scholarly journals STUDIES ON ISOLATED AGGREGATING OLIGORIBONUCLEOPROTEINS OF THE EPIDERMIS WITH HISTOCHEMICAL AND MORPHOLOGICAL CHARACTERISTICS OF KERATOHYALIN

1971 ◽  
Vol 49 (2) ◽  
pp. 405-422 ◽  
Author(s):  
Arthur R. Ugel
Keyword(s):  
Acridine Orange ◽  
Toluidine Blue ◽  
Morphological Characteristics ◽  
Ultrastructural Level ◽  
Methyl Green ◽  
Polyacrylamide Gels ◽  
Ultrastructural Studies ◽  
Sodium Decylsulfate ◽  
Oligomeric Series

Histochemical and ultrastructural studies demonstrate that keratohyalin can be mobilized from fresh specimens of cattle hoof epidermis by 1.0 M potassium phosphate buffer (pH 7.0). Macroaggregates with histochemical characteristics identical to those of in situ keratohyalin granules (staining by Harris' hematoxylin, Congo red, diazotized sulfanilic acid, sodium alizarin sulfonate, toluidine blue, methyl green-pyronin, and acridine orange) and with similar morphological characteristics at the ultrastructural level are formed upon dialyzing the extracted keratohyalin against distilled water. Staining by basic dyes (toluidine blue, methyl green-pyronin, and acridine orange) is abolished by treating either in situ keratohyalin granules or isolated macroaggregates with ribonuclease. Electrophoresis of isolated macroaggregates on polyacrylamide gels in the presence of sodium decylsulfate results in the fractionation of a 13 member oligomeric series of ribonucleoproteins and two non-homologous species of ribonucleoproteins. The oligomeric series can be purified by isolating "stacked" oligomers on low concentration (3%) polyacrylamide gels. Fractionated oligomers on polyacrylamide gels and aggregates formed from purified ribonucleoproteins demonstrate histochemical characteristics identical to those of in situ keratohyalin granules. Aggregates formed from denatured ribonucleoproteins are highly disordered and are markedly different from in situ keratohyalin granules or nondenatured isolated macroaggregates at the ultrastructural level, possibly due to irreversible denaturation of the oligomers by sodium decylsulfate.

scholarly journals CYTOLOGICAL AND CYTOCHEMICAL STUDIES OF GREEN MONKEY KIDNEY CELLS INFECTED IN VITRO WITH SIMIAN VIRUS 40

1965 ◽  
Vol 25 (3) ◽  
pp. 529-543 ◽  
Author(s):  
Robert Love ◽  
Mario V. Fernandes
Keyword(s):  
Nucleic Acids ◽  
Acridine Orange ◽  
Toluidine Blue ◽  
Trichloroacetic Acid ◽  
Simian Virus 40 ◽  
Monkey Kidney ◽  
Methyl Green ◽  
Kidney Cells ◽  
Green Monkey ◽  
Inclusion Material

Cytological and cytochemical studies of green monkey kidney cells infected with SV40 virus indicated that the type of lesion produced was influenced by the multiplicity of infection and that the lesions appeared later and progressed more slowly when the inoculum was diluted. The earliest change consisted of enlargement of ribonucleoprotein-containing spherules in the nucleolus (nucleolini). This was followed by rarefaction, with or without condensation, of the chromatin and the appearance of one or more homogeneous masses of inclusion material containing DNA, RNA, and non-histone protein which eventually filled the nucleus. In some instances the chromatin appeared to be directly transformed into inclusion material. In the later stages of infection, the ribonucleoprotein of the nucleolini was no longer stainable and material resembling the nucleoprotein of the intranuclear inclusions was found in the nucleolar vacuoles and in the cytoplasm. The nucleic acids in the inclusions were stained by toluidine blue, toluidine blue-molybdate, the Feulgen stain, and by methyl green. The stainable material was extractable by nuclease digestion or by hot trichloroacetic acid. Green or yellowish green staining by acridine orange was apparently due to binding of dye by protein and not by nucleic acids since the staining reaction was not reduced by extraction of nucleic acids by hot trichloroacetic acid. Extraction with pepsin in combination with ribonuclease or deoxyribonuclease removed practically all the inclusions from the cells; consequently they could not be stained with acridine orange. The cytochemical studies suggest that the use of pepsin together with nuclease is not a meaningful technique.

