scholarly journals The Morphology and Behavior of Living Exoerythrocytic Stages of Plasmodium gallinaceum and P. fallax and Their Host Cells

1960 ◽  
Vol 7 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Clay G. Huff ◽  
Alan C. Pipkin ◽  
A. B. Weathersby ◽  
Dinniemaud V. Jensen
Keyword(s):  
Phase Contrast ◽  
Nuclear Division ◽  
Time Lapse ◽  
Host Cells ◽  
Phase Contrast Microscopy ◽  
Tissue Cultures ◽  
Plasmodium Gallinaceum ◽  
Contrast Microscopy ◽  
And Behavior ◽  
Culture Phase

The morphology and behavior of living exoerythrocytic stages of Plasmodium gallinaceum and P. fallax were studied by the use of tissue cultures, phase contrast microscopy, and time-lapse cinephotomicrography. The morphology of exoerythrocytic stages of these two species was essentially that previously observed in fixed, stained material, with the following exceptions: (1) the presence of a filament on one end of the merozoite, (2) the absence of clefts in the cytoplasm of the large schizonts, and (3) the absence of a vacuole-like space around the parasite. The following behavior was observed either directly or in time-lapse sequences: (1) emergence of merozoites from mature schizonts, (2) progressive motility of free merozoites, (3) entry of merozoites, both actively and passively, into host cells, (4) nuclear division in the parasite, (5) the various stages of schizogony, including final production of merozoites, (6) massive infection of host cells, and (7) phagocytosis of merozoites and attempted phagocytosis of mature schizonts by macrophages. Exoerythrocytic stages of P. fallax differed from those of P. gallinaceum in that the merozoites of the former were (1) somewhat more curved in shape and (2) present in fewer numbers in mature schizonts. The use of tissue culture, phase contrast microscopy, and time-lapse cinephotomicrography promises to solve many of the remaining problems concerning exoerythrocytic stages of malarial parasites and their interrelationships with host cells.

Effect of microtubule inhibitors on the expansion of hypoblast and margin of overgrowth of chick blastoderms

Development ◽  
1984 ◽  
Vol 81 (1) ◽  
pp. 273-286
Author(s):  
Marc Mareel ◽  
Ruth Bellairs ◽  
Georges De Bruyne ◽  
Marie Christine Van Peteghem
Keyword(s):  
Phase Contrast ◽  
Time Lapse ◽  
Immunocytochemical Staining ◽  
Phase Contrast Microscopy ◽  
Chick Blastoderm ◽  
Cytoplasmic Microtubule ◽  
Artificial Substrata ◽  
Microtubule Inhibitors ◽  
Contrast Microscopy

The effect of the microtubule inhibitors, Nocodazole and taxol, was studied on the expansion of fragments of chick hypoblast (8 to 10 h incubation) and of margin of overgrowth (24 h incubation) cultured on artificial substrata and on the epiboly in intact blastoderms (24 h incubation). Immunocytochemical staining of these cells with antiserum against tubulin showed that 1 μg Nocodazole/ml caused disassembly of microtubules, and that 1 μg taxol/ml led to increased but unordered assembly. The solvent dimethylsulphoxide had no effect. At these concentrations both microtubule inhibitors led to rapid arrest of the expansion of fragments of hypoblast and of margin of overgrowth in culture, and of the epiboly in intact blastoderms. Time-lapse films showed that inhibition of expansion in both situations was reversible within 2 h after removal of the drugs. Phase-contrast microscopy showed remarkably little difference between the morphology of treated as compared to untreated cultures. Measurements of the height of the cells on sectioned fragments of margin of overgrowth showed no differences between treated and untreated cultures. These results suggest that the cytoplasmic microtubule complex is important both for epiboly and for the migration of hypoblast cells in the chick blastoderm. The mechanisms of this microtubule-related migration are not understood.

scholarly journals Time-lapse lens-free imaging of cell migration in diverse physical microenvironments

Lab on a Chip ◽  
2016 ◽  
Vol 16 (17) ◽  
pp. 3304-3316 ◽  
Author(s):  
Evelien Mathieu ◽  
Colin D. Paul ◽  
Richard Stahl ◽  
Geert Vanmeerbeeck ◽  
Veerle Reumers ◽  
...  
Keyword(s):  
Cell Migration ◽  
Live Cell Imaging ◽  
Phase Contrast ◽  
Cell Imaging ◽  
Time Lapse ◽  
Live Cell ◽  
Phase Contrast Microscopy ◽  
Contrast Microscopy ◽  
Coherent Illumination

Lens-free imaging using coherent illumination is established as an inexpensive and reliable alternative to conventional phase contrast microscopy for live-cell imaging applications.

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