scholarly journals FERRITIN PARTICLES IN MACROPHAGES AND IN ASSOCIATED MAST CELLS

1972 ◽  
Vol 52 (3) ◽  
pp. 536-541 ◽  
Author(s):  
J. V. Simson ◽  
S. S. Spicer
Keyword(s):  
Lymph Node ◽  
Mast Cells ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Light Microscope Level ◽  
Reactive Material ◽  
Reactive Substance ◽  
Cytoplasmic Vacuoles ◽  
Intimate Association ◽  
The Matrix

In a variety of tissues (lymph node and glandular stroma), mast cells have been found in close and often intimate association with macrophages containing numerous ferritin-like particles in their cytoplasm and within cytoplasmic vacuoles (siderosomes). Phagocytic vacuoles in a given macrophage differed markedly. Some contained abundant Prussian blue-reactive material and others contained periodic acid-Schiff reactive substance at the light microscope level, and ultrastructurally some were filled with ferritin particles and others were not. Ferritin-like particles have also been observed occasionally in the mast cells associated with macrophages and even within the matrix of some of the granules in these mast cells.

The Tinctorial Behaviour of Human Mast Cells

1954 ◽  
Vol s3-95 (29) ◽  
pp. 1-4 ◽  
Author(s):  
WILLIAM MONTAGNA ◽  
ARTHUR Z. EISEN ◽  
ALLEN S. GOLDMAN
Keyword(s):  
Mast Cells ◽  
Blood Vessels ◽  
Toluidine Blue ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Running Water ◽  
Enzyme Preparations ◽  
Reactive Substance ◽  
Ph Values ◽  
Blue Stain

Mast cells in the skin differentiate from perivascular fibroblasts. The cells nearest the walls of the blood-vessels contain mostly sparse and small mast granules; in those farther removed from the blood-vessels the granules are more numerous and coarse. With weak solutions of toluidine blue, mast granules reveal maximal chromotropy at pH 5-0. At lower pH values not all of the granules stain; at higher ones the granules and the intergranular cytoplasm stain progressively more orthochromatically. After digestion with ribonuclease and staining with toluidine blue buffered to pH 4.0 or 5.0 the mast granules are cherry red and all traces of orthochromatic staining are abolished; when stained at pH 60 or above, however, the cytoplasm and the granules attain a strong blue stain as if they had not been digested in the enzyme. Preparations fixed in Helly's fluid may be washed in running water overnight and the mast granules show no diminution in chromotropy. The same sections may be stained, destained, and stained again at any desired pH with excellent results. Both the cytoplasm and the granules are Schiff-reactive, but the granules stain more intensely than the background. Sections stained with the periodic acid/Schiff technique and subsequently stained with toluidine blue reveal the mast granules brilliantly metachromatic, suggesting that the metachromatic and the Schiff-reactive substances, although coexistent, may be in fact separate elements. Mast granules, according to these tinctorial reactions, then, may contain 4 substances: (a) a protein cytoskeleton stainable with toluidine blue buffered to pH 6.0 or above; (b) some ribonucleic acid removable with ribonuclease and stainable with toluidine blue buffered to pH 5.0 or below; (c) an acid mucopolysaccharide which stains metachromatically; and (d) a Schiff-reactive substance.

Systemic Mastocytosis in a Goat

1995 ◽  
Vol 32 (6) ◽  
pp. 719-721 ◽  
Author(s):  
K. N. M. Khan ◽  
J. E. Sagartz ◽  
G. Koenig ◽  
K. Tanaka
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Transmission Electron Microscopy ◽  
Mast Cells ◽  
Systemic Mastocytosis ◽  
Periodic Acid ◽  
Hypochromic Anemia ◽  
Postmortem Examination ◽  
Periodic Acid Schiff ◽  
Transmission Electron

Systemic mastocytosis was diagnosed in a 4-year-old, female Nubian goat. Clinically, the animal was depressed and had severe macrocytic hypochromic anemia and leukopenia. Postmortem examination revealed neoplastic mast cells invading the heart, lung, liver, spleen, lymph nodes, and bone marrow. Eosinophils were frequently admixed with infiltrating mast cells in all organs. Using routine light microscopy, histochemistry, and transmission electron microscopy, metachromatic and periodic acid—Schiff–positive granules were identified within the cytoplasm of neoplastic mast cells. Erythrophagocytosis was observed in some neoplastic cells, although its contribution to the anemia was not clear. This report represents the first description of mast cell neoplasia in the goat.

scholarly journals The presence of mast cell precursors in rat peripheral blood

Blood ◽  
1981 ◽  
Vol 58 (3) ◽  
pp. 544-551 ◽  
Author(s):  
D Zucker-Franklin ◽  
G Grusky ◽  
N Hirayama ◽  
E Schnipper
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peripheral Blood ◽  
Periodic Acid ◽  
Phenotypic Expression ◽  
Soft Agar ◽  
Sulfated Polysaccharides ◽  
Periodic Acid Schiff ◽  
Agar Culture

