scholarly journals A STUDY OF NEWT MITOTIC CHROMOSOMES BY NEGATIVE STAINING

1967 ◽  
Vol 32 (3) ◽  
pp. 585-603 ◽  
Author(s):  
N. A. Barnicot
Keyword(s):  
Calcium Chloride ◽  
Chloride Solution ◽  
Heart Tissue ◽  
Uranyl Acetate ◽  
Ammonium Molybdate ◽  
Calcium Chloride Solution ◽  
Mitotic Chromosomes ◽  
Parallel Fibers ◽  
Fluid Surface ◽  
Nodular Structure

A method is described for bursting single, selected mitotic cells on a fluid surface. Cells from cultures of newt heart tissue were burst on dilute solutions containing potassium and sodium with and without added calcium and also on dilute calcium chloride solution. The material was negatively stained with uranyl acetate or sometimes with ammonium molybdate or sodium phosphotungstate. The bodies of chromatids spread on NaCl/KCl solutions showed many parallel fibers about 150 A in diameter. Loops with a complex nodular structure were observed projecting from the sides and ends of chromatids. In calcium-containing solutions there was evidence of fiber coagulation; the chromatid body was more compact and laterally projecting fibers tended to be pulled out straight. Especially in the absence of calcium the chromosomal fibers had a nodular form and appeared to be composed of irregularly folded fibrillar elements. The question as to whether chromosomal fibers, which range in diameter from about 50 to 300 A, consist of single, folded threads or of two or more adjacent subunits is discussed.

scholarly journals A Note on the Structure of Spindle Fibres

1966 ◽  
Vol 1 (2) ◽  
pp. 217-222
Author(s):  
N. A. BARNICOT
Keyword(s):  
Chloride Solution ◽  
Heart Tissue ◽  
Uranyl Acetate ◽  
Ammonium Molybdate ◽  
Calcium Chloride Solution ◽  
Electron Microscopic ◽  
Microscopic Appearance ◽  
Human Fibroblast Cultures ◽  
Electron Microscopic Appearance ◽  
Spindle Fibres

Single mitotic cells from cultures of newt heart tissue or from human fibroblast cultures were burst on a dilute calcium chloride solution and negatively stained on electron-microscope grids. The electron-microscopic appearance of spindle fibres negatively stained with uranyl acetate and with ammonium molybdate is described. As in the case of fibres from sperm tails, the spindle fibres were found to be tubular and the wall was seen to be composed of longitudinal fibrils about 35 Å in diameter.

POLARIMETRIC DETERMINATION OF STARCH IN CEREAL PRODUCTS: IV. CRITICAL STUDIES OF METHODS FOR THE DETERMINATION OF STARCH IN WHOLE WHEAT, GRANULAR, AND PATENT FLOURS

1945 ◽  
Vol 23b (6) ◽  
pp. 239-259 ◽  
Author(s):  
K. A. Clendenning
Keyword(s):  
Calcium Chloride ◽  
Chloride Solution ◽  
Specific Rotation ◽  
Large Error ◽  
Uranyl Acetate ◽  
Calcium Chloride Solution ◽  
Stannic Chloride ◽  
Rice Grain ◽  
Whole Wheat

A rapid polarimetric method applicable to whole wheat, granular, and patent flour is described in which the starch is dissolved in hot calcium chloride solution under uniform conditions, soluble proteins being removed by the addition of stannic chloride or uranyl acetate prior to volume adjustment, filtration, and polarization.Approximately 2.5-gm. samples (40 mesh) are placed in tall beakers and suspended uniformly in 10 cc. of water. Calcium chloride solution (60 cc., d. 1.30, pH 5.5) and 2 cc. of 0.8% acetic acid are added. The mixture is boiled at constant salt concentration for 15 min., foaming being prevented by dropwise addition of n-octanol as required. After cooling, 2.5 to 5.0 cc. of 4% stannic chloride or 10 cc. of 5% uranyl acetate (each dissolved in calcium chloride solution) is added, the mixture is diluted to 100 cc. with calcium chloride solution, mixed, and filtered. After discarding the first portion, the filtrate is polarized in 2 dm. tubes. [α]D = + 203° is used in the calculations; % starch = 2 dm. reading in degrees × 10 when 2.463 gm. flour samples are employed.Stannic chloride is shown to depress the specific rotation value of starches. A large error is introduced when it is used on wheat flour in the amount prescribed by Mannich and Lenz. Using the above uranyl acetate treatment, starch may be rapidly determined in corn, barley, rye, rice, grain sorghum, and buckwheat by the calcium chloride polarimetric method. It is shown that interfering wheat proteins may be removed by preparatory extraction with aqueous ethanol and dilute sodium hydroxide, as well as by precipitation. The latter being more rapid is judged most suitable for routine applications. Levorotatory hemicelluloses of bran and endosperm are dissolved in considerable amount by boiling calcium chloride solution but their small effect is balanced by the error of overestimation arising from insoluble wheat solids. Hemicelluloses are shown to be capable of causing high results in applications of the diastase–hydrochloric-acid method to wheat products. The improved calcium chloride polarimetric method is considered the most reliable of existing macro-methods for the determination of starch in wheat.

