scholarly journals Proliferation of mitochondria during the cell cycle of the human cell line (HL-60).

1981 ◽  
Vol 89 (2) ◽  
pp. 256-260 ◽  
Author(s):  
T W James ◽  
R Bohman
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Growth Rate ◽  
Dna Synthesis ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Time Base ◽  
Mitochondrial Mass ◽  
Common Time

Using rhodamine 123 to stain mitochondria of the human cell line HL-60, we have followed their increase over the cell cycle by flow cytometry. A near-linear synthesis of mitochondrial mass was shown to occur over the cell cycle. A comparison with the cell's DNA synthesis pattern obtained by the same technique established a common time-base. The mitochondrial synthesis curve changes with culture age. As a control, thd dye was tested for its binding specificity and for its use to resolve mitochondria microscopically. Its stoichiometric range was established and, above 0.25 microgram/ml, it was shown to reduce growth rate and cell viability in culture.

Effects of potassium dichromate on the cell cycle of an established human cell line (NHIK 3025)

Toxicology ◽  
1982 ◽  
Vol 24 (3-4) ◽  
pp. 281-292 ◽  
Author(s):  
Karin Jakobsen ◽  
Oddmund Bakke ◽  
Kjetill Østgaard ◽  
Linda R. White ◽  
Kristen B. Eik-nes
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Human Cell ◽  
Potassium Dichromate ◽  
Human Cell Line

scholarly journals Cell cycle analysis in a human cell line (EUE cells)

Cytometry ◽  
2005 ◽  
Vol 2 (6) ◽  
pp. 426-430 ◽  
Author(s):  
R. Zippel ◽  
E. Martegani ◽  
M. Vanoni ◽  
G. Mazzini ◽  
L. Alberghina
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Cell Cycle Analysis

Steroid responsiveness of the human cell line NHIK 3025

1981 ◽  
Vol 97 (4) ◽  
pp. 551-558
Author(s):  
Kjetill Østgaard ◽  
Einar Wibe ◽  
Kristen B. Eik-Nes
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Androgen Receptor ◽  
Cell Growth ◽  
Cell Line ◽  
Human Cell ◽  
Calf Serum ◽  
Human Cell Line ◽  
Foetal Calf Serum ◽  
Steroid Responsiveness

Abstract. The human cell line NHIK 3025, derived from a carcinoma of the uterine cervix, contains a glucocorticoid and an androgen receptor. The effect of various natural and synthetic steroid hormones and antihormones on growth rate of these cells was therefore investigated. Cells grown in Eagle's MEM with 10% foetal calf serum exhibited reduced growth when cultured with dexamethasone due to prolongation of the cell cycle. Glucocorticoid anti-inducers like progesterone had no significant effect on cell growth. Methyltrienolone (R 1881) or 5α-dihydrotestosterone did not affect cell proliferation. The reported shortening of the cell cycle by testosterone is probably not directly connected with activation of the androgen receptor present, but possibly dependent on metabolic conversion of testosterone to the more potent growth stimulator 4-androstene-3β, 17β-diol. The effect of several anti-androgens was also studied. The non-steroidal anti-androgens flutamide and SCH 16483 had no significant effect on cell proliferation. It was, however, found that a number of steroid antiandrogens, including R2956, stimulated cell growth. A significant stimulatory effect by R2956 was seen within the first cell generation, 4-androstene-3β,17β-diol had to be present during 2 days, and testosterone for even longer times before a similar effect on cell growth could be obtained.

scholarly journals Efficient and crucial quality control of HAP1 cell ploidy status

Biology Open ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. bio057174 ◽  
Author(s):  
Tobias B. Beigl ◽  
Ine Kjosås ◽  
Emilie Seljeseth ◽  
Nina Glomnes ◽  
Henriette Aksnes
Keyword(s):  
Flow Cytometry ◽  
Quality Control ◽  
Cell Line ◽  
Cell Lines ◽  
Human Cell ◽  
Cell Sorting ◽  
Gene Editing ◽  
Human Cell Line ◽  
Popular Subject ◽  
Ploidy Status

