scholarly journals A Revised Structure for the Glycolipid Terminus of Escherichia coli K5 Heparosan Capsular Polysaccharide

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1516
Author(s):  
Lufeng Yan ◽  
Li Fu ◽  
Ke Xia ◽  
Shiguo Chen ◽  
Fuming Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Capsular Polysaccharide ◽  
Enzymatic Digestion ◽  
Nuclear Magnetic

The structure of heparosan capsular polysaccharide (CPS) has been determined using enzymatic digestion with nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Previous errors in the assignment of the glycolipid acceptor structure, from which heparosan is extended, have been corrected. The structure of heparosan CPS is GlcNAc α-1,[4GlcA β-1,4GlcNAc α-1,]n4GlcA β-1,[4Kdo β-2,7Kdo β-2,]0 or 14Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-2,7Kdo β-2,4Kdo β-PG-I (C16:0 or C18:0) (where n is ~250 for a CPS of 100 kDa).

scholarly journals Special Issue: NMR-Based Metabolomics

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3283
Author(s):  
Miriam Pérez-Trujillo ◽  
Toby J. Athersuch
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Dynamic Range ◽  
Chemical Information ◽  
Special Issue ◽  
The Core ◽  
Analytical Platforms ◽  
Nuclear Magnetic

Nuclear magnetic resonance (NMR) spectroscopy remains one of the core analytical platforms for metabolomics, providing complementary chemical information to others, such as mass spectrometry, and offering particular advantages in some areas of research on account of its inherent robustness, reproducibility, and phenomenal dynamic range [...]

scholarly journals β-D-galactopyranoside Seco-phytoporphyrin from Atropa Belladonna and Solanum Tuberosum Yellow Leaves Determined by Nuclear Magnetic Resonance

2021 ◽  
Author(s):  
Nina Djapic
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Solanum Tuberosum ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Column Chromatography ◽  
Atropa Belladonna ◽  
Structure Confirmation ◽  
Chlorophyll Catabolite ◽  
Nuclear Magnetic

The study described the isolation of β-D-galactopyranoside seco-phytoporphyrin from Atropa belladonna and Solanum tuberosum yellow leaves. Column chromatography was applied for its isolation. The UV-Vis, mass spectrometry and NMR spectroscopy were used for the structure confirmation. The results demonstrate that the two plants that the same chlorophyll catabolite structure.

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