scholarly journals Characterization of a photoproduct of 7,12-dimethylbenz[a]anthracene and its effects on chick-embryo cells in culture

1977 ◽  
Vol 164 (3) ◽  
pp. 481-486 ◽  
Author(s):  
D Warshawsky ◽  
E Kerns ◽  
M J Bissell ◽  
M Calvin
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Oxidation Product ◽  
Biological Effects ◽  
Cell Number ◽  
Resonance Spectroscopy ◽  
Morphological Transformation ◽  
Morphological Alterations ◽  
Photo Oxidation ◽  
Embryo Cells

A common impurity of 7,12-dimethylbenz[alpha]anthracene was more effective than 7,12-dimethylbenz[alpha]anthracene in inducing morphological alterations, and in causing an increase in glucose uptake, DNA synthesis and cell number in chick-embryo fibroblasts. Gradual morphological transformation follows the increase in DNA synthesis after 2 days when either primary or secondary cultures are treated with 3 microgram of the compound/ml. The compound, isolated from 7,12-dimethylbenz[alpha]anthracene by alumina column chromatography, was characterized by t.l.c., mass spectroscopy, carbon-hydrogen analysis, u.v. and nuclear-magnetic-resonance spectroscopy and thermal decomposition. It was the photo-oxidation product of 7,12-dimethylbenz[alpha]anthracene, 7,12-epidioxy-7,12-dimethylbenz[alpha]anthracene. It is suggested that some of the biological effects observed after treatment of cultures with 7,12-dimethylbenz[alpha]anthracene may be due in part to the presence of the photo-oxidation product.

scholarly journals Differential effect of hormones on macromolecular synthesis and mitosis in chick embryo cells.

1975 ◽  
Vol 67 (2) ◽  
pp. 492-498 ◽  
Author(s):  
J B Baseman ◽  
N S Hayes
Keyword(s):  
Chick Embryo ◽  
Dna Synthesis ◽  
Metabolic Responses ◽  
Resting Cells ◽  
Cell Heterogeneity ◽  
Macromolecular Synthesis ◽  
Maximal Stimulation ◽  
Fresh Serum ◽  
Embryo Cells ◽  
Stimulation Of

Exposure of serum-deprived confluent monolayers of chick embryo cells to fresh serum results in maximal stimulation of synthesis of RNA and protein followed by increased DNA synthesis and mitosis. The addition of insulin to quiescent cultures effects a similar acceleration of synthesis of RNA and protein, but little stimulation of DNA synthesis and mitosis is evident. However, the simultaneous addition of insulin and hydrocortisone to resting cells causes a significant increase in the rate of DNA synthesis although the level reached is considerably lower than that obtained with serum and still no mitosis occurs. Unexpectedly, insulin plus hydrocortisone prevents maximal synthesis of RNA and protein in contrast to insulin-treated cultures. Nuclear autoradiography and percent mitosis of cells incubated with various regulatory factors indicate that cell heterogeneity exists and is reflected in the metabolic responses of subpopulations to specific regulatory signals.

scholarly journals INHIBITION OF DNA SYNTHESIS IN CHICK EMBRYO CULTURES BY DEPRIVATION OF EITHER SERUM OR ZINC

1973 ◽  
Vol 56 (3) ◽  
pp. 777-786 ◽  
Author(s):  
H. Rubin ◽  
T. Koide
Keyword(s):  
Chick Embryo ◽  
Population Density ◽  
Dna Synthesis ◽  
Cell Movement ◽  
Embryo Cultures ◽  
Low Concentrations ◽  
Embryo Cells ◽  
Inhibition Of Dna Synthesis ◽  
Kinetics Of ◽  
Kinetics Of Inhibition

The rate of DNA synthesis in cultures of chick embryo cells is proportional to the concentration of serum added. The concentration of serum required to stimulate DNA synthesis increases with cell population density and with the duration of culture after trypsinization. The increase of the serum requirement with population density is not caused by the depletion of serum constituents. The requirement of cells for external zinc in DNA synthesis also increases with population density and duration of culture. The kinetics of inhibition of DNA synthesis by deprivation of serum or zinc are similar. Serum deprivation, however, inhibits 2-deoxyglucose uptake and cell movement, but zinc deprivation does not. The deprivation of either serum or zinc inhibits RNA synthesis about twofold. Very low concentrations of actinomycin D prevent the resumption of RNA and DNA synthesis upon restoration of serum or zinc to deprived cultures.

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

scholarly journals Incorporation of Leucine-C14 into a Virus-specific Protein in Homogenates of Chick Embryo Cells

1960 ◽  
Vol 235 (3) ◽  
pp. 660-664
Author(s):  
Gerald C. Mueller ◽  
Sigrid Von Zahn-Ullmann ◽  
Werner Schäfer
Keyword(s):  
Chick Embryo ◽  
Specific Protein ◽  
Embryo Cells

scholarly journals EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE

1974 ◽  
Vol 61 (2) ◽  
pp. 369-382 ◽  
Author(s):  
D. A. Newsome ◽  
R. T. Fletcher ◽  
W. G. Robison ◽  
K. R. Kenyon ◽  
G. J. Chader
Keyword(s):  
Molecular Weight ◽  
Chick Embryo ◽  
Adenosine Monophosphate ◽  
Cell Number ◽  
Retinal Pigmented Epithelium ◽  
Culture Dish ◽  
Adhesive Properties ◽  
Embryo Extract ◽  
Chick Embryo Extract ◽  
Pigmented Epithelium

The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated. Seven cloned PE cell lines were each grown in modified Ham's F-12 medium alone (F-12), or in F-12 supplemented with either high molecular weight (H) or low molecular weight (L) fractions of chick embryo extract. Cells grown in F-12 alone or in L medium formed compact epithelial sheets, whereas cells grown in H had a fibrocytic appearance and formed poorly organized monolayers. In H plus BcAMP, cell morphology was more epithelioid than in H alone, and generally the monolayers appeared more differentiated. Under each of these three culture conditions, 2 x 10-4 M BCAMP retarded the increase in cell number and decreased the final number of cells per culture dish, but had little effect on plating efficiency. BcAMP also increased the rate of cell adhesion to a plastic substratum. Pigmentation was marked in cultures grown in F-12 or in L alone, but the addition of BcAMP dramatically reduced visible pigmentation. This effect was reversed when BcAMP was removed from the culture medium. Thus BcAMP modifies cell and colonial morphology, rate of cell accumulation, adhesive properties, and pigmentation of normal PE cells.

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