Immunocytochemistry. A Review of Methodology for Electron Microscopic Applicaiton

1974 ◽  
Vol 32 ◽  
pp. 328-329
Author(s):  
Joseph E. Mazurkiewicz
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Ultrastructural Level ◽  
Electron Microscopic ◽  
Biological Interest ◽  
Investigative Approach ◽  
Immunologic Activity ◽  
Dense Label

Immunocytochemistry is a powerful investigative approach in which one of the most exacting examples of specificity, that of the reaction of an antibody with its antigen, isused to localize tissue and cell specific molecules in situ. Following the introduction of fluorescent labeled antibodies in T950, a large number of molecules of biological interest had been studied with light microscopy, especially antigens involved in the pathogenesis of some diseases. However, with advances in electron microscopy, newer methods were needed which could reveal these reactions at the ultrastructural level. An electron dense label that could be coupled to an antibody without the loss of immunologic activity was desired.

Histochemical and Ultrastructural Studies of the Digestive Epithelium in a Gastropod Gametolytic Organ

1980 ◽  
Vol 38 ◽  
pp. 568-569
Author(s):  
R. L. Reeder ◽  
S. H. Rogers ◽  
W. A. Shannon
Keyword(s):  
Acid Phosphatase ◽  
Genital Tract ◽  
Reproductive Tract ◽  
Ultrastructural Level ◽  
Conclusive Evidence ◽  
Digestive Organ ◽  
Limited Information ◽  
Ultrastructural Studies ◽  
Morphological Studies

Numerous morphological studies have dealt with the spermatheca of pulmonate gastropods. This globular organ, which is attached to the female portion of the reproductive tract by a long duct in these monoecious animals, has had various functions ascribed to it. Recent histochemical demonstrations of deoxyribonuclease, ribonuclease, protease, and acid phosphatase have provided, however, conclusive evidence that it is a digestive organ for the degradation of superfluous sperm and genital tract secretions. Only limited information concerning the spermatheca is available at the ultrastructural level, a fact providing the stimulus for the present study of this organ in Sonorella santaritana, a desert mountain snail from Arizona.

scholarly journals Ultrastructural Studies of Human Basophils and Mast Cells

2005 ◽  
Vol 53 (9) ◽  
pp. 1043-1070 ◽  
Author(s):  
Ann M. Dvorak
Keyword(s):  
Growth Factor ◽  
Mast Cells ◽  
Cord Blood ◽  
Blood Cells ◽  
De Novo ◽  
Ultrastructural Studies ◽  
Specific Growth Factor ◽  
Cord Blood Cells ◽  
Human Basophils

Ultrastructural studies of human mast cells (HMCs) and basophils (HBs) are reviewed. Sources of HMCs include biopsies of tissue sites and in situ study of excised diseased organs; isolated, partially purified samples from excised organs; and growth-factor-stimulated mast cells that develop de novo in cultures of cord blood cells. Sources of HBs for study include partially purified peripheral blood basophils, basophils in tissue biopsies, and specific growth factor-stimulated basophils arising de novo from cord blood cells. The ultrastructural studies reviewed deal with identity, secretion, vesicles, recovery, and synthesis issues related to the biology of these similar cells.

scholarly journals Radiomorphology of the Habib Sealer-Induced Resection Plane during Long-Time Followup:A Longitudinal Single Center Experience after 64 Radiofrequency-AssistedLiver Resections

HPB Surgery ◽  
2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Robert Kleinert ◽  
Roger Wahba ◽  
Christoph Bangard ◽  
Klaus Prenzel ◽  
Arnulf H. Hölscher ◽  
...  
Keyword(s):  
Liver Resection ◽  
Morphological Characteristics ◽  
Postoperative Mortality ◽  
Coagulation Necrosis ◽  
Time Interval ◽  
Safe Alternative ◽  
Time Points ◽  
Single Center Experience ◽  
Long Time

Background. Radiofrequency (RF-) assisted liver resection devices like the Habib sealer induce a necrotic resection plane from which a small margin of necrotic liver tissue remains in situ. The aim of the present paper was to report our long-time experience with the new resection method and the morphological characteristics of the remaining necrotic resection plane. Methods. 64 RF-assisted liver resections were performed using the Habib sealer. Followup was assessed at defined time points. Results. The postoperative mortality was 3,6% and morbidity was 18%. The followup revealed that the necrotic zone was detectable in all analyzed CT and MRI images as a hypodense structure without any contrast enhancement at all time points, irrespectively of the time interval between resection and examination. Conclusion. Liver resection utilizing radiofrequency-induced resection plane coagulation is a safe alternative to the established resection techniques. The residual zone of coagulation necrosis remains basically unchanged during a followup of three years. This has to be kept in mind when evaluating the follow up imaging of these patients.

