scholarly journals PASSAGE OF FUCOSE-3H LABEL FROM THE GOLGI APPARATUS INTO DENSE AND MULTIVESICULAR BODIES IN THE DUODENAL COLUMNAR CELLS AND HEPATOCYTES OF THE RAT

1971 ◽  
Vol 51 (3) ◽  
pp. 875-881 ◽  
Author(s):  
G. Bennett ◽  
C. P. Leblond
Keyword(s):  
Golgi Apparatus ◽  
Multivesicular Bodies ◽  
Columnar Cells

scholarly journals Langerhans' Cells in Equine Cutaneous Papillomas and Normal Skin

1992 ◽  
Vol 29 (2) ◽  
pp. 152-160 ◽  
Author(s):  
M. Hamada ◽  
M. Takechi ◽  
C. Itakura
Keyword(s):  
Major Histocompatibility Complex ◽  
T Lymphocytes ◽  
Golgi Apparatus ◽  
Langerhans Cells ◽  
Normal Skin ◽  
Multivesicular Bodies ◽  
Major Histocompatibility Complex Antigen ◽  
Histocompatibility Complex ◽  
Thoroughbred Horses ◽  
Normal Epidermis

Langerhans' cells (LC) were investigated immunohistochemically and electron microscopically in normal equine epidermis and 133 equine cutaneous papillomas experimentally induced in five 2-year-old Thoroughbred horses. Class II major histocompatibility complex antigen-positive dendritic LC were found in the normal epidermis and ultrastructurally had the characteristic Birbeck's granules. In the developing phase of the papillomas, LC were significantly decreased in number and size, indicative of a hypofunctional state. In the regressing phase of the papillomas, LC were markedly increased in number, especially at the epidermis-dermis junction. LC with long dendrites were rich in cytoplasm with well-developed cytoplasmic organelles, including Golgi apparatus, lysosomes, Birbeck's granules, and multivesicular bodies. These LC were hyperfunctional. An infiltration of many T lymphocytes was also observed at the epidermis-dermis junction.

scholarly journals Autoradiographic demonstration of in vivo sialylation of endogenous acceptors at the microvillar surface of intestinal columnar cells after intraluminal administration of CMP-[3H]-sialic acid.

1987 ◽  
Vol 35 (8) ◽  
pp. 861-864 ◽  
Author(s):  
G Bennett ◽  
A Haddad ◽  
C P Leblond
Keyword(s):  
Small Intestine ◽  
Sialic Acid ◽  
Epithelial Cells ◽  
Golgi Apparatus ◽  
Posterior Chamber ◽  
Rat Jejunum ◽  
Sialyltransferase Activity ◽  
Columnar Cells

To investigate the presence of glycosyltransferase activity at the apical surfaces of columnar cells in small intestine, CMP-[3H]-sialic acid was injected into the lumen of a ligated segment of rat jejunum; 5 min later the tissue was fixed and processed for light microscopic autoradiography. After a 3-6-month exposure, an autoradiographic reaction appeared over the microvillar surfaces of columnar cells, indicating the presence of surface sialyltransferase activity accompanied by endogenous acceptors. When CMP-[3H]-sialic acid was injected into the posterior chamber of rat eye or the lumen of mouse gallbladder, no autoradiographic reaction was observed at the surfaces of the cells facing these cavities. After injection of UDP-[3H]-galactose into the same three sites, an autoradiographic reaction was observed in the Golgi regions of the various epithelial cells, but not along their apical surfaces. Competition experiments using unlabeled galactose indicated that [3H]-galactose had been released from the nucleotide and had entered the cells to be incorporated into the Golgi apparatus.

scholarly journals Stress-Induced Intracellular Trafficking of Corticotropin-Releasing Factor Receptors in Rat Locus Coeruleus Neurons

Endocrinology ◽  
2007 ◽  
Vol 149 (1) ◽  
pp. 122-130 ◽  
Author(s):  
Beverly A. S. Reyes ◽  
Rita J. Valentino ◽  
Elisabeth J. Van Bockstaele
Keyword(s):  
Plasma Membrane ◽  
Golgi Apparatus ◽  
Locus Coeruleus ◽  
Intracellular Trafficking ◽  
Corticotropin Releasing Factor ◽  
Cellular Trafficking ◽  
Multivesicular Bodies ◽  
Swim Stress ◽  
Early Endosomes ◽  
Crf1 Antagonist

