies were generated by immunization with synthetic peptides identical to the third L chain CDR of IgM-RF Sie (PSL3), the second and third H chain CDR of IgM-RF Wol, and the second and third CDR of IgM-RF Pom. As analyzed by immunoblot assay, both anti-PSL3 and anti-PSL2 reacted with the majority of 16 IgM-RF. In contrast, all five antiidiotypes induced by the H chain peptides reacted only with the parent proteins, except anti-PSH3, which reacted weakly with one additional RF. These results suggest that one (or very few) VL gene(s), but a larger number of VH genes, are used to encode IgM-RF autoantibodies.

Characterization of class II fumarase from Schistosoma mansoni provides the molecular basis for selective inhibition

2021 ◽  
Vol 175 ◽  
pp. 406-421
Author(s):  
Iara Aimê Cardoso ◽  
Aline Kusumota Luiz de Souza ◽  
Adam Muslem George Burgess ◽  
Iain Wyllie Chalmers ◽  
Karl Francis Hoffmann ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Molecular Basis ◽  
Selective Inhibition ◽  
Class Ii

scholarly journals Characterization of mycobacterial protein glycosyltransferase activity using synthetic peptide acceptors in a cell-free assay

Glycobiology ◽  
2002 ◽  
Vol 12 (7) ◽  
pp. 427-434 ◽  
Author(s):  
H. N. Cooper ◽  
S. S. Gurcha ◽  
J. Nigou ◽  
P. J. Brennan ◽  
J. T. Belisle ◽  
...  
Keyword(s):  
Synthetic Peptide ◽  
A Cell ◽  
Mycobacterial Protein

scholarly journals Characterization of human lysosomal neuraminidase defines the molecular basis of the metabolic storage disorder sialidosis.

1996 ◽  
Vol 10 (24) ◽  
pp. 3156-3169 ◽  
Author(s):  
E Bonten ◽  
A van der Spoel ◽  
M Fornerod ◽  
G Grosveld ◽  
A d'Azzo
Keyword(s):  
Molecular Basis ◽  
Storage Disorder

scholarly journals Characterization of the Molecular Basis of Group II Intron RNA Recognition by CRS1-CRM Domains

2008 ◽  
Vol 283 (34) ◽  
pp. 23333-23342 ◽  
Author(s):  
Ido Keren ◽  
Liron Klipcan ◽  
Ayenachew Bezawork-Geleta ◽  
Max Kolton ◽  
Felix Shaya ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Group Ii Intron ◽  
Rna Recognition ◽  
Group Ii

Characterization of Surfactant Subtypes of Beractant and a Synthetic Peptide Containing Surfactant KL4 following Surface Area Cycling and Addition of Fibrinogen

Lung ◽  
1997 ◽  
Vol 175 (4) ◽  
pp. 225-233 ◽  
Author(s):  
E. Manalo ◽  
T. A. Merritt ◽  
J. D. Amirkhanian ◽  
A. Kheiter
Keyword(s):  
Surface Area ◽  
Synthetic Peptide

Polyclonal human rheumatoid factors cross-reacting with histone H3: Characterization of an idiotope on the H3 binding site

1990 ◽  
Vol 10 (4) ◽  
pp. 211-219 ◽  
Author(s):  
Thierry Martin ◽  
Anne Marie Knapp ◽  
Sylvianne Muller ◽  
Jean Louis Pasquali
Keyword(s):  
Binding Site ◽  
Histone H3 ◽  
Rheumatoid Factors
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