Changes in cellular prion protein expression, processing and localisation during differentiation of the neuronal cell line CAD 5

2019 ◽  
Vol 112 (1) ◽  
pp. 1-21 ◽  
Author(s):  
Zuzana Fremuntova ◽  
Tibor Mosko ◽  
Jakub Soukup ◽  
Johanka Kucerova ◽  
Marie Kostelanska ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Cellular Prion Protein ◽  
Neuronal Cell Line ◽  
Expression Processing

scholarly journals Cellular Prion Protein and Caveolin-1 Interaction in a Neuronal Cell Line Precedes Fyn/Erk 1/2 Signal Transduction

2006 ◽  
Vol 2006 ◽  
pp. 1-13 ◽  
Author(s):  
Mattia Toni ◽  
Enzo Spisni ◽  
Cristiana Griffoni ◽  
Spartaco Santi ◽  
Massimo Riccio ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Cellular Prion Protein ◽  
Glutathione S Transferase ◽  
Neuronal Cell Line ◽  
Caveolin 1 ◽  
Fyn Kinase

It has been reported that cellular prion protein (PrPc) is enriched in caveolae or caveolae-like domains with caveolin-1 (Cav-1) participating to signal transduction events by Fyn kinase recruitment. By using the Glutathione-S-transferase (GST)-fusion proteins assay, we observed that PrPc strongly interacts in vitro with Cav-1. Thus, we ascertained the PrPc caveolar localization in a hypothalamic neuronal cell line (GN11), by confocal microscopy analysis, flotation on density gradient, and coimmunoprecipitation experiments. Following the anti-PrPc antibody-mediated stimulation of live GN11 cells, we observed that PrPc clustered on plasma membrane domains rich in Cav-1 in which Fyn kinase converged to be activated. After these events, a signaling cascade through p42/44 MAP kinase (Erk 1/2) was triggered, suggesting that following translocations from rafts to caveolae or caveolae-like domains PrPc could interact with Cav-1 and induce signal transduction events.

Cellular prion protein suppresses the apoptosis in a neuronal cell line established from type-1 prion protein gene-deficient mice

Prions ◽  
2006 ◽  
pp. 203-203
Author(s):  
Keiichi Saeki ◽  
Takuya Nishimura ◽  
Akikazu Sakudo ◽  
Yoshitsugu Matsumoto ◽  
Takashi Onodera
Keyword(s):  
Cell Line ◽  
Prion Protein ◽  
Protein Gene ◽  
Neuronal Cell ◽  
Cellular Prion Protein ◽  
Prion Protein Gene ◽  
Neuronal Cell Line ◽  
Deficient Mice

Glycosylation-related genes are variably expressed depending on the differentiation state of a bioaminergic neuronal cell line: implication for the cellular prion protein

2008 ◽  
Vol 26 (4) ◽  
pp. 477-493 ◽  
Author(s):  
Myriam Ermonval ◽  
Daniel Petit ◽  
Aurélien Le Duc ◽  
Odile Kellermann ◽  
Paul-François Gallet
Keyword(s):  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Cellular Prion Protein ◽  
Neuronal Cell Line

scholarly journals Serum Withdrawal-Induced Apoptosis in ZrchI Prion Protein (PrP) Gene-Deficient Neuronal Cell Line Is Suppressed by PrP, Independent of Doppel

2007 ◽  
Vol 51 (4) ◽  
pp. 457-466 ◽  
Author(s):  
Takuya Nishimura ◽  
Akikazu Sakudo ◽  
Yoriko Hashiyama ◽  
Akiko Yachi ◽  
Keiichi Saeki ◽  
...  
Keyword(s):  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Neuronal Cell Line ◽  
Serum Withdrawal ◽  
Induced Apoptosis ◽  
Prp Gene

Neurotoxic Prion Protein (PrP) Fragment 106-126 Requires the N-terminal Half of the Hydrophobic Region of PrP in the PrP-Deficient Neuronal Cell Line

2007 ◽  
Vol 14 (1) ◽  
pp. 1-6
Author(s):  
Akikazu Sakudo ◽  
Izuru Nakamura ◽  
Deug-Chan Lee ◽  
Keiichi Saeki ◽  
Kazuyoshi Ikuta ◽  
...  
Keyword(s):  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Hydrophobic Region ◽  
Neuronal Cell Line

Impairment of superoxide dismutase activation by N-terminally truncated prion protein (PrP) in PrP-deficient neuronal cell line

2003 ◽  
Vol 308 (3) ◽  
pp. 660-667 ◽  
Author(s):  
Akikazu Sakudo ◽  
Deug-chan Lee ◽  
Keiichi Saeki ◽  
Yuko Nakamura ◽  
Keiichi Inoue ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Cell Line ◽  
Prion Protein ◽  
Neuronal Cell ◽  
Neuronal Cell Line
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