scholarly journals Hedgehog signaling establishes precursors for germline stem cell niches by regulating cell adhesion

2017 ◽  
Vol 216 (5) ◽  
pp. 1439-1453 ◽  
Author(s):  
Chun-Ming Lai ◽  
Kun-Yang Lin ◽  
Shih-Han Kao ◽  
Yi-Ning Chen ◽  
Fu Huang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Hedgehog Signaling ◽  
Control Cell ◽  
Germline Stem Cell ◽  
Traffic Jam ◽  
Stem Cell Maintenance ◽  
Cell Affinity ◽  
Hh Signaling ◽  
Cell Maintenance

Stem cells require different types of supporting cells, or niches, to control stem cell maintenance and differentiation. However, little is known about how those niches are formed. We report that in the development of the Drosophila melanogaster ovary, the Hedgehog (Hh) gradient sets differential cell affinity for somatic gonadal precursors to specify stromal intermingled cells, which contributes to both germline stem cell maintenance and differentiation niches in the adult. We also report that Traffic Jam (an orthologue of a large Maf transcription factor in mammals) is a novel transcriptional target of Hh signaling to control cell–cell adhesion by negative regulation of E-cadherin expression. Our results demonstrate the role of Hh signaling in niche establishment by segregating somatic cell lineages for differentiation.

scholarly journals The actin-binding protein profilin is required for germline stem cell maintenance and germ cell enclosure by somatic cyst cells

Development ◽  
2013 ◽  
Vol 141 (1) ◽  
pp. 73-82 ◽  
Author(s):  
A. R. Shields ◽  
A. C. Spence ◽  
Y. M. Yamashita ◽  
E. L. Davies ◽  
M. T. Fuller
Keyword(s):  
Stem Cell ◽  
Germ Cell ◽  
Binding Protein ◽  
Actin Binding ◽  
Germline Stem Cell ◽  
Actin Binding Protein ◽  
Stem Cell Maintenance ◽  
Cyst Cells ◽  
Cell Maintenance

scholarly journals Drosophila small ovary gene is required for transposon silencing and heterochromatin organization, and ensures germline stem cell maintenance and differentiation

Development ◽  
2018 ◽  
Vol 145 (23) ◽  
pp. dev170639 ◽  
Author(s):  
Ferenc Jankovics ◽  
Melinda Bence ◽  
Rita Sinka ◽  
Anikó Faragó ◽  
László Bodai ◽  
...  
Keyword(s):  
Stem Cell ◽  
Germline Stem Cell ◽  
Stem Cell Maintenance ◽  
Transposon Silencing ◽  
Small Ovary ◽  
Cell Maintenance

scholarly journals RNAi-based screens uncover a potential new role for the orphan neuropeptide receptor Moody in Drosophila female germline stem cell maintenance

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243756
Author(s):  
Tianlu Ma ◽  
Shinya Matsuoka ◽  
Daniela Drummond-Barbosa
Keyword(s):  
Stem Cell ◽  
Egg Production ◽  
Large Scale ◽  
Signal To Noise Ratio ◽  
Egg Laying ◽  
Melatonin Receptors ◽  
Germline Stem Cell ◽  
Stem Cell Maintenance ◽  
Female Germline ◽  
Cell Maintenance

Reproduction is highly sensitive to changes in physiology and the external environment. Neuropeptides are evolutionarily conserved signaling molecules that regulate multiple physiological processes. However, the potential reproductive roles of many neuropeptide signaling pathways remain underexplored. Here, we describe the results of RNAi-based screens in Drosophila melanogaster to identify neuropeptides/neuropeptide receptors with potential roles in oogenesis. The screen read-outs were either the number of eggs laid per female per day over time or fluorescence microscopy analysis of dissected ovaries. We found that the orphan neuropeptide receptor encoded by moody (homologous to mammalian melatonin receptors) is likely required in somatic cells for normal egg production and proper germline stem cell maintenance. However, the egg laying screens had low signal-to-noise ratio and did not lead to the identification of additional candidates. Thus, although egg count assays might be useful for large-scale screens to identify oogenesis regulators that result in dramatic changes in oogenesis, more labor-intensive microscopy-based screen are better applicable for identifying new physiological regulators of oogenesis with more subtle phenotypes.

scholarly journals Histone H3K9 Trimethylase Eggless Controls Germline Stem Cell Maintenance and Differentiation

PLoS Genetics ◽  
2011 ◽  
Vol 7 (12) ◽  
pp. e1002426 ◽  
Author(s):  
Xiaoxi Wang ◽  
Lei Pan ◽  
Su Wang ◽  
Jian Zhou ◽  
William McDowell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Germline Stem Cell ◽  
Stem Cell Maintenance ◽  
Cell Maintenance
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