scholarly journals A sensitive method for measuring polymerized and depolymerized forms of tubulin in tissues.

1977 ◽  
Vol 74 (2) ◽  
pp. 341-350 ◽  
Author(s):  
D G Pipeleers ◽  
M A Pipeleers-Marichal ◽  
P Sherline ◽  
D M Kipnis
Keyword(s):  
Electron Microscopy ◽  
Rat Brain ◽  
Rapid Method ◽  
Binding Assay ◽  
Fed State ◽  
Stabilizing Solution ◽  
Rat Liver Cells ◽  
Wet Weight ◽  
Brain Tubulin ◽  
Sensitive Method

A rapid method for measuring polymerized and depolymerized forms of tubulin in tissues has been developed. The procedure consists of homogenization and centrifugation of the tissue in a microtubule-stabilizing solution and depolymerization of the precipitated microtubules; polymerized and depolymerized forms of tubulin are quantitated by a colchicine-binding assay. The validity of the technique was assessed by electron microscopy and recovery studies with labeled and unlabeled preparations of polymerized and depolymerized forms of rat brain tubulin. The sensitivity of this technique allows quantitation of tubulin in 150 micrograms of tissue, wet weight. The method demonstrated that both the polymerized and depolymerized forms of tubulin were present in rat liver cells, and that in the fed state 31.3 +/-0.7% of the total tubulin pool was in the polymerized form.

scholarly journals A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain

1978 ◽  
Vol 176 (2) ◽  
pp. 365-370 ◽  
Author(s):  
R F G Booth ◽  
J B Clark
Keyword(s):  
Electron Microscopy ◽  
Rat Brain ◽  
Rapid Method ◽  
Sucrose Gradient ◽  
Enzyme Assays ◽  
Marker Enzyme ◽  
Flotation Technique

A rapid (less than 2h) method is described for the preparation of synaptosomes from rat brain by using a discontinuous Ficoll/sucrose gradient by a flotation technique. These synaptosomes are metabolically active and minimally (less than 5%) contaminated with ‘free’ mitochondria as judged by marker-enzyme assays and electron microscopy.

Maytansine-Induced Mitotic Arrest in Human Lymphoblasts

1977 ◽  
Vol 35 ◽  
pp. 460-461
Author(s):  
Tai-Te Chao ◽  
John Sullivan ◽  
Awtar Krishan
Keyword(s):  
Electron Microscopy ◽  
Cell Cycle ◽  
Transmission Electron Microscopy ◽  
Tubulin Polymerization ◽  
Vinca Alkaloids ◽  
Antimitotic Activity ◽  
Transmission Electron ◽  
Flow Microfluorometry ◽  
Brain Tubulin

Maytansine, a novel ansa macrolide (1), has potent anti-tumor and antimitotic activity (2, 3). It blocks cell cycle traverse in mitosis with resultant accumulation of metaphase cells (4). Inhibition of brain tubulin polymerization in vitro by maytansine has also been reported (3). The C-mitotic effect of this drug is similar to that of the well known Vinca- alkaloids, vinblastine and vincristine. This study was carried out to examine the effects of maytansine on the cell cycle traverse and the fine struc- I ture of human lymphoblasts.Log-phase cultures of CCRF-CEM human lymphoblasts were exposed to maytansine concentrations from 10-6 M to 10-10 M for 18 hrs. Aliquots of cells were removed for cell cycle analysis by flow microfluorometry (FMF) (5) and also processed for transmission electron microscopy (TEM). FMF analysis of cells treated with 10-8 M maytansine showed a reduction in the number of G1 cells and a corresponding build-up of cells with G2/M DNA content.

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

scholarly journals A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles

1975 ◽  
Vol 148 (3) ◽  
pp. 533-537 ◽  
Author(s):  
R B Beechey ◽  
S A Hubbard ◽  
P E Linnett ◽  
A D Mitchell ◽  
E A Munn
Keyword(s):  
Electron Microscopy ◽  
Rapid Method ◽  
Adenosine Triphosphatase ◽  
Pure Form ◽  
Heart Mitochondria ◽  
Polyacrylamide Gels ◽  
Submitochondrial Particles ◽  
Bovine Heart

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.

Electron microscopy of rat liver cells in hypoxia

1960 ◽  
Vol 79 (1) ◽  
pp. 179-183 ◽  
Author(s):  
Maria Bassi ◽  
A. Bernelli-Zazzera ◽  
Elena Cassi
Keyword(s):  
Electron Microscopy ◽  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells

scholarly journals Potassium Organic Salts as Burn Additives in Cigarettes

2003 ◽  
Vol 20 (5) ◽  
pp. 341-347 ◽  
Author(s):  
C Liu ◽  
A Parry
Keyword(s):  
Electron Microscopy ◽  
Carbon Monoxide ◽  
Pressure Drop ◽  
Gradual Increase ◽  
Potassium Salts ◽  
Organic Salts ◽  
Salts Of Organic Acids ◽  
Wet Weight ◽  
Cigarette Ash ◽  
Scanning Electron

AbstractThree potassium salts of organic acids, namely malate, citrate and tartrate, have been sprayed onto flue-cured blend tobacco and subsequently tested for their performance as burn additives in cigarettes. In one experiment where potassium malate was added to vary the final tobacco potassium from ca. 3.1% to 8.3% (wet weight), an almost linearly reduction in puff temperature was measured. This was accompanied by a gradual increase in the cigarette's pressure drop. In another set of experiments where the final tobacco potassium contents were increased to ca. 5.1%, the three potassium salts showed almost equal reduction in the mainstream nicotine-free-dry-particulate-matter (NFDPM) at 32-35%, nicotine at 25-32% and carbon monoxide at 24-35%. Puff number showed ca. 23% increase with malate, 13% with citrate and almost unchanged for tartrate. Evidence of melting and coating by potassium malate was discovered in cigarette ash by scanning electron microscopy (SEM). This contributed to a noticeable change in ash morphology: small ash particles appeared to be coated and more tightly bonded together by the melt. This phenomenon was thought to be able to restrict the airflow during puffing, hence causing the measured increase in pressure drop, and the reductions in puff temperature, NFDPM, nicotine and carbon monoxide.

Simple, Sensitive Method for Measuring Plasmin and Plasminogen Activity in Plasma

1975 ◽  
Vol 21 (11) ◽  
pp. 1632-1637 ◽  
Author(s):  
Tetsuo Uete ◽  
Noriko Shimano ◽  
Mamiko Morikawa
Keyword(s):  
Rapid Method ◽  
Clinical Work ◽  
Sensitive Method

Abstract We describe a sensitive, simple, and rapid method for measuring plasmin and plasminogen in plasma, with fibrinogen as substrate. The assay can be done within 1 h. Plasma is diluted 20-fold with buffer and 0.1 ml (5 µl of original plasma) is incubated for 5 min at 37 °C, with or without 400 units of streptokinase. Then 2.0 ml of fibrinogen solution is added, the mixture again incubated (37 °C, 5 min), and the reaction stopped. The amount of tyrosine liberated from the fibrinogen is measured and is related to activity. This method is suitable for routine clinical work if the same batch of fibrinogen is used

A rapid method for the regional dissection of the rat brain

1980 ◽  
Vol 13 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Thomas G. Heffner ◽  
John A. Hartman ◽  
Lewis S. Seiden
Keyword(s):  
Rat Brain ◽  
Rapid Method
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