scholarly journals IMMUNOCYTOCHEMICAL STUDY OF GAMMA GLOBULIN IN LIVER IN HEPATITIS AND POSTNECROTIC CIRRHOSIS

1960 ◽  
Vol 111 (2) ◽  
pp. 285-294 ◽  
Author(s):  
Seymour Cohen ◽  
Goroku Ohta ◽  
Edward J. Singer ◽  
Hans Popper
Keyword(s):  
Lymph Nodes ◽  
Liver Cell ◽  
Liver Diseases ◽  
Gamma Globulin ◽  
Plasma Cells ◽  
Fluorescence Technique ◽  
Immunocytochemical Study ◽  
Postnecrotic Cirrhosis ◽  
Spleen And Lymph Nodes ◽  
Cell Breakdown

Gamma globulin was demonstrated by immunocytochemical fluorescence technique in many reticuloendothelial cells of the hepatic sinusoids and of the fibrous tracts in various forms of hepatitis and in postnecrotic cirrhosis. In other liver diseases and in normal livers, even in the presence of hypergammaglobulinemia, few if any gamma globulin-containing cells were found. In contrast, spleen and lymph nodes showed no difference between postnecrotic cirrhosis or hepatitis and other types of cirrhosis or non-hepatic hypergammaglobulinemias. The gamma globulin-containing cells in the liver are on cytologic grounds considered reticuloendothelial cells showing transition to plasma cells and exhibiting little or no phagocytosis of tissue breakdown products. These cells are assumed to form rather than engulf gamma globulin. The possibility that the gamma globulin formed represents antibody to liver cell breakdown products is discussed.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF LYMPHATIC TISSUE IN RUNT DISEASE

1965 ◽  
Vol 25 (3) ◽  
pp. 149-177 ◽  
Author(s):  
Leon Weiss ◽  
Alan C. Aisenberg
Keyword(s):  
Lymph Nodes ◽  
Plasma Cells ◽  
Cell Types ◽  
Sprague Dawley ◽  
Connective Tissues ◽  
Splenic Cells ◽  
Reticular Cells ◽  
Spleen And Lymph Nodes ◽  
Myelin Figure ◽  
Rats And Mice

The thymus, spleen, and lymph nodes were studied in runt disease induced by a graft of intravenously injected homologous splenic cells into newborn rats and mice. Adult Long-Evans cells (70 x 106) were injected into Sprague-Dawley rats. Adult DBA cells (7 x 106) were injected into C57BL/6 mice. Runted rats were sacrificed at 14 to 28 days of age; mice at 10 to 20 days. The thymic cortex is depleted of small lymphocytes. Those remaining are severely damaged and phagocytized. Evidence of damage includes swelling of mitochondria, myelin figure formation, margination of chromatin, and sharp angulation in nuclear contour. Large numbers of macrophages are present. Epithelial-reticular cells which envelop small cortical blood vessels are often retracted, with the result that the most peripheral layer in the thymic-blood barrier suffers abnormally large gaps. Lymphocytes of the periarterial lymphatic sheaths of spleen and of the cortex of lymph nodes are reduced in number and damaged. Vast numbers of plasma cells and many lymphocytes are evident throughout lymph nodes, in the periarterial lymphatic sheaths, and in the marginal zone and red pulp of the spleen. Plasma cells are of different sizes, the larger having dilated sacs of endoplasmic reticulum. Lymphocytes are small to medium in size. They contain, in varying quantity, ribosomes and smooth membrane-bounded cytoplasmic vesicles approximately 350 to 500 A in diameter. Most plasma cells and lymphocytes are damaged and many of these are phagocytized. Many lymphocytes in lymph nodes, however, show no evidence of damage. Reticular cells and other fixed cells of the connective tissues seldom appear affected. Thus, the major cell types reacting in runt disease are lymphocytes, plasma cells, and histiocytes or macrophages. It appears, therefore, that both the delayed and immediate types of sensitivity play a part in this disease.

DEFICIENCY OF IgM

PEDIATRICS ◽  
1971 ◽  
Vol 47 (2) ◽  
pp. 399-404
Author(s):  
W. P. Faulk ◽  
W. S. Kiyasu ◽  
M. D. Cooper ◽  
H. H. Fudenberg
Keyword(s):  
Lymph Nodes ◽  
Plasma Cells ◽  
Germinal Centers ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Pseudomonas Infection ◽  
Spleen And Lymph Nodes ◽  
Genetically Determined

An 8½-month-old infant with absent IgM had recurrent Pseudomonas infections. IgG and IgA, but no IgM-containing plasma cells, were identified in the spleen by immunofluorescence. The spleen and lymph nodes lacked germinal centers, but Peyer's patches and the appendix were normal. The absence of IgM was perhaps genetically determined because the father's serum IgM was also low. This may have predisposed to the Pseudomonas infection, since antibodies to Pseudomonas are predominantly IgM.

