scholarly journals RNA SYNTHESIS IN CHINESE HAMSTER CELLS

1969 ◽  
Vol 42 (1) ◽  
pp. 308-315 ◽  
Author(s):  
M. D. Enger ◽  
R. A. Tobey
Keyword(s):  
Life Cycle ◽  
Rna Synthesis ◽  
Gene Dosage ◽  
Chinese Hamster ◽  
Suspension Cultures ◽  
Dosage Effect ◽  
Gene Dosage Effect ◽  
Chinese Hamster Cells ◽  
Total Rna ◽  
Mechanical Selection

Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-14C or with uridine-3H at different stages in the life cycle. The rate of 14C incorporation into 18S RNA was measured, as was the rate of uridine-3H incorporation into total RNA for both monolayer and suspension cultures. The rate of incorporation increased continuously throughout interphase in a fashion inconsistent with a gene-dosage effect upon RNA synthesis.

scholarly journals RNA SYNTHESIS IN CHINESE HAMSTER CELLS

1968 ◽  
Vol 36 (3) ◽  
pp. 583-593 ◽  
Author(s):  
M. D. Enger ◽  
R. A. Tobey ◽  
A. G. Saponara
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Specific Activity ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Sedimentation Analysis ◽  
Ovary Cells ◽  
Chinese Hamster Cells ◽  
Mechanical Selection ◽  
Selection Of

The incorporation of methionine-methyl-14C into 18S ribosomal RNA of cultured Chinese hamster ovary cells in early and late interphase has been determined by zone-sedimentation analysis of phenol-extracted RNA preparations. Synchronized cell cultures were prepared for these studies by thymidine treatment and by mechanical selection of mitotic cells. The specific activity of 18S RNA labeled in late interphase was found to be 1.1–1.2 times that of 18S RNA labeled in early interphase. Upon correction for increase in RNA mass, the rate of methylation of 18S RNA in late interphase is about 1.9 times that in early interphase.

scholarly journals REGULATION OF INITIATION OF DNA SYNTHESIS IN CHINESE HAMSTER CELLS

1970 ◽  
Vol 46 (1) ◽  
pp. 151-157 ◽  
Author(s):  
R. A. Tobey ◽  
K. D. Ley
Keyword(s):  
Cell Cycle ◽  
Life Cycle ◽  
Stationary Phase ◽  
Dna Synthesis ◽  
Simple Technique ◽  
Chinese Hamster ◽  
Suspension Cultures ◽  
Chinese Hamster Cells ◽  
Synchronized Cells ◽  
Biochemical Mechanisms

Suspension cultures of Chinese hamster cells (line CHO) were grown to stationary phase (approximately 8–9 x 105 cells/ml) in F-10 medium. Cells remained viable (95%) for at least 80 hr in stationary phase, and essentially all of the cells were in G1 Upon resuspension or dilution with fresh medium, the cells were induced to resume traverse of the life cycle in in synchrony, and the patterns of DNA synthesis and division were similar to those observed in cultures prepared by mitotic selection. Immediately after dilution, the rates of synthesis of RNA and protein increased threefold. This system provides a simple technique for production of large quantities of highly synchronized cells and may ultimately provide information on the biochemical mechanisms regulating cell-cycle traverse.

Evidence of a gene-dosage effect for the glucocorticoid receptor in trisomy 18 mice

1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S95-S96
Author(s):  
D. VOGLIOLO ◽  
H. WINKING ◽  
R. KNUPPEN
Keyword(s):  
Glucocorticoid Receptor ◽  
Gene Dosage ◽  
Dosage Effect ◽  
Trisomy 18 ◽  
Gene Dosage Effect

Apparent Lack of Gene Dosage Effect in the PLT Assay

1977 ◽  
Vol 6 (5) ◽  
pp. 529-532 ◽  
Author(s):  
S. JARAMILLO ◽  
G. ANHORN ◽  
F. SCHUNTER ◽  
P. WERNET
Keyword(s):  
Gene Dosage ◽  
Dosage Effect ◽  
Gene Dosage Effect ◽  
Apparent Lack

Gene dosage effect in expression of blood group antigens

1973 ◽  
Vol 83 (2) ◽  
pp. 167-172
Author(s):  
Li-Tsun Chen ◽  
Joseph A. Davidenas ◽  
Roal F. Ruth
Keyword(s):  
Blood Group ◽  
Gene Dosage ◽  
Dosage Effect ◽  
Blood Group Antigens ◽  
Gene Dosage Effect

scholarly journals THE GENETIC BASIS OF DOSAGE COMPENSATION OF ALCOHOL DEHYDROGENASE-1 IN MAIZE

Genetics ◽  
1981 ◽  
Vol 97 (3-4) ◽  
pp. 625-637 ◽  
Author(s):  
James A Birchler
Keyword(s):  
Alcohol Dehydrogenase ◽  
Structural Gene ◽  
Dosage Compensation ◽  
Genetic Basis ◽  
Gene Dosage ◽  
Dosage Effect ◽  
Gene Dosage Effect ◽  
Negative Effect ◽  
Higher Eukaryotes ◽  
Alcohol Dehydrogenase 1

ABSTRACT The levels of alcohol dehydrogenase (ADH) do not exhibit a structural gene-dosage effect in a one to four dosage series of the long arm of chromosome one (1L) (BIRCHLER19 79). This phenomenon, termed dosage compensation, has been studied in more detail. Experiments are described in which individuals aneuploid for shorter segments were examined for the level of ADH in order to characterize the genetic nature of the compensation. The relative ADH expression in segmental trisomics and tetrasomics of region IL 0.72–0.90, which includes the Adh locus, approaches the level expected from a strict gene dosage effect. Region IL 0.20–0.72 produces a negative effect upon ADH in a similar manner to that observed with other enzyme levels when IL as a whole is varied (BIRCHLEF1I9 79). These and other comparisons have led to the concept that the compensation of ADH results from the cancellation of the structural gene effect by the negative aneuploid effect. The example of ADH is discussed as a model for certain other cases of dosage compensation in higher eukaryotes.

Further evidence for the assignment of the red cell acid phosphatase gene (ACP1) to the short arm of chromosome 2 from gene dosage effect

1976 ◽  
Vol 16 (1-5) ◽  
pp. 326-327
Author(s):  
R.E. Magenis ◽  
R.D. Koler ◽  
E. Lovrien ◽  
R.H. Bigley ◽  
M.C. DuVal ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Gene Dosage ◽  
Dosage Effect ◽  
Red Cell ◽  
Chromosome 2 ◽  
Gene Dosage Effect ◽  
I Gene
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