ABSTRACTThe near-haploid human cell line HAP1 recently became a popular subject for CRISPR/Cas9 editing, since only one allele requires modification. Through the gene-editing service at Horizon Discovery, there are at present more than 7500 edited cell lines available and the number continuously increases. The haploid nature of HAP1 is unstable as cultures become diploid with time. Here, we demonstrated some fundamental differences between haploid and diploid HAP1 cells, hence underlining the need for taking control over ploidy status in HAP1 cultures prior to phenotyping. Consequently, we optimized a procedure to determine the ploidy of HAP1 by flow cytometry in order to obtain diploid cultures and avoid ploidy status as an interfering variable in experiments. Furthermore, in order to facilitate this quality control, we validated a size-based cell sorting procedure to obtain the diploid culture more rapidly. Hence, we provide here two streamlined protocols for quality controlling the ploidy of HAP1 cells and document their validity and necessity.This article has an associated First Person interview with the co-first authors of the paper.

scholarly journals Estrogen Receptor in a Human Cell Line (MCF-7) from Breast Carcinoma

1973 ◽  
Vol 248 (17) ◽  
pp. 6251-6253 ◽  
Author(s):  
Samuel C. Brooks ◽  
Elizabeth R. Locke ◽  
Herbert D. Soule
Keyword(s):  
Estrogen Receptor ◽  
Breast Carcinoma ◽  
Cell Line ◽  
Human Cell ◽  
Human Cell Line ◽  
Mcf 7

Local Regulation of Adrenal Steroidogenesis: Subtle in vitro Effects of IL-1β on the Human Cell Line NCI-H295R Steroid Production along with Changes in MicroRNA Profile and Orphan Nuclear Receptors NR4As

2021 ◽  
pp. 1-11
Author(s):  
Natalia Santucci ◽  
Rocío Stampone ◽  
Eduardo Brandão Ferreira da Silva ◽  
Silvina Villar ◽  
Silvana Spinelli ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Cell Line ◽  
Nuclear Receptors ◽  
Human Cell ◽  
Human Cell Line ◽  
Mirna Profile ◽  
Orphan Nuclear Receptors ◽  
Steroid Production ◽  
Adrenal Steroid ◽  
Adrenal Steroidogenesis

<b><i>Introduction:</i></b> IL-1β, a cytokine from the innate immune response, is well known for its proinflammatory effects and stimulating activity on the hypothalamus-pituitary-adrenal axis, leading to the pituitary synthesis of adrenocorticotropic hormone followed by cortisol (and dehydroepiandrosterone – DHEA) release by the adrenal gland. While IL-1β modulates the adrenal steroidogenesis at the central level, it is unclear whether it also exerts an effect on the adrenal gland. <b><i>Method:</i></b> We studied the effect of IL-1β on adrenal steroid production and steroidogenic enzyme RNA expression in the human cell line NCI-H295R. We also explored eventual changes in the microRNA (miRNA) profile from IL-1β-treated NCI-H295R cells. <b><i>Results:</i></b> Transcripts encoding IL-1β receptors 1 and 2 were noticeable in the cell line, with cortisol and DHEA production showing a subtle increase after cytokine treatment. Transcripts from key enzymes in the steroidogenic pathway were analyzed, with no noticeable changes on them. The miRNA profile was modified by IL-1β treatment to an extent which bears some relationship with the regulatory mechanisms underlying adrenal steroid production. Since orphan nuclear receptors NR4As have emerged as potential key factors for coordinating inflammatory and metabolic responses, cell expression studies were also carried out to show an NR4As transcript augmentation following IL-1β treatment. <b><i>Discussion/Conclusions:</i></b> The subtle increase in adrenal steroid production in response to IL-1β stimulation without any modification in the transcription of the steroidogenic enzymes analyzed suggests an additional inflammatory/anti-inflammatory loop, wherein NR4As receptors may participate. Besides its physiological role, this process might be implied in pathological states accompanied by an unbalanced immune-endocrine relationship.

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