Determination of endometrial prolactin in vivo as a marker for endometrial development in spontaneous ovulatory cycles and in vitro fertilization cycles

2001 ◽  
Vol 15 (3) ◽  
pp. 210-218
Author(s):  
J. J. M. L. Dekker ◽  
F. Martens ◽  
J. Schoemaker
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization

scholarly journals In Vitro Fertilization of In Vitro and In Vivo Matured Oocytes from Prepubertal Meishan Gilts

1990 ◽  
Vol 61 (11) ◽  
pp. 1011-1016
Author(s):  
Takashi MIYANO ◽  
Kiyoshi YOSHIKAWA ◽  
Seishiro KATO ◽  
Hiroshi HARAYAMA ◽  
Iwao NANJO ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization

scholarly journals Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report

2020 ◽  
Vol 76 (03) ◽  
pp. 6356-2020 ◽  
Author(s):  
KATARZYNA PONIEDZIAŁEK-KEMPNY ◽  
BARBARA GAJDA ◽  
IWONA RAJSKA ◽  
LECHOSŁAW GAJDA ◽  
ZDZISŁAW SMORĄG
Keyword(s):  
Case Report ◽  
In Vitro Fertilization ◽  
Control Group ◽  
First Birth ◽  
Vitro Fertilization ◽  
Research Material ◽  
Preliminary Study ◽  
Experimental Group

The aim of the study was to examine the in vivo viability of in vitro-produced (IVP) porcine embryos obtained from oocytes matured with thymosin. The research material for this study consisted of immature pig oocytes obtained from ovaries after slaughter and ejaculated semen obtained from one boar. The immature oocytes were cultured in vitro until the metaphase II stage in a medium supplemented with thymosin (TMS). The presumptive zygotes obtained were cultured in vitro for 4-40 hours. The presumptive zygotes and 2-4-cell embryos were evaluated in vivo after transferring them to synchronized recipients. After the transfer of embryos from the experimental group into 2 recipients (50 embryos into each gilt) and the transfer of 50 embryos from the control group into 1 recipient, both gilts that had received embryos obtained by in vitro fertilization of oocytes matured with TMS became pregnant and delivered a total of 16 live piglets. After the transfer of embryos from the control group, no pregnancy was achieved. In conclusion, the results of our preliminary study suggest that the maturation of pig oocytes with thymosin supports the in vivo survival of in vitro produced embryos. It is important to note, that this was the first birth of piglets obtained after transfer of IVP embryos in Poland.

Kinetic immunonephelometric determination of protein concentrations in follicular fluid.

1987 ◽  
Vol 33 (7) ◽  
pp. 1185-1189 ◽  
Author(s):  
F Gulamali-Majid ◽  
S Ackerman ◽  
L Veeck ◽  
A Acosta ◽  
P Pleban
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Antithrombin Iii ◽  
Oocyte Maturity ◽  
Vitro Fertilization ◽  
Analytical Recovery ◽  
Alpha 1 Antitrypsin ◽  
Respective Protein

Abstract Nonsteroidal biochemical markers of oocyte maturity could presumably aid in oocyte selection and in the timing of insemination for in vitro fertilization. We assessed the usefulness of six potential markers found in follicular fluid. We used kinetic immunonephelometry to measure concentrations of alpha 1-antitrypsin, alpha 2-macroglobulin, antithrombin III, ceruloplasmin, fibrinogen, and plasminogen in 53 fluids and the corresponding plasma from 20 women undergoing in vitro fertilization. Specimens were obtained from both mature and immature follicles from each woman. The respective protein concentrations in follicular fluid could be assayed with a between-assay CV of 1.5% to 3.2%. Analytical-recovery studies indicated that only fibrinogen and antithrombin III concentrations were altered (by 120% and 75%, respectively) during aspiration of the follicle into saline. All protein concentrations were significantly increased in mature follicles, as were the follicular fluid:plasma concentration ratios for alpha 1-antitrypsin, antithrombin III, and ceruloplasmin.

In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro

1986 ◽  
Vol 25 (1) ◽  
pp. 146 ◽  
Author(s):  
W.T.K. Cheng ◽  
R.M. Moor ◽  
C. Polge
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization
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