Treatment of sepsis with calcium salts. Professor Petrov (V. Chir. and Pogr. Obl., Vol. I, No. 2), Chistyakov (Nov. Chir. Arch., Vol. II, No. 1)

1923 ◽  
Vol 19 (2) ◽  
pp. 100-100
Author(s):  
V. Bogolyubov
Keyword(s):  
Calcium Chloride ◽  
Chloride Solution ◽  
Calcium Chloride Solution ◽  
Calcium Salts ◽  
Intravenous Infusions ◽  
Surgical Methods

The author warmly recommends treatment of sepsis with intravenous infusions of 1% calcium chloride solution (the method first proposed by Prof. Aleksinski). Although this method does not seem sufficiently theoretically justified, nevertheless P., on the basis of his observations, advises to resort to intravenous infusions of 1% solution of crystalline calcium chloride at 250-400 cfu for acute and subacute forms of sepsis where the well-known surgical methods of dissection and removal of infected nidi have been carried to completion and have not put the patient on the path to recovery.

Studying on Preperation and Emulsifying Properties of Silk Protein Calcium Salt Made from Waste Silk

2011 ◽  
Vol 197-198 ◽  
pp. 60-64 ◽  
Author(s):  
Jun Sheng Li ◽  
Hai Tao Cheng
Keyword(s):  
Aqueous Solution ◽  
Calcium Chloride ◽  
Salt Solution ◽  
Chloride Solution ◽  
Calcium Salt ◽  
Calcium Chloride Solution ◽  
Silk Protein ◽  
Emulsifying Properties ◽  
Emulsification Capacity ◽  
New Protein

One key step for silk protein further uses is to let them be dissolved in aqueous solution. However, the silk protein is usually not soluble in aqueous solution. Silk protein dissolved in calcium chloride solution is one of the few effective ways. Silk fibroin was well dissolved in 15-20min. in boiling calcium chloride solution [50% (w/v)] with the ratio of 15g dry waste silk per100mL calcium chloride solution. After dialysis, silk protein calcium salt unexpectedly showed excellent emulsification capacity and stability. Only 0.8-1.2% of silk protein calcium salt solution (50mL) could let 50mL soybean salad oil be mixed and emulsified well. Silk protein calcium salt may be used as a new protein-based surfactant.

Longitudinal distribution of applied calcium, and of naturally occurring calcium, magnesium, and potassium, in Merton apple fruits

1973 ◽  
Vol 24 (3) ◽  
pp. 363 ◽  
Author(s):  
TL Lewis ◽  
D Martin
Keyword(s):  
Calcium Chloride ◽  
Chloride Solution ◽  
Relative Effectiveness ◽  
Calcium Chloride Solution ◽  
Magnesium Concentration ◽  
Longitudinal Distribution ◽  
Time Of Application ◽  
Naturally Occurring ◽  
Bitter Pit ◽  
Apple Fruits

When 45Ca-labelled calcium chloride solution was applied to the skin of young developing Merton apple fruits, activity in the cortex at maturity was highest in the calyx end region where bitter pit lesions most commonly develop. Fruit age at time of application affected the amount of labelled calcium absorbed but not its longitudinal distribution. Following branch injection of labelled calcium chloride solution 8 weeks before harvest, activity in mature fruits was found mostly in the stem end. Leaves and buds accounted for 95% of the recovered activity, and the fruits for 5%, of which about one-quarter was in the calyx half. The same distribution pattern was found following injection of labelled calcium chloride solution into the fruit stem. The concentration of naturally occurring calcium in the cortex of the mature fruit declined steadily from stem end to calyx end. On the other hand, the magnesium concentration was lowest near the stem end and highest at the calyx end. The potassium concentration remained fairly constant along most of the length of the fruit, with a small increase at each end. The findings are discussed with regard to the longitudinal gradient in bitter pit susceptibility within apple fruits, and to the relative effectiveness of tree sprays of calcium, as compared with soil applications, in the control of the disorder. Results suggest that calcium does not have a trace element role in the control of bitter pit.

Effect of immersion time of restorative glass ionomer cements and immersion duration in calcium chloride solution on surface hardness

2014 ◽  
Vol 30 (12) ◽  
pp. e377-e383 ◽  
Author(s):  
Maho Shiozawa ◽  
Hidekazu Takahashi ◽  
Naohiko Iwasaki ◽  
Takahiro Wada ◽  
Motohiro Uo
Keyword(s):  
Calcium Chloride ◽  
Chloride Solution ◽  
Immersion Time ◽  
Surface Hardness ◽  
Calcium Chloride Solution ◽  
Glass Ionomer Cements ◽  
Glass Ionomer

Treatment of acute gonorrheal adnexitis

1927 ◽  
Vol 23 (5) ◽  
pp. 593-593
Keyword(s):  
Calcium Chloride ◽  
Chloride Solution ◽  
Calcium Chloride Solution ◽  
Intravenous Injections ◽  
Anti Inflammatory

The author recommends for this purpose intravenous injections of afenil (10% calcium chloride solution by Knoll) in a dose of 10 cc. Performing these injections once a day, it is possible, in acute and subacute gonorrhoids salpingo-oophoritis, in 8 - 12 days to obtain such results, such with conventional anti-inflammatory treatment are obtained only in 3-4 weeks, and doing injections on 2 times a day, it is possible already in 2-3 days to stop "acute adnexitis.

Intravenous infusions of calcium chloride in gonorrhoeal epididymitis and arthritis

1927 ◽  
Vol 23 (2) ◽  
pp. 252-253
Author(s):  
Ya. D. Pechnikov
Keyword(s):  
Calcium Chloride ◽  
Chloride Solution ◽  
Calcium Chloride Solution ◽  
Intravenous Infusions

The authors used an intravenous 2% calcium chloride solution in an amount of 25 cbc for gonorrhoeal epididymitis and arthritis.

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