Abstract Soft agar culture of mononuclear cell fractions prepared from rat peripheral blood yielded numerous colonies consisting of mast cells. The mast cell nature of the cells was established by ultrastructural and histochemical analyses as well as by the demonstration the the colonies contained histamine and that the cells possessed receptors for the Fc component of IgE. Stringent criteria for the distinction of mast cells from monocytes/macrophages that could have metachromatic inclusions were applied. The alcian-blue-safranin technique delineated the maturation of mast cell granules by showing the loss of alcian-blue and increase in safranin-positive organelles presumed to reflect the increase in N-sulfated polysaccharides representing heparin. The mast cells exhibited low or absent reactions for peroxidase, alpha-naphthyl butyrate, periodic acid Schiff, and Sudan black reacting lipid, whereas macrophages stained in parallel were positive for these substances. Since it is known that extracellular conditions may cause variations in phenotypic expression, the observations have led to the hypothesis that mast cells and macrophages may have a common precursor.

Prognostic Significance of Periodic Acid-Schiff-Positive Patterns in Primary Breast Cancer and its Lymph Node Metastases

2004 ◽  
Vol 84 (2) ◽  
pp. 117-130 ◽  
Author(s):  
Jeroen T. Buijs ◽  
Anne-Marie Cleton ◽  
Vincent T.H.B.M. Smit ◽  
Clemens W.G.M. Löwik ◽  
Socrates E. Papapoulos ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastases ◽  
Primary Breast Cancer ◽  
Periodic Acid ◽  
Prognostic Significance ◽  
Periodic Acid Schiff ◽  
Node Metastases

scholarly journals NORMAL HUMAN NEUTROPHIL LEUKOCYTES AS A REFERENCE SYSTEM FOR THE MICROSPECTROPHOTOMETRICALLY QUANTITATED PERIODIC ACID-SCHIFF REACTION

1966 ◽  
Vol 14 (1) ◽  
pp. 45-48 ◽  
Author(s):  
GÖSTA GAHRTON
Keyword(s):  
Human Neutrophil ◽  
Reference System ◽  
Periodic Acid ◽  
Reference Series ◽  
Periodic Acid Schiff ◽  
Reactive Material ◽  
Pas Reaction ◽  
Normal Human ◽  
Schiff Reaction ◽  
Neutrophil Leukocytes

Normal human neutrophil leukocytes are suggested as references for a microspectrophotometrically quantitated PAS reaction in biological material. Analysis of variance of values for the total extinction at 546 mµ in PAS-stained, single, normal neutrophils proved these cells to be suitable references. Methanol-fixed, smeared neutrophils could be stored for several months without changing their amount of PAS reactive material. A person with a mean for the amount of PAS reactive material in neutrophils that lay close to the average in a normal material of 20 persons was chosen as the original reference subject. New reference series of neutrophils were related to the original one, thus obtaining a continuous reference system. The results are based upon microspectrophotometric measurements of about 3500 individual neutrophils.

scholarly journals EXTRACTION RESISTANCE OF SUDAN STAINED MAST CELLS AFTER PREVIOUS ACID TREATMENT

1964 ◽  
Vol 12 (7) ◽  
pp. 530-532 ◽  
Author(s):  
JOHN R. FEAGLER ◽  
J. F. A. MCMANUS
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Chemical Property ◽  
Cell Nucleus ◽  
Acid Treatment ◽  
Periodic Acid ◽  
Hydrogen Ion ◽  
Periodic Acid Schiff ◽  
Sudan Black B ◽  
Sudan Dye

1. Sudan black B alone stains the mast cells very faintly. The granules do not appear very distinct or numerous. 2. The periodic acid Schiff (PAS) technique stains the mast cells; however, the results are variable. When stained, the mast cell granules appear slightly darker than the cytoplasm. 3. When PAS and Sudan black are used together, the mast cell granules gain an avidity for the dye and are stained black. 4. Brief acid treatment prior to immersion in Sudan black results in granules gaining an avidity for the Sudan dye that is extraction resistant. The reaction appears dependent upon modifying the mast cell granules by acid treatment. It appears as though the hydrogen ion changes some physical or chemical property of the granules so that they gain an avidity for the dye. 5. Acetylated Sudan black shows an affinity for the mast cell granules greater than Sudan black alone. This is not increased by previous acid treatment. Acetylated Sudan black also stains the mast cell nucleus.

scholarly journals The presence of mast cell precursors in rat peripheral blood

Blood ◽  
1981 ◽  
Vol 58 (3) ◽  
pp. 544-551
Author(s):  
D Zucker-Franklin ◽  
G Grusky ◽  
N Hirayama ◽  
E Schnipper
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peripheral Blood ◽  
Periodic Acid ◽  
Phenotypic Expression ◽  
Soft Agar ◽  
Sulfated Polysaccharides ◽  
Periodic Acid Schiff ◽  
Agar Culture

