scholarly journals Temporal expression of membrane antigens during mouse spermatogenesis

1977 ◽  
Vol 74 (1) ◽  
pp. 86-97 ◽  
Author(s):  
CF Millete ◽  
AR Bellve
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Germ Cells ◽  
Surface Antigens ◽  
Spermatogenic Cells ◽  
Temporal Expression ◽  
Isolated Populations ◽  
Cell Surface Antigens ◽  
Membrane Antigens ◽  
Type A Spermatogonia

The temporal expression of cell surface antigens during mammalian spermatogenesis has been investigated using isolated populations of mouse germ cells. Spermatogenic cells at advanced stages of differentiation, including pachytene primary spermatocytes, round spermatids, and residual bodies of Regaud and mature spermatozoa, contain common antigenic membrane components which are not detected before the pachytene stage of the first meiotic prophase. These surface constituents are not detected on isolated populations of primitive type A spermatogonia, type A spermatogonia, type B spermatogonia, preleptotene primary spermatocytes, or leptotene and zygotene primary spermatocytes. These results have been demonstrated by immunofluorescence microscopy, by complement-mediated cytotoxicity, and by quantitative measurements of immunoglobulin (Ig) receptors on the plasma membrane of all cell populations examined. The cell surface antigens detected on germ cells are not found on mouse thymocytes, erythrocytes, or peripheral blood lymphocytes as determined by immunofluorescence and by cytotoxicity assays. Furthermore, absorption of antisera with kidney and liver tissue does not reduce the reactivity of the antibody preparations with spermatogenic cells, indicating that these antigenic determinants are specific to germ cells. This represents the first direct evidence for the ordered temporal appearance of plasma membrane antigens specific to particular classes of mouse spermatogenic cells. It appears that at late meiotic prophase, coincident with the production of pachytene primary spermatocytes, a variety of new components are inserted into the surface membranes of developing germ cells. The further identification and biochemical characterization of these constituents should facilitate an understanding of mammalian spermatogenesis at the molecular level.

Serologic Analyses of Cell-Surface Antigens ofAcanthamoebaspp. with Plasma Membrane Antisera*†

1977 ◽  
Vol 24 (2) ◽  
pp. 316-324 ◽  
Author(s):  
A. R. STEVENS ◽  
T. KILPATRICK ◽  
E. WILLAERT ◽  
A. CAPRONI
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Surface Antigens ◽  
Cell Surface Antigens

Orientation of cell-surface antigens in the lipid bilayer of lymphocyte plasma membrane

Nature ◽  
1977 ◽  
Vol 269 (5626) ◽  
pp. 307-311 ◽  
Author(s):  
Frank S. Walsh ◽  
Michael J. Crumpton
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Lipid Bilayer ◽  
Surface Antigens ◽  
Cell Surface Antigens

Isolation of plasma membranes from purified mouse spermatogenic cells

1980 ◽  
Vol 43 (1) ◽  
pp. 279-299
Author(s):  
C.F. Millette ◽  
D.A. O'Brien ◽  
C.T. Moulding
Keyword(s):  
Cell Surface ◽  
Germ Cells ◽  
Plasma Membranes ◽  
Biochemical Characterization ◽  
Lectin Binding ◽  
Round Spermatid ◽  
Ultrastructural Observation ◽  
Spermatogenic Cells ◽  
Cell Surface Antigens ◽  
Total Recovery

Plasma membranes have been prepared from purified pachytene spermatocytes, round spermatids and residual bodies of the adult mouse testis using procedures modified from other authors'. Isolated membranes have been examined using electron microscopy, lectin binding and enzymic assays. Ultrastructural observation reveals smooth unit-membrane vesicles from 0.4-1.7 micrometer diameter. No contamination by nuclei, mitochondria or lysosomes is detected microscopically. Radiolabelled lectin-binding experiments [125I-RCAI, 125I-green pea lectin] indicate that cell surface label cofractionates with material identified morphologically as plasma membrane. Estimates of total recovery of membrane, based upn the lectin data, average 33%. Biochemical analysis of subcellular markers reveal that no detectable DNA and only 1.2% of the total cellular RNA cofractionate with membranes. A variety of enzyme assays suggests little contamination by cytosol enzymes, Golgi material or mitochondria. Assays of 5′-nucleotidase (E.C. 3.1.3.5) indicate that this enzyme is not a major component of developing mouse spermatogenic cell membranes. Instead, Sertoli cells represent the most important source of this enzyme in the adult seminiferous tubule. Polyacrylamide gel analysis of membranes isolated from purified germ cells reveals significant differences in the protein compositions of pachytene spermatocyte and round spermatid membranes. The preparation of highly purified plasma membranes from homogeneous populations of spermatogenic cells should facilitate the biochemical characterization of cell surface antigens specific to developing male germ cells.

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

scholarly journals EFFECT OF 12-O-TETRADECANOYLPHORBOL-13-ACETATE ON EXPRESSION OF CELL SURFACE ANTIGENS IN TWO SUBCLONES OF HUMAN LEUKEMIA K562 CELLS

1993 ◽  
Vol 16 (10) ◽  
pp. 1054-1056
Author(s):  
Dai SASAKI ◽  
Satoshi KOSUNAGO ◽  
Takeshi MIKAMI ◽  
Tatsuji MATSUMOTO ◽  
Masuko SUZUKI
Keyword(s):  
Cell Surface ◽  
K562 Cells ◽  
Surface Antigens ◽  
Human Leukemia ◽  
Cell Surface Antigens ◽  
Human Leukemia K562 Cells

scholarly journals QUANTITATIVE STUDIES ON THE MIXED LYMPHOCYTE INTERACTION IN RATS

1971 ◽  
Vol 134 (4) ◽  
pp. 857-870 ◽  
Author(s):  
Darcy B. Wilson ◽  
Dianne H. Fox
Keyword(s):  
Cell Surface ◽  
Surface Antigens ◽  
Mouse Cell ◽  
Cell Surface Antigens ◽  
Quantitative Studies ◽  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures ◽  
Environmental Antigens ◽  
Germfree Rats ◽  
Number Of Cells

The proliferative reactivity of lymphocytes from rat donors maintained under germfree or conventional conditions was examined in mixed lymphocyte cultures stimulated with allogeneic and xenogeneic cell surface antigens. The results show (a) that lymphocytes from conventionally maintained rats are less reactive to human, hamster, guinea pig, and mouse cell surface antigens than to the major H alloantigens, and (b) that lymphocytes from germfree rats display no demonstrable reactivity to xenogeneic cells, but are quantitatively normal in their response to allogenic cells. The conclusion drawn from these observations is that the circulating lymphocyte pool of an individual consists of a greater proportion of cells reactive to H alloantigens of other members of the same species than to the xenogeneic cellular antigens of members of other species and that this large number of cells is not generated by a mechanism involving immunization to cross-reactive environmental antigens.

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