Corticotropin-releasing factor (CRF) activates locus coeruleus (LC)-norepinephrine neurons during stress. Previous stress or CRF administration attenuates the magnitude of this response by decreasing postsynaptic sensitivity to CRF. Here we describe the fate of CRF receptors (CRFr) in LC neurons after stress. Rats were exposed to swim stress or handling and perfused 1 or 24 h later. Sections through the LC were processed for immunogold-silver labeling of CRFr. CRFr in LC dendrites was present on the plasma membrane and within the cytoplasm. In control rats, the ratio of cytoplasmic to total dendritic labeling was 0.55 ± 0.01. Swim stress increased this ratio to 0.77 ± 0.01 and 0.80 ± 0.02 at 1 and 24 h after stress, respectively. Internalized CRFr was associated with different organelles at different times after stress. At 1 h after stress, CRFr was often associated with early endosomes in dendrites and perikarya. By 24 h, more CRFr was associated with multivesicular bodies, suggesting that some of the internalized receptor is targeted for degradation. In perikarya, more internalized CRFr was associated with Golgi apparatus 24 vs. 1 h after stress. This is suggestive of changes in CRFr synthesis. Alternatively, this may indicate communication between multivesicular bodies and Golgi apparatus in the process of recycling. Administration of the selective CRF1 antagonist, antalarmin, before swim stress attenuated CRFr internalization. The present demonstration of stress-induced internalization of CRFr in LC neurons provides evidence that CRF is released in the LC during swim stress to activate this system and initiate cellular trafficking of the receptor that determines subsequent sensitivity of LC neurons to CRF.

Sur la différenciation des trachéides dans les haustoriums d'Osyris alba parasitant Hedera helix

1987 ◽  
Vol 65 (8) ◽  
pp. 1746-1755 ◽  
Author(s):  
H. Benharrat ◽  
S. Renaudin ◽  
L. Rey ◽  
P. Thalouarn
Keyword(s):  
Golgi Apparatus ◽  
Secondary Wall ◽  
Multivesicular Bodies ◽  
Tracheary Elements ◽  
Hedera Helix ◽  
Second Stage ◽  
Host Root ◽  
Cytoplasmic Content ◽  
Cambial Cells ◽  
Surrounding Membrane

The central part of the haustorium of Osyris alba L. contains a cambium which is continuous from the mother root to the vicinity of the "absorbing cells." It centripetally differentiates tracheary elements which make up a vascular core, the xylem bridge, connecting the vessels of the parasite root to those of the host root. In the differentiating cambial cells, a first stage of activity of the Golgi apparatus, which is related to the development of the secondary wall thickenings, is evident. This stage is marked by the presence of numerous vesicles containing an electron dense material and of multivesicular bodies lying near the plasmalemma. The cells also contain numerous spherical granules which, while being mainly proteinaceous, also contain some potassium, calcium, and sulfur. The composition of these granules, the fact that their surrounding membrane is studded with numerous ribosomes and the fact that they often form short chains in a common membranous profile lead us to conclude that they are elaborated in the endoplasmic reticulum. Later in the course of differentiation of those cells, a second stage of activity of the Golgi apparatus can be observed, with the numerous vesicles showing a light content this time. This phase precedes a lysis process during which the cells lose all their cytoplasmic content. Only the granules remain in the differentiated tracheids. [Journal translation]

scholarly journals CYTOCHEMICAL STUDIES OF LYSOSOMES, GOLGI APPARATUS AND ENDOPLASMIC RETICULUM IN SECRETION AND PROTEIN UPTAKE BY ADRENAL MEDULLA CELLS OF THE RAT

1968 ◽  
Vol 16 (5) ◽  
pp. 320-336 ◽  
Author(s):  
ERIC HOLTZMAN ◽  
REGINA DOMINITZ
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Golgi Apparatus ◽  
Adrenal Medulla ◽  
Secretory Granules ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Frozen Sections ◽  
Protein Uptake ◽  
Thiamine Pyrophosphatase

