scholarly journals Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59fyn and to enhance neurite outgrowth

2005 ◽  
Vol 169 (2) ◽  
pp. 341-354 ◽  
Author(s):  
Antonella Santuccione ◽  
Vladimir Sytnyk ◽  
Iryna Leshchyns'ka ◽  
Melitta Schachner
Keyword(s):  
Neurite Outgrowth ◽  
Lipid Rafts ◽  
Prion Protein ◽  
System Development ◽  
Neuronal Cell ◽  
Neural Cell ◽  
Nervous System Development ◽  
Cellular Prion Protein ◽  
Promote Neurite Outgrowth ◽  
Cis And Trans

In spite of advances in understanding the role of the cellular prion protein (PrP) in neural cell interactions, the mechanisms of PrP function remain poorly characterized. We show that PrP interacts directly with the neural cell adhesion molecule (NCAM) and associates with NCAM at the neuronal cell surface. Both cis and trans interactions between NCAM at the neuronal surface and PrP promote recruitment of NCAM to lipid rafts and thereby regulate activation of fyn kinase, an enzyme involved in NCAM-mediated signaling. Cis and trans interactions between NCAM and PrP promote neurite outgrowth. When these interactions are disrupted in NCAM-deficient and PrP-deficient neurons or by PrP antibodies, NCAM/PrP-dependent neurite outgrowth is arrested, indicating that PrP is involved in nervous system development cooperating with NCAM as a signaling receptor.

scholarly journals Cellular prion protein is essential for oligomeric amyloid- -induced neuronal cell death

2011 ◽  
Vol 21 (5) ◽  
pp. 1138-1144 ◽  
Author(s):  
W. Kudo ◽  
H.-P. Lee ◽  
W.-Q. Zou ◽  
X. Wang ◽  
G. Perry ◽  
...  
Keyword(s):  
Cell Death ◽  
Prion Protein ◽  
Neuronal Cell Death ◽  
Neuronal Cell ◽  
Cellular Prion Protein

Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein

2001 ◽  
Vol 314 (5) ◽  
pp. 1209-1225 ◽  
Author(s):  
Gerold Schmitt-Ulms ◽  
Giuseppe Legname ◽  
Michael A Baldwin ◽  
Haydn L Ball ◽  
Nicole Bradon ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Prion Protein ◽  
Cell Adhesion Molecules ◽  
Neural Cell ◽  
Cellular Prion Protein ◽  
Neural Cell Adhesion Molecules ◽  
Neural Cell Adhesion

scholarly journals Cellular Form of Prion Protein Inhibits Reelin-Mediated Shedding of Caspr from the Neuronal Cell Surface to Potentiate Caspr-Mediated Inhibition of Neurite Outgrowth

2010 ◽  
Vol 30 (27) ◽  
pp. 9292-9305 ◽  
Author(s):  
V. Devanathan ◽  
I. Jakovcevski ◽  
A. Santuccione ◽  
S. Li ◽  
H. J. Lee ◽  
...  
Keyword(s):  
Cell Surface ◽  
Neurite Outgrowth ◽  
Prion Protein ◽  
Neuronal Cell

scholarly journals Cellular Prion Protein and Caveolin-1 Interaction in a Neuronal Cell Line Precedes Fyn/Erk 1/2 Signal Transduction

2006 ◽  
Vol 2006 ◽  
pp. 1-13 ◽  
Author(s):  
Mattia Toni ◽  
Enzo Spisni ◽  
Cristiana Griffoni ◽  
Spartaco Santi ◽  
Massimo Riccio ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Cellular Prion Protein ◽  
Glutathione S Transferase ◽  
Neuronal Cell Line ◽  
Caveolin 1 ◽  
Fyn Kinase

It has been reported that cellular prion protein (PrPc) is enriched in caveolae or caveolae-like domains with caveolin-1 (Cav-1) participating to signal transduction events by Fyn kinase recruitment. By using the Glutathione-S-transferase (GST)-fusion proteins assay, we observed that PrPc strongly interacts in vitro with Cav-1. Thus, we ascertained the PrPc caveolar localization in a hypothalamic neuronal cell line (GN11), by confocal microscopy analysis, flotation on density gradient, and coimmunoprecipitation experiments. Following the anti-PrPc antibody-mediated stimulation of live GN11 cells, we observed that PrPc clustered on plasma membrane domains rich in Cav-1 in which Fyn kinase converged to be activated. After these events, a signaling cascade through p42/44 MAP kinase (Erk 1/2) was triggered, suggesting that following translocations from rafts to caveolae or caveolae-like domains PrPc could interact with Cav-1 and induce signal transduction events.

Prox1 inhibits neurite outgrowth during central nervous system development

2020 ◽  
Author(s):  
Valeria Kaltezioti ◽  
Iosifina P. Foskolou ◽  
Matthieu D. Lavigne ◽  
Elpinickie Ninou ◽  
Matina Tsampoula ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Neurite Outgrowth ◽  
System Development ◽  
Nervous System Development ◽  
Central Nervous System Development

Changes in cellular prion protein expression, processing and localisation during differentiation of the neuronal cell line CAD 5

2019 ◽  
Vol 112 (1) ◽  
pp. 1-21 ◽  
Author(s):  
Zuzana Fremuntova ◽  
Tibor Mosko ◽  
Jakub Soukup ◽  
Johanka Kucerova ◽  
Marie Kostelanska ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Cellular Prion Protein ◽  
Neuronal Cell Line ◽  
Expression Processing
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