scholarly journals THE CELL WALLS OF GROUP D STREPTOCOCCI

1965 ◽  
Vol 122 (2) ◽  
pp. 237-249 ◽  
Author(s):  
Arnold S. Bleiweis ◽  
Richard M. Krause
Keyword(s):  
Cell Walls ◽  
Antigenic Determinant ◽  
New Technique ◽  
Glycosidic Bond ◽  
Terminal Residue ◽  
A New Technique ◽  
Inhibition Studies ◽  
Group D

Group D Types 1 and 26 cell walls and the corresponding type-specific carbohydrates, extracted from the walls by various means, contain rhamnose, glucose, galactose, N-acetylglucosamine, and N-acetylgalactosamine. Mucopeptide elements are also present in the walls and in enzymatically-extracted carbohydrates. Types 1 and 26 carbohydrates extracted by formamide contain no mucopeptide, but the serologic reactivity of Type 1 antigen is destroyed by this procedure. The Type 1 antigen was successfully extracted, however, by a new technique involving autolysis of cell walls at pH 6.2. The type carbohydrate prepared by this procedure has chemical and serological similarities to the antigens prepared by the S. albus enzyme and the lysozyme methods. Quantitative precipitin inhibition studies with Type 1 antigen and antibody indicate that D-glucose and N-acetylglucosamine may be components of the antigenic determinant. The terminal residue is probably bound by an ß-glycosidic bond to the subterminal sugar. Similar studies with the Type 26 carbohydrate have not revealed any of the chemical features of the antigenic determinant.

Sphenoid dysplasia in neurofibromatosis type 1: a new technique for repair

2017 ◽  
Vol 33 (6) ◽  
pp. 983-986 ◽  
Author(s):  
Di Rocco Concezio ◽  
Samii Amir ◽  
Tamburrini Gianpiero ◽  
Massimi Luca ◽  
Giordano Mario
Keyword(s):  
Neurofibromatosis Type 1 ◽  
Neurofibromatosis Type ◽  
New Technique ◽  
Sphenoid Dysplasia ◽  
A New Technique

Cell Walls of Group D Streptococci II. Chemical Studies on the Type 1 Antigen Purified from the Autolytic Digest of Cell Walls

1967 ◽  
Vol 94 (5) ◽  
pp. 1381-1387 ◽  
Author(s):  
Arnold S. Bleiweis ◽  
Frank E. Young ◽  
Richard M. Krause
Keyword(s):  
Cell Walls ◽  
Chemical Studies ◽  
Group D

scholarly journals Correction to: Sphenoid dysplasia in neurofibromatosis type 1: a new technique for repair

2017 ◽  
Vol 33 (12) ◽  
pp. 2211-2211 ◽  
Author(s):  
Concezio Di Rocco ◽  
Amir Samii ◽  
Gianpiero Tamburrini ◽  
Luca Massimi ◽  
Mario Giordano
Keyword(s):  
Neurofibromatosis Type 1 ◽  
Neurofibromatosis Type ◽  
New Technique ◽  
Sphenoid Dysplasia ◽  
A New Technique

scholarly journals STUDIES ON THE IMMUNOCHEMISTRY OF STREPTOCOCCAL MUCOPEPTIDE

1966 ◽  
Vol 124 (2) ◽  
pp. 155-171 ◽  
Author(s):  
Walter W. Karakawa ◽  
Richard M. Krause
Keyword(s):  
Cell Walls ◽  
Antigenic Determinant ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Amino Acid Residues ◽  
Precipitin Reaction ◽  
Peptide Moiety ◽  
Streptomyces Albus ◽  
D Cell ◽  
Group D

Streptococcal mucopeptide, solubilized by either ultrasonic treatment or lysozyme, gave a precipitin reaction with rabbit antimucopeptide serum. A haptenic inhibitor of this reaction, which was composed of alanine, glutamic acid, and lysine in a mole ratio of 4:1:1, was isolated from a Streptomyces albus enzymes digest of Group D cell walls by ion exchange chromatography. When selected antisera were employed, greater than 90% inhibition of the mucopeptide quantitative precipitin reaction was achieved with 2 mg/ml of this inhibitor, whereas a hexosamine fraction with minimal concentrations of amino acid residues was inactive in this respect. These results suggest that the peptide moiety is an antigenic determinant of mucopeptide. Preliminary results indicate that the hexosamine polymer of the mucopeptide is a secondary antigenic determinant.

scholarly journals ANTIGENIC RELATIONSHIPS BETWEEN GROUPS B AND G STREPTOCOCCI

1964 ◽  
Vol 120 (4) ◽  
pp. 629-637 ◽  
Author(s):  
Stephen N. Curtis ◽  
Richard M. Krause
Keyword(s):  
Cell Walls ◽  
Antigenic Determinant ◽  
Cross Reactivity ◽  
Significant Component ◽  
The Cross ◽  
Group B ◽  
Inhibition Studies ◽  
Antigenic Relationships

Trypsinized cell walls of Group B hemolytic streptococci are composed of a group-specific carbohydrate and mucopeptide. The carbohydrate extracted with hot formamide is composed of rhamnose, N-acetylglucosamine, and galactose. Quantitative precipitin inhibition studies have shown that L rhamose is the significant component of the antigenic determinant. The cross-reactivity between B and G carbohydrates is dependent upon the fact that Lrhamose is a determinant sugar in both antigens.

A technique to study the development in wood of soft rot fungi and its application to Ceratocystis ulmi

1971 ◽  
Vol 49 (1) ◽  
pp. 155-159 ◽  
Author(s):  
F. Casagrande ◽  
G.B. Ouellette
Keyword(s):  
Cell Walls ◽  
Close Contact ◽  
Soft Rot ◽  
Senior Author ◽  
New Technique ◽  
Starch Grains ◽  
A New Technique ◽  
New System

The development of fungi causing soft rots and of ensuing cavities in cell walls was studied with a new technique, consisting in inoculation of sterilized wood sections placed on agar slants. The senior author proposes a new system of classification of the soft rot fungi. Applied to investigations on Ceratocystis ulmi (Buism.) C. Moreau, this technique permits further characterization of the morphology of this fungus, including the formation of microhyphae and microspore-like particles, often less than 0.5 μ in diameter, and recognition of the various configurations brought about by its growth in wood tissues. For example, microhyphae grow in a reticulate manner in close contact with starch grains, and these seem to break down.

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