scholarly journals Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas.

1981 ◽  
Vol 154 (2) ◽  
pp. 333-346 ◽  
Author(s):  
L E Posner ◽  
M Robert-Guroff ◽  
V S Kalyanaraman ◽  
B J Poiesz ◽  
F W Ruscetti ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
Solid Phase ◽  
Leukemia Virus ◽  
Calf Serum ◽  
Natural Antibodies ◽  
T Cell Lymphoma ◽  
T Cell Lymphomas ◽  
Human T Cell ◽  
Core Proteins

Sera from patients with cutaneous T cell lymphoma and leukemia were screened for the presence of natural antibody to the human T cell lymphoma (leukemia) virus, HTLVCR, using a solid-phase radioimmunoassay. Sera from two patients, including patient CR, from whose cultured T lymphoblastic cell line (HUT102), the retrovirus HTLVCR was isolated, reacted specifically with proteins of HTLVCR. Serum from patient CR also reacted specifically with proteins of HTLVMB, an independent but highly related retroviral isolate from a patient with Sezary T cell leukemia. The specificity for HTLVCR proteins was demonstrated by solid-phase immunocompetition assays and competition radioimmunoprecipitation assays. Analysis of radioimmunoprecipitates indicated that the natural antibodies were directed against HTLVCR core proteins with molecular weights of 24,000 and 19,000 (p24 and p19). Whereas the serum reactivities for HTLVCR proteins were shown to be highly specific, additional reactivities seen against proteins of animal retroviruses including GaLV, SSV, FeLV, and BaEV were clearly shown not to be viral specific but rather were due to reactivity with cellular antigens contaminating the viral preparations or with related antigens present in fetal calf serum. These results demonstrating natural antibodies to HTLVCR provide the first evidence for a specific antibody response to a retrovirus in humans.

scholarly journals Seroprevalence of human T-cell lymphoma/leukemia virus type-1 (HTLV-1) antibodies among blood donors at Enugu, Nigeria

2015 ◽  
pp. 31 ◽  
Author(s):  
Robinson Onoh ◽  
Augustine Okoye ◽  
Godswill Obed Ibegbulam ◽  
Ngozi Immaculata Ugwu ◽  
Chukwudi Siemon Anigbo ◽  
...  
Keyword(s):  
T Cell ◽  
Virus Type ◽  
Blood Donors ◽  
Cell Lymphoma ◽  
Leukemia Virus ◽  
T Cell Lymphoma ◽  
Human T Cell

scholarly journals Detection of the human T cell lymphoma virus p19 in cells of some patients with cutaneous T cell lymphoma and leukemia using a monoclonal antibody.

1981 ◽  
Vol 154 (6) ◽  
pp. 1957-1964 ◽  
Author(s):  
M Robert-Guroff ◽  
F W Ruscetti ◽  
L E Posner ◽  
B J Poiesz ◽  
R C Gallo
Keyword(s):  
Monoclonal Antibody ◽  
T Cells ◽  
T Cell ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Cutaneous T Cell Lymphoma ◽  
Natural Reservoir ◽  
T Cell Lymphomas ◽  
Human T Cell ◽  
Type C

A monoclonal antibody specific for the internal p19 protein of a type-C retrovirus (HTLV) isolated from human neoplastic T cells has been developed. Its specificity has been shown by radioimmune precipitation and by affinity chromatography of iodinated HTLV proteins. By indirect immune fluorescence this antibody recognizes only HTLV-producing cells. Examination of cells from patients with cutaneous T cell lymphomas and leukemias and with other types of lymphomas and leukemias indicated that HTLV p19 expression is rare. The monoclonal antibody will be useful in determining the natural reservoir of HTLV, possibly in a subset of mature T cell neoplasias.