Soft agar culture of mononuclear cell fractions prepared from rat peripheral blood yielded numerous colonies consisting of mast cells. The mast cell nature of the cells was established by ultrastructural and histochemical analyses as well as by the demonstration the the colonies contained histamine and that the cells possessed receptors for the Fc component of IgE. Stringent criteria for the distinction of mast cells from monocytes/macrophages that could have metachromatic inclusions were applied. The alcian-blue-safranin technique delineated the maturation of mast cell granules by showing the loss of alcian-blue and increase in safranin-positive organelles presumed to reflect the increase in N-sulfated polysaccharides representing heparin. The mast cells exhibited low or absent reactions for peroxidase, alpha-naphthyl butyrate, periodic acid Schiff, and Sudan black reacting lipid, whereas macrophages stained in parallel were positive for these substances. Since it is known that extracellular conditions may cause variations in phenotypic expression, the observations have led to the hypothesis that mast cells and macrophages may have a common precursor.

CHANGES IN THE BONE AND CARTILAGE OF THE SYMPHYSIS PUBIS OF THE MOUSE DURING PREGNANCY AND AFTER PARTURITION, AS REVEALED BY METACHROMATIC STAINING AND THE PERIODIC ACID-SCHIFF TECHNIQUE

1954 ◽  
Vol 11 (2) ◽  
pp. 210-NP ◽  
Author(s):  
KATHLEEN HALL
Keyword(s):  
Connective Tissue ◽  
Endochondral Ossification ◽  
Post Partum ◽  
Periodic Acid ◽  
Symphysis Pubis ◽  
Periodic Acid Schiff ◽  
Histological Changes ◽  
The Matrix ◽  
Haemopoietic Cells ◽  
Chondroid Matrix

SUMMARY A re-investigation has been made of the histological changes in the tissues of the symphysis pubis of the mouse during the first pregnancy and after parturition, using the techniques of metachromatic staining with toluidine blue, the McManus-Hotchkiss periodic acid-Schiff (p.a.s.) reaction, and basophilic staining with haematoxylin. During the last week of pregnancy two maj or changes take place: (1) resorption of the anteromedial bony walls of the innominates leading to shortening of the symphysis, and (2) transformation of the symphysial cartilage, manifested by loss of metachromasia and basophilia and ability to react with p.a.s., leading to ultimate disappearance of stainable chondroid matrix and its replacement first by argyrophil and then by collagenous connective tissue. Further resorption of bone from the symphysial walls of the innominates and, probably, retention of fluid in the newly formed connective tissue helps to widen the interpubic gap. After parturition, the collagenous connective tissue which had replaced the cartilage and resorbed bone reverts to a mesenchyme-like tissue, the cells of which differentiate into osteoblasts or chondroblasts or haemopoietic cells. Cartilage differentiates by deposition of metachromatic chondroid matrix around chondroblasts, the matrix becoming more and more metachromatic, p.a.s.-positive and basophilic. Bone differentiates, first in connective tissue, later by endochondral ossification. Marrow begins to differentiate even before the bone which will confine it is laid down. The effect of hyaluronidase on metachromasia, basophilia and reaction to p.a.s. of the symphysial tissues is described, and its significance discussed in relation to the pregnancy and post-partum changes.

scholarly journals Immunohistochemical characterisation of a canine case of granular cell type trichoblastoma: a case report

2014 ◽  
Vol 59 (No. 10) ◽  
pp. 511-514
Author(s):  
JS Yoon ◽  
J. Park
Keyword(s):  
Periodic Acid ◽  
Granular Cell ◽  
Stem Cell Marker ◽  
Cell Type ◽  
Intact Male ◽  
Periodic Acid Schiff ◽  
Cytoplasmic Staining ◽  
Fibrous Stroma ◽  
Clear Cells ◽  
Cytoplasmic Vacuoles

Herein we describe a case of granular cell type trichoblastoma in a dog. A nine-year-old, intact male Scottish terrier presented with a solitary, exophytic and non-ulcerated nodule on the shoulder. Histopathologically, the nodule showed a ribbon-like structure, which was composed of branching cords of epithelial cells surrounded by fibrous stroma. In addition, islands of large clear cells were also noted. The clear cells showed eccentric nuclei and abundant cytoplasmic vacuoles, which were strongly positive for periodic acid-Schiff staining. Cytoplasmic immunostaining for cytokeratin (CK) 14 and nuclear immunostaining for p63 were observed in the neoplastic cells. In contrast, CK18 staining was scant. Furthermore, the hair bulge stem cell marker CK15 showed strong cytoplasmic staining. Based on these histopathological and immunohistochemical findings, the tumour was diagnosed as a granular cell type trichoblastoma, which is rarely reported in dogs.  

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