scholarly journals Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide.

1979 ◽  
Vol 149 (3) ◽  
pp. 669-685 ◽  
Author(s):  
F Orskov ◽  
I Orskov ◽  
A Sutton ◽  
R Schneerson ◽  
W Lin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Sialic Acid ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Chemical Basis ◽  
13C Nuclear Magnetic Resonance ◽  
Serologic Method ◽  
Escherichia Coli K1 ◽  
Immunologic Properties

The chemical basis for the alternating antigenic change called form variation noted for the Escherichia coli K1-capsular polysaccharide has been shown by 13C nuclear magnetic resonance to be a result of random O-acetylation of C7 and C9 carbons of the alpha-2-8-linked sialic acid homopolymer. A serologic method (antiserum agar) was developed to identify and isolate the form variants. The O-acetyl positive and O-acetyl negative K1 polysaccharides had unique biochemical and immunologic properties. The O-acetyl-positive variants resisted neuraminidase hydrolysis in contrast to the susceptibility of the O-acetyl negative variant to this enzyme. In addition, O-acetylation altered the antigenicity of the O-acetyl polysaccharides. When injected as whole organisms, O-acetyl positive organisms produced anti-K1 -antibodies in rabbits specific for this polysaccharide variant. O-acetyl negative organisms were comparatively less immunogenic; however, antibodies induced by these organisms reacted with both K1 polysaccharide variants. Burros, injected with either variant, produced antibodies reactive with both K1 polysaccharides.

Structural elucidation of the capsular polysaccharide antigen of Neisseria meningitidis serogroup Z using 13C nuclear magnetic resonance

1979 ◽  
Vol 57 (22) ◽  
pp. 2902-2907 ◽  
Author(s):  
Harold J. Jennings ◽  
Karl-Gunnar Rosell ◽  
C. Paul Kenny
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Neisseria Meningitidis ◽  
Capsular Polysaccharide ◽  
Resonance Spectrum ◽  
Coupling Constants ◽  
Polysaccharide Antigen ◽  
Phosphate Diester ◽  
13C Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

The capsular polysaccharide antigen from Neisseriameningitidis serogroup Z contains equimolar quantities of 2-acetamido-2-deoxy-D-galactose, glycerol, and phosphate. Carbon-13 nuclear magnetic resonance indicates that the polysaccharide is composed by a repeating unit of 1′-O-2-acetamido-2-deoxy-α-D-galactopyranosyl-glycerol joined through phosphate diester groups at O-3′ of glycerol and O-3 of the galactosamine residue. Assignments of the signals in the carbon-13 nuclear magnetic resonance spectrum were made by consideration of the previously assigned signals of related monomers and oligomers. When applied to a Karplus type relationship the values of the 3J(13C–31P) coupling constants were consistent with the polysaccharide having an extended conformation.

Use of a bacteriophage to depolymerize a polysaccharide to an oligosaccharide; comparison of the 1H and 13C nuclear magnetic resonance spectra of the polymer and its hexasaccharide repeating unit

1980 ◽  
Vol 58 (23) ◽  
pp. 2588-2591 ◽  
Author(s):  
Guy G. S. Dutton ◽  
Angela V. Savage ◽  
Michel Vignon
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nmr Spectra ◽  
Capsular Polysaccharide ◽  
Nuclear Magnetic Resonance Spectra ◽  
13C Nuclear Magnetic Resonance ◽  
Magnetic Resonance Spectra ◽  
Nuclear Magnetic

A β-D-galactosidase associated with bacteriophage [Formula: see text] has been used to depolymerize the capsular polysaccharide of Klebsiella serotype K18 into the hexasaccharide corresponding to one repeating unit of the polymer. The nmr spectra of the polymer and of the oligosaccharide are comparable, leading to the conclusion that the conformations in solution of the repeating unit of the two substances are similar.

Putrescine distribution in Escherichia coli studied in vivo by 13C nuclear magnetic resonance

1984 ◽  
Vol 805 (4) ◽  
pp. 337-344 ◽  
Author(s):  
Benjamín Frydman ◽  
Rosalía B. Frydman ◽  
Carlos de Los Santos ◽  
Daniel Alonso Garrido ◽  
Sara H. Goldemberg ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
13C Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

scholarly journals A Revised Structure for the Glycolipid Terminus of Escherichia coli K5 Heparosan Capsular Polysaccharide

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1516
Author(s):  
Lufeng Yan ◽  
Li Fu ◽  
Ke Xia ◽  
Shiguo Chen ◽  
Fuming Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Enzymatic Digestion ◽  
Nuclear Magnetic

The structure of heparosan capsular polysaccharide (CPS) has been determined using enzymatic digestion with nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Previous errors in the assignment of the glycolipid acceptor structure, from which heparosan is extended, have been corrected. The structure of heparosan CPS is GlcNAc α-1,[4GlcA β-1,4GlcNAc α-1,]n4GlcA β-1,[4Kdo β-2,7Kdo β-2,]0 or 14Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-PG-I (C16:0 or C18:0) (where n is ~250 for a CPS of 100 kDa).

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