The adrenalin-producing cells of the rat adrenal medulla have been studied by light and electron microscopy. Frozen sections of glutaraldehyde-perfused material were incubated for demonstration of "marker" enzymes for lysosomes (acid phosphatase, aryl sulfatase) and Golgi apparatus (thiamine pyrophosphatase). In addition, the uptake and fate of intravenously administered horseradish peroxidase was followed. Acid phosphatase activity is demonstrable in secretory granules, Golgi saccules, vesicles in the Golgi area and in the agranular tubules and cisternae (GERL) from which secretory granules appear to form at the inner surface of the Golgi apparatus. Endoplasmic reticulum with ribosomes on only one surface is closely apposed to both inner and outer aspects of the Golgi apparatus. Peroxidase is taken up in vesicles, tubules and "cup-like" bodies. The latter apparently transform into multivesicular bodies. A possible source of the acid phosphatase found in multivesicular bodies is the small vesicles from the Golgi apparatus or GERL.

Memoirs: Cytology of Human Uterine Glands in Gravid and Mon-Gravid Phases

1941 ◽  
Vol s2-82 (328) ◽  
pp. 541-562
Author(s):  
OLIVE E. AYKROYD ◽  
J. BRONTË GATENBY
Keyword(s):  
Golgi Apparatus ◽  
Secretory Cell ◽  
Outer Edge ◽  
Pregnancy Test ◽  
Proliferative Phase ◽  
Uterine Glands ◽  
Columnar Cells

1. After menstruation the basalis regions of the uterine glands alone persist. The columnar cells evacuate most of their granular contents, and the Golgi apparatus loosens out and breaks up. Text-fig. 3 A.) 2. The postmenstrual cells are cubical limpid cells, with a Golgi apparatus consisting of a few granules arranged in a line. Text-fig. 3 B.) 3. In the proliferative phase the Golgi apparatus re-forms and grows into a characteristic net. The cells become columnar. No marked aggregation of granules has as yet occurred. 4. In the progravid phase, the cells become cubical, the Golgi apparatus spreads, and a marked aggregation of fat and glycogen appears at the inner pole of each cell. (Text-fig. 3 C.) 5. Should pregnancy supervene, the cells secrete numerous ovoid (proteid) granules at their outer poles (Text-fig. 3 D). These granules are topographically related to the outer edge of the Golgi apparatus. 6. The presence of the granules in any specimen of curetting reveals that it has been recovered from a case of interrupted pregnancy. Exceptions to this might be those which are known to hold for the Ascheim-Zondek pregnancy test. 7. The uterine glands contain only one type of secretory cell which may be ciliated or non-ciliated.

scholarly journals Transformation of Lymphocytes in Cultures of Human Peripheral Blood

Blood ◽  
1963 ◽  
Vol 22 (5) ◽  
pp. 614-629 ◽  
Author(s):  
YASUKAZU TANAKA ◽  
LOIS B. EPSTEIN ◽  
GEORGE BRECHER ◽  
FREDERICK STOHLMAN
Keyword(s):  
Tissue Culture ◽  
Endoplasmic Reticulum ◽  
Golgi Apparatus ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Blood Cultures ◽  
Multivesicular Bodies ◽  
Proliferating Cells ◽  
Tracer Studies ◽  
Small Lymphocyte

Abstract Previous tracer studies have demonstrated the lymphocytic origin of proliferating cells in blood cultures. Detailed morphologic observations are reported on the transformation of small lymphocytes into larger cells capable of division in tissue culture of human peripheral blood. The large cells have ample cytoplasm with multivesicular bodies, well-developed Golgi apparatus, scanty endoplasmic reticulum, ample ribosomes, and variable fat-laden vacuoles as well as peculiar granular inclusions, large and occasionally bizarre nuclei with prominent nucleoli. Under the condition of culture no further differentiation of these cells has occurred. The potentialities of the small lymphocyte are discussed in the light of recent radiobiologic and cytogenetic investigations.

Vesicle transfer of storage proteins to the vacuole: The role of the Golgi apparatus and multivesicular bodies

1998 ◽  
Vol 152 (6) ◽  
pp. 659-667 ◽  
Author(s):  
D.G. Robinson ◽  
Matthias Bäumer ◽  
Giselbert Hinz ◽  
Inge Hohl
Keyword(s):  
Golgi Apparatus ◽  
Storage Proteins ◽  
Multivesicular Bodies
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