scholarly journals INHIBITION OF DNA SYNTHESIS IN CHICK EMBRYO CULTURES BY DEPRIVATION OF EITHER SERUM OR ZINC

1973 ◽  
Vol 56 (3) ◽  
pp. 777-786 ◽  
Author(s):  
H. Rubin ◽  
T. Koide
Keyword(s):  
Chick Embryo ◽  
Population Density ◽  
Dna Synthesis ◽  
Cell Movement ◽  
Embryo Cultures ◽  
Low Concentrations ◽  
Embryo Cells ◽  
Inhibition Of Dna Synthesis ◽  
Kinetics Of ◽  
Kinetics Of Inhibition

The rate of DNA synthesis in cultures of chick embryo cells is proportional to the concentration of serum added. The concentration of serum required to stimulate DNA synthesis increases with cell population density and with the duration of culture after trypsinization. The increase of the serum requirement with population density is not caused by the depletion of serum constituents. The requirement of cells for external zinc in DNA synthesis also increases with population density and duration of culture. The kinetics of inhibition of DNA synthesis by deprivation of serum or zinc are similar. Serum deprivation, however, inhibits 2-deoxyglucose uptake and cell movement, but zinc deprivation does not. The deprivation of either serum or zinc inhibits RNA synthesis about twofold. Very low concentrations of actinomycin D prevent the resumption of RNA and DNA synthesis upon restoration of serum or zinc to deprived cultures.

scholarly journals K+/H+-antiporter nigericin arrests DNA synthesis in Ehrlich ascites carcinoma cells

1989 ◽  
Vol 86 (17) ◽  
pp. 6626-6629 ◽  
Author(s):  
L B Margolis ◽  
Y u Novikova I ◽  
I A Rozovskaya ◽  
V P Skulachev
Keyword(s):  
Membrane Potential ◽  
Dna Synthesis ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Low Concentrations ◽  
Ehrlich Ascites ◽  
Inhibition Of Dna Synthesis ◽  
Dna Synthesis Inhibition

Acidification of the cytoplasm of Ehrlich ascites carcinoma cells to pH 6.3 arrests DNA synthesis in these cells. Such an effect can be achieved by incubating the cells at pH 6.2 or by adding low concentrations of the K+/H+ antiporter, the antibiotic nigericin, at neutral pH. Glucose and anaerobiosis potentiate the nigericin effect. The inhibition of DNA synthesis by nigericin occurs without any significant decrease in the ATP concentration and in the mitochondrial membrane potential. The DNA synthesis inhibition is caused neither by a decrease in the intracellular [K+] nor by an increase in the intracellular [Na+] accompanying the nigericin effect (at least at low concentrations of the antibiotic). Nigericin should thus be regarded as a type of a cytostatic primarily affecting intracellular pH.

scholarly journals Characterization of a photoproduct of 7,12-dimethylbenz[a]anthracene and its effects on chick-embryo cells in culture

1977 ◽  
Vol 164 (3) ◽  
pp. 481-486 ◽  
Author(s):  
D Warshawsky ◽  
E Kerns ◽  
M J Bissell ◽  
M Calvin
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Oxidation Product ◽  
Biological Effects ◽  
Cell Number ◽  
Resonance Spectroscopy ◽  
Morphological Transformation ◽  
Morphological Alterations ◽  
Photo Oxidation ◽  
Embryo Cells

A common impurity of 7,12-dimethylbenz[alpha]anthracene was more effective than 7,12-dimethylbenz[alpha]anthracene in inducing morphological alterations, and in causing an increase in glucose uptake, DNA synthesis and cell number in chick-embryo fibroblasts. Gradual morphological transformation follows the increase in DNA synthesis after 2 days when either primary or secondary cultures are treated with 3 microgram of the compound/ml. The compound, isolated from 7,12-dimethylbenz[alpha]anthracene by alumina column chromatography, was characterized by t.l.c., mass spectroscopy, carbon-hydrogen analysis, u.v. and nuclear-magnetic-resonance spectroscopy and thermal decomposition. It was the photo-oxidation product of 7,12-dimethylbenz[alpha]anthracene, 7,12-epidioxy-7,12-dimethylbenz[alpha]anthracene. It is suggested that some of the biological effects observed after treatment of cultures with 7,12-dimethylbenz[alpha]anthracene may be due in part to the presence of the photo-oxidation product.

Der Einfluß von 4-Hydroxy-2.3-pentenal (HPE) auf die Vermehrung von Vaccinia-Virus in Hühnerfibroblastenkulturen / Influence of 4-Hydroxy-2,3-pentenale (HPE) on the Multiplication of Vaccinia-virus in Chick-embryo Fibroblast Cultures

1974 ◽  
Vol 29 (1-2) ◽  
pp. 76-81 ◽  
Author(s):  
V. Dostal ◽  
E. Schauenstein ◽  
P. Kulnigg ◽  
E. Schmeller
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Vaccinia Virus ◽  
Normal Cell ◽  
Thymidine Incorporation ◽  
Selective Inhibition ◽  
Virus Concentration ◽  
Chick Embryo Fibroblast ◽  
Fibroblast Cultures ◽  
Embryo Cultures

Abstract The influence of 4-hydroxy-2,3-/rans-pentenale (HPE) on multiplication of Vaccinia virus in chick fibroblast cultures has been investigated. The cytotoxicity of HPE has been estimated by in ­ corporation of [3H] thymidine, [3H] uridine, [3H] leucine and by morphological tests. The greatest influence of H PE was seen after incorporation of [3H] thymidine. Chick-embryo cultures infected with Vaccinia virus showed a marked increase of DNA synthesis in contrast to the controls. This increase averaged 180% after 16 to 14 hours. HPE 0.05-10_ 3 M caused a reduction of 72% in [3H] thymidine incorporation in virus infected cultures. At the same time virus concentration decreased by 76%. HPE 0.1-10~3 M, a concentration which inhibits the normal cell DNA synthesis by 50%, caused about 90% inhibition in infected cultures; virus m ulti­ plication was 99% inhibited. These findings have been attributed to a selective inhibition of virus DNA synthesis by HPE. and possible mechanisms are discussed.

scholarly journals Differential effect of hormones on macromolecular synthesis and mitosis in chick embryo cells.

1975 ◽  
Vol 67 (2) ◽  
pp. 492-498 ◽  
Author(s):  
J B Baseman ◽  
N S Hayes
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Metabolic Responses ◽  
Resting Cells ◽  
Cell Heterogeneity ◽  
Macromolecular Synthesis ◽  
Maximal Stimulation ◽  
Fresh Serum ◽  
Embryo Cells ◽  
Stimulation Of

Exposure of serum-deprived confluent monolayers of chick embryo cells to fresh serum results in maximal stimulation of synthesis of RNA and protein followed by increased DNA synthesis and mitosis. The addition of insulin to quiescent cultures effects a similar acceleration of synthesis of RNA and protein, but little stimulation of DNA synthesis and mitosis is evident. However, the simultaneous addition of insulin and hydrocortisone to resting cells causes a significant increase in the rate of DNA synthesis although the level reached is considerably lower than that obtained with serum and still no mitosis occurs. Unexpectedly, insulin plus hydrocortisone prevents maximal synthesis of RNA and protein in contrast to insulin-treated cultures. Nuclear autoradiography and percent mitosis of cells incubated with various regulatory factors indicate that cell heterogeneity exists and is reflected in the metabolic responses of subpopulations to specific regulatory signals.

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