Cell cycle redistribution of U3 snRNA and fibrillarin. Presence in the cytoplasmic nucleolus remnant and in the prenucleolar bodies at telophase

1994 ◽  
Vol 107 (2) ◽  
pp. 463-475 ◽  
Author(s):  
M.C. Azum-Gelade ◽  
J. Noaillac-Depeyre ◽  
M. Caizergues-Ferrer ◽  
N. Gas
Keyword(s):  
Cell Cycle ◽  
Immunofluorescence Microscopy ◽  
Close Association ◽  
Ultrastructural Level ◽  
Small Nuclear Rna ◽  
Cho Cell ◽  
Nucleolar Proteins ◽  
Nuclear Rna ◽  
Active Nors

The distribution of the U3 small nuclear RNA during the cell cycle of the CHO cell line was studied by in situ hybridization using digoxigenin-labelled oligonucleotide probes. The location of the hybrids by immunofluorescence microscopy and at the ultrastructural level was correlated with the distribution of two nucleolar proteins, nucleolin and fibrillarin. The U3 snRNA molecules persist throughout mitosis in close association with the nucleolar remnant. U3 snRNA is present in the prenucleolar bodies (PNBs) and could participate in nucleologenesis in association with several nucleolar proteins such as nucleolin and fibrillarin. The interaction of U3 snRNP with the 5′ external spacer of pre-RNA newly synthesized by active NORs is proposed to be the promoting event of nucleologenesis.

scholarly journals A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA.

1993 ◽  
Vol 41 (1) ◽  
pp. 7-12 ◽  
Author(s):  
J H Wijsman ◽  
R R Jonker ◽  
R Keijzer ◽  
C J van de Velde ◽  
C J Cornelisse ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Staining Method ◽  
Morphological Characteristics ◽  
Image Cytometry ◽  
Apoptotic Cells ◽  
Dna Breaks ◽  
Paraffin Sections ◽  
Tissue Sections

Apoptosis (programmed cell death) can be difficult to detect in routine histological sections. Since extensive DNA fragmentation is an important characteristic of this process, visualization of DNA breaks could greatly facilitate the identification of apoptotic cells. We describe a new staining method for formalin-fixed, paraffin-embedded tissue sections that involves an in situ end-labeling (ISEL) procedure. After protease treatment to permeate the tissue sections, biotinylated nucleotides are in situ incorporated into DNA breaks by polymerase and subsequently stained with DAB via peroxidase-conjugated avidin. Staining of cells with the morphological characteristics of apoptosis was demonstrated in tissues known to exhibit programmed cell death, i.e., prostate and uterus after castration, tumors, lymph node follicles, and embryos. Apoptotic cells could be discriminated morphologically from areas of labeled necrotic cells, in which DNA degradation also occurs. Because apoptosis is relatively easily recognized in H&E-stained sections of involuting prostates of castrated rats, we used this model system to validate the ISEL method for the quantification of apoptotic cells. A high correlation was found between the fractions of ISEL-labeled cells and the fractions of apoptotic cells that were morphologically determined in adjacent sections. We conclude that ISEL is a useful technique for quantification of apoptosis in paraffin sections, especially for those tissues in which morphological determination is difficult. Furthermore, this new staining method enables the use of automated image cytometry for evaluating apoptosis.

scholarly journals ELECTROCHEMICAL OXIDATION OF ETHANOL USING Ni-Co-PVC COMPOSITE ELECTRODE

2011 ◽  
Vol 11 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Riyanto Riyanto ◽  
Mohamed Rozali Othman ◽  
Jumat Salimon
Keyword(s):  
Polyvinyl Chloride ◽  
Electrochemical Behavior ◽  
Morphological Characteristics ◽  
Redox System ◽  
Electrochemical Technique ◽  
Metal Foil ◽  
Composite Electrodes ◽  
Nickel Metal ◽  
Nickel Cobalt

The morphological characteristics and electrochemical behavior of nickel metal foil (Ni), nickel-polyvinyl chloride (Ni-PVC) and nickel-cobalt-polyvinyl chloride (Ni-Co-PVC) electrodes in alkaline solution has been investigated. The morphological characteristics of the electrode surface were studied using SEM and EDS, while the electrochemical behavior of the electrodes was studied using cyclic voltammetry (CV). It was found that composite electrodes (Ni-PVC and Ni-Co-PVC) have a porous, irregular and rough surface. In situ studies using electrochemical technique using those three electrodes exhibited electrochemical activity for redox system, as well as selectivity in the electrooxidation of ethanol to acetic acid. The studies also found that an electrokinetics and electrocatalytic activity behaviors of the electrodes prepared were Ni metal foil

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