PRESENCE OF PLASMA CELLS AND γ1M-GLOBULIN SYNTHESIS IN A PATIENT WITH THYMIC ALYMPHOPLASIA

PEDIATRICS ◽  
1966 ◽  
Vol 37 (3) ◽  
pp. 485-492
Author(s):  
Philip Fireman ◽  
Horton A. Johnson ◽  
David Gitlin
Keyword(s):  
Bone Marrow ◽  
Lymph Nodes ◽  
Plasma Cells ◽  
Peripheral Circulation ◽  
Serum Concentrations ◽  
Normal Numbers ◽  
Bone Marrow Plasma ◽  
Severe Infections ◽  
Spleen And Lymph Nodes

A child is described who had thymic alymphoplasia with increased serum concentrations of γ1M-globulins and a deficiency of γ1A- and 7S γ2-globulins; the child was subject to severe infections from 2 weeks of age and succumbed to pneumonia at 11 months of age. Even though the tissues of this patient at necropsy revealed a remarkable paucity of lymphocytes, there were relatively normal numbers of lymphocytes in his peripheral circulation and his bone marrow. Plasma cells were readily demonstrated in the spleen and lymph nodes. The studies suggest that the development of plasma cells and γ1M-globulin synthesis may occur independently of the development of the thymus and the small lymphocytes in man.

scholarly journals AN ULTRASTRUCTURAL STUDY OF LYMPHOCYTES WITH SURFACE-BOUND IMMUNOGLOBULIN

1972 ◽  
Vol 135 (2) ◽  
pp. 267-276 ◽  
Author(s):  
William D. Perkins ◽  
Morris J. Karnovsky ◽  
Emil R. Unanue
Keyword(s):  
Cell Surface ◽  
Lymph Nodes ◽  
Ultrastructural Study ◽  
Plasma Cells ◽  
Morphological Characteristics ◽  
Cytoplasmic Organelles ◽  
Ultrastructural Radioautography ◽  
Morphological Differences ◽  
Spleen And Lymph Nodes ◽  
Labeling Pattern

This report is on a radioautographic study of lymphocytes exposed to 125I-labeled anti-Ig in an attempt to identify surface-bound Ig molecules. The results as studied by ultrastructural radioautography confirmed the presence of surface-bound Ig on a certain population of lymphocytes. The specificity of the anti-Ig was determined by using appropriate controls that included the use of an absorbed anti-Ig and anti-hemocyanin antibody. The labeling pattern resulting from the interaction of labeled anti-Ig and Ig was found to be specifically associated with the cell surface and random in its distribution. Morphological differences were not apparent between labeled and nonlabeled lymphocytes in the spleen and lymph nodes. In the thymus, most lymphocytes did not exhibit detectable Ig. The few thymic lymphocytes that were labeled had unique morphological characteristics that included fewer ribosomes, many of which were monoribosomes. Relative to the amount in their cytoplasmic organelles, plasma cells had surface Ig but to a lesser degree than lymphocytes. Finally, macrophages were nonspecifically labeled and contained antibody on their membranes as well as intracellularly.

scholarly journals Relapse of Multiple Myeloma Presenting as Extramedullary Plasmacytomas in Multiple Organs

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Murat Köse ◽  
Ersida Buraniqi ◽  
Timur Selçuk Akpinar ◽  
Seyit Mehmet Kayacan ◽  
Tufan Tükek
Keyword(s):  
Multiple Myeloma ◽  
Lymph Nodes ◽  
Adrenal Glands ◽  
Plasma Cells ◽  
Multiple Organ ◽  
Bone Lesions ◽  
Multiple Organs ◽  
First Remission ◽  
Spleen And Lymph Nodes ◽  
Extramedullary Plasmacytomas

Multiple myeloma is a neoplastic plasma cell disorder. It is characterized by collections of abnormal plasma cells accumulating in the bone marrow, where they interfere with the production of normal blood cells. It usually presents as a multisystemic involvement, whose symptoms and signs vary greatly. Some patients have slowly progressive disease while others have aggressive clinical behavior by extramedullary involvement. In addition to renal failure, anemia, hypercalcemia, lytic bone lesions, and immunodeficiency, it also affects multiple organ system, such as pancreas, adrenal glands, kidney, skin, lung, liver, spleen, lymph nodes, and bone. To raise awareness of the variable presentations of this disease, we report a 53-year-old male patient, with multiple myeloma in his first remission who relapsed with extramedullary plasmacytomas (EMPs) involving multiple organs, such as pancreas, adrenal glands, kidney, skin, lung, liver, spleen, and lymph nodes.

scholarly journals CYTOCHEMICAL DETECTION OF SITES OF ANTIBODY TO HORSERADISH PEROXIDASE IN SPLEEN AND LYMPH NODES

1968 ◽  
Vol 16 (4) ◽  
pp. 237-248 ◽  
Author(s):  
WERNER STRAUS
Keyword(s):  
Horseradish Peroxidase ◽  
Lymph Nodes ◽  
Plasma Cells ◽  
Specific Binding ◽  
Repeated Injection ◽  
Similar Reaction ◽  
Fixed Tissue ◽  
Tissue Sections ◽  
Spleen And Lymph Nodes

