Action of cell extract of Ehrlich's mouse ascites tumor on mitotic activity and DNA synthesis in that tumor

1977 ◽  
Vol 84 (1) ◽  
pp. 1022-1025
Author(s):  
A. I. Antokhin ◽  
V. P. Barysheva ◽  
Yu. A. Romanov ◽  
V. P. Rybakov
Keyword(s):  
Dna Synthesis ◽  
Mitotic Activity ◽  
Cell Extract ◽  
Ascites Tumor ◽  
Mouse Ascites

scholarly journals THE SYNTHESIS OF DNA, RNA, AND NUCLEAR PROTEIN IN NORMAL AND TUMOR STRAIN CELLS

1966 ◽  
Vol 28 (2) ◽  
pp. 257-261 ◽  
Author(s):  
John Seed
Keyword(s):  
Dna Synthesis ◽  
Nuclear Protein ◽  
Ascites Tumor ◽  
Significant Extent ◽  
Physical Measurements ◽  
Nuclear Rna ◽  
X Irradiation ◽  
Mouse Ascites ◽  
Cell Strains ◽  
Photometric Measurements

Interferometric and photometric measurements have been made on replicating mouse ascites tumor cell cultures. From a study of the relations between successive physical measurements on individual cells, it was found that whereas the net syntheses of nuclear RNA and nuclear protein are closely associated during interphase, they are dissociated from DNA replication to a significant extent. These results agree with others reported in replicating cell strains derived from tumors. In auxiliary experiments an attempt was made to block the initiation of DNA synthesis by X-irradiation: although large amounts of nuclear protein accumulated in some cells in the absence of DNA synthesis, the inability to hold the DNA block for an interphase time prevented a quantitative analysis of the results.

DNA synthesis and mitotic activity during early development of chick lens

1968 ◽  
Vol 17 (5) ◽  
pp. 544-561 ◽  
Author(s):  
Sohan P. Modak ◽  
Georgia Morris ◽  
Tuneo Yamada
Keyword(s):  
Dna Synthesis ◽  
Mitotic Activity ◽  
Early Development ◽  
Chick Lens

Replication of cloned DNA containing the Alu family sequence during cell extract-promoting simian virus 40 DNA synthesis

1984 ◽  
Vol 4 (8) ◽  
pp. 1476-1482
Author(s):  
H Ariga
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Rna Polymerase Iii ◽  
Simian Virus 40 ◽  
Cell Extract ◽  
Simian Virus ◽  
T Antigen ◽  
Sv40 T Antigen ◽  
Sv40 Dna

The replicating activity of several cloned DNAs containing putative origin sequences was examined in a cell-free extract that absolutely depends on simian virus 40 (SV40) T antigen promoting initiation of SV40 DNA replication in vitro. Of the three DNAs containing the human Alu family sequence (BLUR8), the origin of (Saccharomyces cerevisiae plasmid 2 micron DNA (pJD29), and the yeast autonomous replicating sequence (YRp7), only BLUR8 was active as a template. Replication in a reaction mixture with BLUR8 as a template was semiconservative and not primed by a putative RNA polymerase III transcript synthesized on the Alu family sequence in vitro. Pulse-chase experiments showed that the small-sized DNA produced in a short-term incubation was converted to full-length closed circular and open circular DNAs in alkaline sucrose gradients. DNA synthesis in extracts began in a region of the Alu family sequence and was inhibited 80% by the addition of anti-T serum. Furthermore, partially purified T antigen bound the Alu family sequence in BLUR8 by the DNA-binding immunoassay. These results suggest that SV40 T antigen recognizes the Alu family sequence, similar to the origin sequence of SV40 DNA, and initiates semiconservative DNA replication in vitro.

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