Impact of Growth Factor Independence 1 in Human T-Cell Lymphomas; Pathogenic Potential Identified by Insertional Mutagenesis in a Murine T-Cell Lymphoma Model.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 5047-5047
Author(s):  
Magdalena Julia Dabrowska ◽  
Karen Dybkaer ◽  
Preben Johansen ◽  
Hans Erik Johnsen ◽  
Finn Skou Pedersen
Keyword(s):  
T Cell ◽  
Protein Expression ◽  
Posttranscriptional Regulation ◽  
Immunohistochemical Staining ◽  
Insertional Mutagenesis ◽  
Cell Lymphoma ◽  
Expression Patterns ◽  
T Cell Lymphoma ◽  
T Cell Lymphomas ◽  
Human T Cell

Abstract Abstract 5047 Introduction The transcriptional repressor and oncogene Growth factor independence 1 (Gfi1) has a major oncogenic potential and is aberrantly expressed in murine lymphomas and several human cancers. Gfi1 is a key regulator of stem cell quiescence and plays a significant role in T-cell development, lineage commitment, and influences development of maturate granulocytes and monocytes. The genomic locus on murine chromosome 5 encoding Gfi1 is a frequent integration locus activated in T-cell lymphomas induced by the SL3-3 Murine Leukemia Virus (MLV) as well as other MLVs, indicating that Gfi1 is essential in development of these tumors. In the SL3-3 induced T-cell lymphoma model, retroviral insertions in the Gfi1 3'UTR have been demonstrated to decouple microRNA-mediated posttranscriptional regulation of protein expression (Dabrowska et al, 2009) further supporting its role in lymphomagenesis. In human cancers, Gfi1 protein expression has been observed in HTLV-1 induced ATLL and SCLC but no knowledge exists on how Gfi1 contributes to initiating and maintaining human T-cell lymphomas. Methods Gfi1 gene and protein expression patterns were determined in precursor and mature human T-cell lymphomas by real time PCR and Western blot analysis. Furthermore, localization and expression patterns of the Gfi1 protein was determined in the human T-cell lymphomas by immunohistochemical staining with Gfi1 antibodies and compared to similar staining of MLV induced tumors. Results Our results demonstrated that Gfi1 mRNA and protein levels vary significantly among the human T-cell lymphomas, and do not always show a direct proportional pattern. Thus, Gfi1 mRNA expression can be relatively high without resulting in a corresponding high protein expression, suggesting that a microRNA mediated posttranscriptional regulation exists in some tumors but may be disrupted in others. Furthermore, an additional Gfi1 protein variant was identified in one of the T-cell lymphoma entities. Immunohistochemical staining demonstrated varying Gfi1 protein expression in both nucleus and cytoplasm in the T-cell lymphomas and different distributions of the protein within the tumor and tumor cells were observed among samples. Staining of normal human tonsil demonstrated Gfi1 protein to be localized in the cytoplasm. We hypothesise that regulation of Gfi1 may include shuttling between cytoplasm and nucleus and that lymphomagenesis enables unlimited nuclear access. Conclusion Our data shows that deregulated Gfi1 expression plays a major role in the development of MLV induced lymphomas and strongly indicates that retroviral insertional mutagenesis in murine models of human NHLs can be used to identify new genes involved in lymphomagenesis and, by use of functional assays, their impact on human lymphomas can be evaluated. Disclosures No relevant conflicts of interest to declare.

Antibodies to the Human T-Cell Lymphoma/Leukemia Virus Type I in Dutch Haemophiliacs

Vox Sanguinis ◽  
1986 ◽  
Vol 50 (1) ◽  
pp. 12-15 ◽  
Author(s):  
Jaap Goudsmit ◽  
Frank Miedemca ◽  
Cees Breederveld ◽  
Fokke Terpstra ◽  
Marijke Roos ◽  
...  
Keyword(s):  
T Cell ◽  
Virus Type ◽  
Cell Lymphoma ◽  
Leukemia Virus ◽  
T Cell Lymphoma ◽  
Type I ◽  
Human T Cell
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