1α,25‐Dihydroxyvitamin D3Regulates the Expression of Membrane‐Type Matrix Metalloproteinase‐1 in Normal Human Osteoblast‐like Cells

2003 ◽  
Vol 29 (3) ◽  
pp. 353-362 ◽  
Author(s):  
Xiang‐hang Luo ◽  
Er‐yuan Liao
Keyword(s):  
Matrix Metalloproteinase ◽  
Human Osteoblast ◽  
Matrix Metalloproteinase 1 ◽  
Normal Human ◽  
Membrane Type ◽  
Normal Human Osteoblast

Abstract 172: Lumican-null Mice Are Susceptible to Aging and Isoproterenol-induced Myocardial Fibrosis

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Pao-Hsien Chu
Keyword(s):  
Matrix Metalloproteinase ◽  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Myocardial Fibrosis ◽  
Cardiac Remodeling ◽  
Cardiac Fibrosis ◽  
Systolic Function ◽  
Wild Type ◽  
Matrix Metalloproteinase 1 ◽  
Membrane Type

Aims: With aging and stresses, the myocardium undergoes structural remodeling and often leading to fibrosis. Main Methods: To examine whether lumican, one of the class II small leucine-rich proteoglycans, has a role in cardiac remodeling and fibrosis, we analyzed the basic cardiac phenotypes of lumican-null (Lum-/-) mice in both youth and elder, and then used the isoproterenol-induced cardiac fibrosis model to study the roles of extra-cellular matrix and apoptosis in cardiac remodeling. Key Findings: Higher mortality resulted from significantly impaired systolic function, and an increase of atrial natriuretic peptide secreted by the ventricles in response to excessive stretching of myocytes of Lum-/- mice in comparison to wild type littermates. In addition, Lum-/- mice exhibited higher level of TGF-β, collagen I/III, and membrane-type matrix metalloproteinase-1 (MT1-MMP, or MMP-14) during cardiac remodeling. Significance: Our data implicates that the lumican protein plays an important role in the pathogenesis of cardiac fibrosis.

scholarly journals Membrane-type matrix metalloproteinase-1(MT1-MMP) gene is overexpressed in highly invasive hepatocellular carcinomas

1998 ◽  
Vol 28 (2) ◽  
pp. 231-239 ◽  
Author(s):  
Tomika Harada ◽  
Shigeki Arii ◽  
Masahiro Mise ◽  
Takashi Imamura ◽  
Hiroaki Higashitsuji ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Hepatocellular Carcinomas ◽  
Matrix Metalloproteinase 1 ◽  
Membrane Type

Equine laminitis: Membrane type matrix metalloproteinase-1 (MMP-14) is involved in acute phase onset

2008 ◽  
Vol 40 (5) ◽  
pp. 482-487 ◽  
Author(s):  
M. T. Kyaw-Tanner ◽  
O. Wattle ◽  
A. W. Eps ◽  
C. C. Pollitt
Keyword(s):  
Matrix Metalloproteinase ◽  
Acute Phase ◽  
Matrix Metalloproteinase 1 ◽  
Membrane Type

Adhesion and Cell Viability of Normal Human Osteoblasts (NHOst) on Scaffolds of Poly (3-hydroxybutyrate)

2015 ◽  
Vol 1721 ◽  
Author(s):  
Maraolina Domínguez-Díaz ◽  
Angelica Meneses-Acosta ◽  
Angel Romo-Uribe
Keyword(s):  
Contact Angle ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Human Osteoblast ◽  
Cell Nuclei ◽  
Electrospun Membrane ◽  
Osteoblast Cells ◽  
Cast Film ◽  
Normal Human ◽  
Normal Human Osteoblast

ABSTRACTBiodegradable Normal Human Osteoblast (NHOst) cells were inoculated into the polymer scaffolds of poly(β-hydroxybutyrate) (PHB) obtained from a specially developed strain of Azotobacter vinelandii. Cell adhesion is essential to promote growth on scaffolds for tissue engineering. Thus, in this research we focused on the adhesion of osteoblast cells to PHB scaffolds produced by solution casting and electrospinning. Cell viability was also investigated up to 168 hrs. Water contact angle on the PHB scaffolds was determined prior to the cells inoculation. The contact angle is usually related to the ability of different cell strains to adhere to a given material. The as cast film exhibited a contact angle α=72° whereas for the electrospun membrane α=102°, thus in theory cell adhesion would be greater for the cast film. Biological testing was carried out on plates of 24 wells; cell viability was determined by Trypan Blue, cell morphology by optical microscopy, and cell nuclei integrity by staining with Acridine orange. Parallel studies were carried out on control (empty) wells. Microscopy observations 168 hrs after cell inoculation showed larger quantities of osteoblast cells in the wells containing PHB scaffolds and the cell nuclei were still active. Moreover, it was found that the cells grew inside the PHB scaffolds and the cell viability was slightly greater for the electrospun scaffold. Interestingly, the time to remove the cells from the scaffolds (film and membranes) was increasing function of the cell culture time, therefore suggesting that PHB promotes adhesion of Normal Human Osteoblast cells to its surface.

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