scholarly journals A Rapid Method for Localization of Tissue Structures or Lesions for Electron Microscopy

1959 ◽  
Vol 5 (3) ◽  
pp. 508-510 ◽  
Author(s):  
Sergio A. Bencosme ◽  
Robert S. Stone ◽  
Harrison Latta ◽  
Sidney C. Madden
Keyword(s):  
Electron Microscopy ◽  
Rapid Method ◽  
Tissue Structures

scholarly journals A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles

1975 ◽  
Vol 148 (3) ◽  
pp. 533-537 ◽  
Author(s):  
R B Beechey ◽  
S A Hubbard ◽  
P E Linnett ◽  
A D Mitchell ◽  
E A Munn
Keyword(s):  
Electron Microscopy ◽  
Rapid Method ◽  
Adenosine Triphosphatase ◽  
Pure Form ◽  
Heart Mitochondria ◽  
Polyacrylamide Gels ◽  
Submitochondrial Particles ◽  
Bovine Heart

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.

A rapid method of whole cell sample preparation for scanning electron microscopy using neodymium chloride

Micron ◽  
2019 ◽  
Vol 124 ◽  
pp. 102687 ◽  
Author(s):  
Ivan Novikov ◽  
Anastasia Subbot ◽  
Andrey Turenok ◽  
Nikolay Mayanskiy ◽  
Igor Chebotar
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sample Preparation ◽  
Rapid Method ◽  
Whole Cell ◽  
Neodymium Chloride ◽  
Cell Sample ◽  
Scanning Electron

scholarly journals A rapid method for embedding tissues for electron microscopy using 1,4-dioxane and Polybed 812.

1980 ◽  
Vol 28 (5) ◽  
pp. 465-467 ◽  
Author(s):  
T P Shearer ◽  
L G Hunsicker
Keyword(s):  
Electron Microscopy ◽  
Rapid Method ◽  
Cellular Structures ◽  
Embedding Medium

A rapid method for embedding tissues for electron microscopy is described. This method, which can be completed within 5 hr, uses 1,4-dioxane as the final dehydrating agent and Polybed 812 as the embedding medium. Satisfactory preservation of cellular structures is consistently achieved with a variety of normal and diseased tissues. This method may be of particular value to diagnostic electron microscopy laboratories where time and simplicity are critical.

scholarly journals A sensitive method for measuring polymerized and depolymerized forms of tubulin in tissues.

1977 ◽  
Vol 74 (2) ◽  
pp. 341-350 ◽  
Author(s):  
D G Pipeleers ◽  
M A Pipeleers-Marichal ◽  
P Sherline ◽  
D M Kipnis
Keyword(s):  
Electron Microscopy ◽  
Rat Brain ◽  
Rapid Method ◽  
Binding Assay ◽  
Fed State ◽  
Stabilizing Solution ◽  
Rat Liver Cells ◽  
Wet Weight ◽  
Brain Tubulin ◽  
Sensitive Method

A rapid method for measuring polymerized and depolymerized forms of tubulin in tissues has been developed. The procedure consists of homogenization and centrifugation of the tissue in a microtubule-stabilizing solution and depolymerization of the precipitated microtubules; polymerized and depolymerized forms of tubulin are quantitated by a colchicine-binding assay. The validity of the technique was assessed by electron microscopy and recovery studies with labeled and unlabeled preparations of polymerized and depolymerized forms of rat brain tubulin. The sensitivity of this technique allows quantitation of tubulin in 150 micrograms of tissue, wet weight. The method demonstrated that both the polymerized and depolymerized forms of tubulin were present in rat liver cells, and that in the fed state 31.3 +/-0.7% of the total tubulin pool was in the polymerized form.

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