Characterization of Antibody Binding to Cell Surface Antigens Using a Plasma Membrane-Bound Plate Assay

1995 ◽  
Vol 224 (1) ◽  
pp. 39-50 ◽  
Author(s):  
C.A. Vater ◽  
K. Reid ◽  
L.M. Bartle ◽  
V.S. Goldmacher
Keyword(s):  
Plasma Membrane ◽  
Cell Surface ◽  
Surface Antigens ◽  
Antibody Binding ◽  
Cell Surface Antigens ◽  
Plate Assay ◽  
Membrane Bound

scholarly journals Detection and Characterization of Autoantibodies to Neuronal Cell-Surface Antigens in the Central Nervous System

2016 ◽  
Vol 9 ◽  
Author(s):  
Marleen H. van Coevorden-Hameete ◽  
Maarten J. Titulaer ◽  
Marco W. J. Schreurs ◽  
Esther de Graaff ◽  
Peter A. E. Sillevis Smitt ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Surface ◽  
Neuronal Cell ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
The Central Nervous System

scholarly journals Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody

2017 ◽  
Vol 114 (9) ◽  
pp. E1668-E1677 ◽  
Author(s):  
David Frescas ◽  
Christelle M. Roux ◽  
Semra Aygun-Sunar ◽  
Anatoli S. Gleiberman ◽  
Peter Krasnov ◽  
...  
Keyword(s):  
Cell Surface ◽  
Human Fibroblasts ◽  
Accelerated Aging ◽  
Lung Fibroblasts ◽  
Mouse Lung ◽  
Cell Surface Antigens ◽  
Cell Accumulation ◽  
Novel Biomarkers ◽  
Membrane Bound

Studying the phenomenon of cellular senescence has been hindered by the lack of senescence-specific markers. As such, detection of proteins informally associated with senescence accompanies the use of senescence-associated β-galactosidase as a collection of semiselective markers to monitor the presence of senescent cells. To identify novel biomarkers of senescence, we immunized BALB/c mice with senescent mouse lung fibroblasts and screened for antibodies that recognized senescence-associated cell-surface antigens by FACS analysis and a newly developed cell-based ELISA. The majority of antibodies that we isolated, cloned, and sequenced belonged to the IgM isotype of the innate immune system. In-depth characterization of one of these monoclonal, polyreactive natural antibodies, the IgM clone 9H4, revealed its ability to recognize the intermediate filament vimentin. By using 9H4, we observed that senescent primary human fibroblasts express vimentin on their cell surface, and MS analysis revealed a posttranslational modification on cysteine 328 (C328) by the oxidative adduct malondialdehyde (MDA). Moreover, elevated levels of secreted MDA-modified vimentin were detected in the plasma of aged senescence-accelerated mouse prone 8 mice, which are known to have deregulated reactive oxygen species metabolism and accelerated aging. Based on these findings, we hypothesize that humoral innate immunity may recognize senescent cells by the presence of membrane-bound MDA-vimentin, presumably as part of a senescence eradication mechanism that may become impaired with age and result in senescent cell accumulation.

Cell surface antigens on rat neural progenitors and characterization of the CD3 (+)/CD3 (−) cell populations

2006 ◽  
Vol 74 (9-10) ◽  
pp. 530-541 ◽  
Author(s):  
Solène Sergent-Tanguy ◽  
Joelle Véziers ◽  
Virginie Bonnamain ◽  
Hélène Boudin ◽  
Isabelle Neveu ◽  
...  
Keyword(s):  
Cell Surface ◽  
Surface Antigens ◽  
Neural Progenitors ◽  
Cell Populations ◽  
Cell Surface Antigens
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