After repeated injection of small amounts of horseradish peroxidase into the perivertebral muscle and footpads of rabbits, certain sites in spleen and popliteal lymph nodes showed intense adsorption of antigen (horseradish peroxidase) during treatment of fixed tissue sections in vitro. The reaction occurred in certain cells, probably plasma cells, and was also positive in the reticulum extending between lymphocytes. These sites were considered to contain antibodies against horseradish peroxidase, since sections from the same block, not treated with antigen in vitro, and corresponding sections from nonimmunized rabbits did not give a similar reaction. The blood sera of rabbits which showed specific binding of antigen in the spleen caused approximately 60% inhibition of horseradish peroxidase activity, but no inhibition was caused by the blood sera of nonimmunized control animals.

Experimental Trypanosoma brucei Infection in Deer Mice: Splenic Changes

1980 ◽  
Vol 17 (2) ◽  
pp. 218-225 ◽  
Author(s):  
J. E. Moulton
Keyword(s):  
Nervous System ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Trypanosoma Brucei ◽  
Peromyscus Maniculatus ◽  
Plasma Cells ◽  
Germinal Centers ◽  
Deer Mice ◽  
Spleen And Lymph Nodes ◽  
Red Pulp

Forty deer mice (Peromyscus maniculatus) were infected with Trypanosoma brucei organisms and were killed 33 to 83 days after inoculation (average, 63). The outstanding lesion was infiltration of plasma cells in various tissues. These cells caused disruption of the periarteriolar lymphocytic sheaths and thickening of red pulp cords in the spleen. The lymph node was almost completely replaced by plasma cells. The spleen and lymph nodes also had marked hyperplasia of germinal centers and granulomatous-like proliferation of macrophages. The nervous system showed meningoencephalitis characterized by accumulations of plasma cells.

scholarly journals STUDIES ON ANTIBODY PRODUCTION

1955 ◽  
Vol 102 (1) ◽  
pp. 49-60 ◽  
Author(s):  
Albert H. Coons ◽  
Elizabeth H. Leduc ◽  
Jeanne M. Connolly
Keyword(s):  
Lymph Nodes ◽  
Plasma Cells ◽  
Histochemical Demonstration ◽  
Green Fluorescence ◽  
Spleen And Lymph Nodes ◽  
Specific Reactions ◽  
A Minor ◽  
Hyperimmune Rabbit ◽  
In Cells

A method for the specific histochemical demonstration of antibody in cells and parts of cells is described. It consists of carrying out a two stage immunological reaction on frozen sections of tissues: (a) allowing reaction between antibody in the tissue and dilute antigen applied in vitro, and (b) the detection of those areas where this antigen has been specifically absorbed by means of a precipitin reaction carried out with fluorescein-labelled antibody. Examination under the fluorescence microscope reveals the yellow-green fluorescence of fluorescein over those areas where a precipitate has formed. A study of the hyperimmune rabbit on the first few days after the last of a series of intravenous antigen injections reveals that antibody against human γ-globulin or ovalbumin is present in groups of plasma cells in the red pulp of the spleen, the medullary areas of lymph nodes, the submucosa of the ileum, and the portal connective tissue of the liver. Because of extensive non-specific reactions, the bone marrow could not be examined. Small amounts of antibody were occasionally visible in cells in the lymphoid follicles of the spleen and lymph nodes, so that a minor contribution by lymphocytes to antibody synthesis cannot be excluded.

scholarly journals ROLE OF THE THYMUS IN IMMUNE REACTIONS IN RATS

1962 ◽  
Vol 116 (2) ◽  
pp. 187-206 ◽  
Author(s):  
Byron H. Waksman ◽  
Barry G. Arnason ◽  
Branislav D. Janković
Keyword(s):  
Lymph Nodes ◽  
Plasma Cells ◽  
Germinal Centers ◽  
White Pulp ◽  
Size Number ◽  
Splenic White Pulp ◽  
Spleen And Lymph Nodes ◽  
Lymphoid Nodules ◽  
Lymphatic Organs

In rats thymectomized at birth, there was a profound depletion of small lymphocytes in various lymphatic organs. In the spleen, these cells were completely lacking from the Malpighian bodies and splenic white pulp. Empty reticular structures remained surrounding the white pulp arterioles. In the lymph nodes, large masses and nodules of small lymphocytes (primary lymphoid nodules) were either markedly depleted or absent, as were the zones of these cells normally surrounding germinal centers. In both spleen and nodes, germinal centers appeared normal in size, number, and cellular make-up; and plasma cells were found in normal or even increased number in their customary position. Rats which in spite of thymectomy developed intense Arthus or delayed reactivity showed incomplete depletion of the lymphoid tissue. It is concluded that small lymphocytes of the spleen and lymph nodes may come, in large part, directly from the thymus and are not derived from medium and large lymphocytes of the germinal centers. It is suggested that there may be a second population of small lymphocytes whose function is unrelated to the thymus lymphocytes.

Sign in / Sign up

Export Citation Format

Share Document