scholarly journals THE SYNTHESIS OF DNA, RNA, AND NUCLEAR PROTEIN IN NORMAL AND TUMOR STRAIN CELLS

1966 ◽  
Vol 28 (2) ◽  
pp. 257-261 ◽  
Author(s):  
John Seed
Keyword(s):  
Dna Synthesis ◽  
Nuclear Protein ◽  
Ascites Tumor ◽  
Significant Extent ◽  
Physical Measurements ◽  
Nuclear Rna ◽  
X Irradiation ◽  
Mouse Ascites ◽  
Cell Strains ◽  
Photometric Measurements

Interferometric and photometric measurements have been made on replicating mouse ascites tumor cell cultures. From a study of the relations between successive physical measurements on individual cells, it was found that whereas the net syntheses of nuclear RNA and nuclear protein are closely associated during interphase, they are dissociated from DNA replication to a significant extent. These results agree with others reported in replicating cell strains derived from tumors. In auxiliary experiments an attempt was made to block the initiation of DNA synthesis by X-irradiation: although large amounts of nuclear protein accumulated in some cells in the absence of DNA synthesis, the inability to hold the DNA block for an interphase time prevented a quantitative analysis of the results.

scholarly journals THE SYNTHESIS OF DNA, RNA, AND NUCLEAR PROTEIN IN NORMAL AND TUMOR STRAIN CELLS

1966 ◽  
Vol 28 (2) ◽  
pp. 249-256 ◽  
Author(s):  
John Seed
Keyword(s):  
Dna Synthesis ◽  
Cell Cultures ◽  
Nuclear Protein ◽  
Mouse Cell ◽  
X Rays ◽  
Normal Tissues ◽  
Physical Measurements ◽  
Cytoplasmic Rna ◽  
Nuclear Rna ◽  
Photometric Measurements

Interferometric and photometric measurements have been made on replicating embryo mouse cell cultures. From a study of the relations between successive physical measurements on individual cells, it was found that the net syntheses of DNA, nuclear RNA, nuclear protein, and cytoplasmic RNA are closely associated during interphase. In auxiliary experiments, an inhibition of the onset of DNA synthesis (produced by a dose of X-rays) was found to block the majority of the accumulation of nuclear protein and nuclear RNA. These results are consistent with others previously reported in dividing cell cultures freshly prepared from normal tissues.

scholarly journals THE SYNTHESIS OF DNA, RNA, AND NUCLEAR PROTEIN IN NORMAL AND TUMOR STRAIN CELLS

1966 ◽  
Vol 28 (2) ◽  
pp. 233-248 ◽  
Author(s):  
John Seed
Keyword(s):  
Cell Cultures ◽  
Nuclear Protein ◽  
Kidney Cells ◽  
X Rays ◽  
Normal Tissues ◽  
Physical Measurements ◽  
Cytoplasmic Rna ◽  
Human Cell Cultures ◽  
Nuclear Rna ◽  
Photometric Measurements

Interferometric and photometric measurements have been made on replicating embryo human cell cultures. From a study of the relations between successive physical measurements on individual cells, it was found that the net syntheses of DNA, nuclear RNA, nuclear protein, and cytoplasmic RNA are closely associated during interphase. Additional measurements of DNA and cytoplasmic RNA on freshly prepared replicating monkey kidney cells gave similar results. In auxiliary experiments with embryo human cells, an inhibition of the onset of DNA synthesis (produced by a dose of X-rays) was found to block the majority of the accumulation of nuclear protein and RNA and about half the accumulation of cytoplasmic RNA. These results are consistent with others previously reported in dividing cell cultures freshly prepared from normal tissues.

scholarly journals THE SYNTHESIS OF DNA, RNA, AND NUCLEAR PROTEIN IN NORMAL AND TUMOR STRAIN CELLS

1966 ◽  
Vol 28 (2) ◽  
pp. 263-275 ◽  
Author(s):  
John Seed
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Hela Cells ◽  
Nuclear Protein ◽  
X Rays ◽  
Normal Cells ◽  
Human Carcinoma ◽  
Physical Measurements ◽  
Cytoplasmic Rna ◽  
Nuclear Rna

Interferometric and photometric measurements have been made on HeLa cells, a strain of cells originally derived from a human carcinoma. From a study of the relations between successive physical measurements on individual cells, it was confirmed that, whereas the net syntheses of nuclear RNA and nuclear protein are closely associated during interphase, they are dissociated from DNA replication to a significant extent. These results on nuclear metabolism agree with others previously reported in cell strains derived from tumors; they contrast with results from freshly prepared normal cells, where the net syntheses of DNA, nuclear RNA, and protein are closely associated during interphase. Cytoplasmic measurements on HeLa cells showed that much of the net synthesis of cytoplasmic RNA is associated with DNA replication as in normal cells, and they failed to detect transfer from the nucleus of a stable RNA component synthesized independently from DNA replication. In auxiliary experiments, an inhibition of the onset of DNA synthesis was produced by a dose of X-rays; under these conditions it was shown that the major part of the accumulation of nuclear protein was independent of DNA replication and that the accumulation of nuclear RNA was equivalent to or slightly less than that of nuclear protein. About half the accumulation of cytoplasmic RNA was inhibited when DNA synthesis was blocked.

Action of cell extract of Ehrlich's mouse ascites tumor on mitotic activity and DNA synthesis in that tumor

1977 ◽  
Vol 84 (1) ◽  
pp. 1022-1025
Author(s):  
A. I. Antokhin ◽  
V. P. Barysheva ◽  
Yu. A. Romanov ◽  
V. P. Rybakov
Keyword(s):  
Dna Synthesis ◽  
Mitotic Activity ◽  
Cell Extract ◽  
Ascites Tumor ◽  
Mouse Ascites
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